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http://dx.doi.org/10.6116/kjh.2013.28.1.23.

Anti-obesity effects of herbal extract YY312 in C57BL/6 mice fed a high-fat diet and 3T3-L1 cells  

Kang, Insug (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Hwang, Keun-Young (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Choi, A-Young (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Roh, Kughwan (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Choi, Ji Hyun (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
Sim, Yeomoon (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Park, Yoo Kyoung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Oh, Myung Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.28, no.1, 2013 , pp. 23-31 More about this Journal
Abstract
Objectives : The purpose of this study was to determine the anti-obesity effect and molecular mechanism of YY312, a herbal extract composed of Imperatae Rhizoma, Citri Unshius Pericarpium Immaturus, and Evodiae Fructus, on a high-fat diet-induced animal model and on 3T3-L1 cells. Methods : C57BL/6 mice were fed for 6 weeks with a normal diet or a high-fat diet (HFD). Then they orally administered daily with 300 mg/kg YY312 for next 10 weeks. Body weight and food consumption were recorded weekly and daily, respectively. Tissue weights, serum lipid, and glucose levels were analyzed at the end of the study. Additionally, the effects of YY312 on adipocyte differentiation in 3T3-L1 cells were examined. After differentiating 3T3-L1 cells were treated with YY312, Oil-red O staining, RT-PCR, and Western blotting were performed for lipid accumulation, mRNA expression of adipogenesis gene, and AMP-activated protein kinase (AMPK) phosphorylation, respectively. Results : YY312-administered mice showed a significant reduction of body weights and abdominal adipose tissue weights. YY312 also reduced the serum levels of triglycerides and total cholesterol, compared with the HFD group. Treatment with YY312 inhibited lipid accumulation and blocked expression of adipogenic transcription factors and lipogenesis genes, including peroxisome proliferator-activated receptor ${\gamma}$, CCAT/enhancer binding protein ${\alpha}$ and fatty acid synthase. YY312 increased AMPK phosphorylation in 3T3-L1 adipocytes. Conclusions : This study showed that herbal extract YY312 has an anti-obesity effect in vitro and in vivo. Thus, YY312 could be developed as a supplement for reduction of body weight gain induced by an HFD.
Keywords
YY312; high-fat diet; obesity; C57/BL6 mice; body weight; 3T3-L1 adipocyte differentiation;
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Times Cited By KSCI : 5  (Citation Analysis)
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