Browse > Article
http://dx.doi.org/10.6116/kjh.2012.27.4.45

Effect of WHW, a polyherbal medicine for the treatment of chronic renal failure on staurosporin-induced apotosis in MDCK cells  

Bae, Hyo-Sang (Department of Sassang Constitunal Medicine, Dongguk University Ilsan Oriental Hospital)
Yoon, Cheol-Ho (Department of Internal Medicine, Orient Medical College, Dongguk University Bundang Oriental Hospital)
Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
Publication Information
The Korea Journal of Herbology / v.27, no.4, 2012 , pp. 45-51 More about this Journal
Abstract
Objectives : WHW is a polyherbal medicine for the treatment of chronic renal failure (CRF). WHW previously reported various biological property such as anti-inflammation, anti-oxidation and anti-renal fibrosis in CRF. This study aimed to investigate the anti-apoptotic effect of WHW on staurosporin(SSP)-induced apoptosis in canine kidney epithelial cells (MDCK). Methods : MDCK cells were treated with different concentrations of WHW (0.1, 0.2, 0.5 and $1mg/m{\ell}$) for 1 h, and then induced apoptosis by treatment of SSP ($1{\mu}M$) for 24 h. Cell viability was measured by WST-1 assay. The expression of apoptotic proteins such as caspase-3, Bax and Bcl-2 was determined by Western blot. Caspase-3 activity and ROS levels were also measured by their commercial available assay kits. Cell apoptosis was observed by Hoechst and DNA fragmentation. Results : WHW significantly increased the cell viability on SSP-treated MDCK cells. WHW inhibited SSP-induced expression of apoptotic proteins such as caspase-3 and Bax, and significantly decreased caspase-3 activity in MDCK cells. WHW significantly decreased SSP-induced production of ROS, and suppressed SSP-induced chromatin condensation and DNA fragmentation in MDCK cells. Conclusions : These results suggest that WHW has an anti-apoptotic effect in renal cells through suppressing the expression of apoptotic proteins, ROS production and DNA damages.
Keywords
Anti-apoptosis; chronic renal failure; MDCK; polyherbal medicine; staurosporin; WHW;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Han YK, Park YK. Effect of Atractylodis Rhizoma Alba water extract on streptozotocin-induced diabetes in rats. Kor J Herbology. 2011 ; 26(4) : 23-30.
2 Khan SM, Dauffenbach LM, Yeh J. Mitochondria and caspases in induced apoptosis in human luteinized granulosa cells. BBRC. 2000 ; 269 : 542-5.
3 Reed JC. Proapoptotic multidomain Bcl-2/Bax-family proteins : mechanisms, physiological roles, and therapeutic opportunities. Cell Death Differ. 2006 ; 13 : 1378-86.   DOI   ScienceOn
4 Halliwell B, Gutteridge JMC. Protection aganist oxidants in biological systems : the superoxide theory of oxygen toxicity. Free Radicals in Biology and Medicine. 2nd ed. Oxford: Clarendon Press. 1989 ; 86-9.
5 Jung HW, Jung JK, Ramalingam M, Yoon CH, Bae HS, Park YK. Anti-diabetic effect of Wen-pi-tang-Hab-Wu-ling-san extract in streptozotocin-induced diabetic rats. Indian J Pharmacol. 2012 ; 44 : 97-102.   DOI   ScienceOn
6 Park YK, Oh TW, Bae HS, Yoon CH. Thirteen-week repeated-dose oral toxicity study of the Modified Wenpitang-Hab-Wulingsan(WHW(R)) in Sprague-Dawley rats. Kor J Herbology. 2010 ; 25(3) : 43-51.   과학기술학회마을
7 Gobe G, Willgoss D, Hogg N, Schoch E, Endre Z. Cell survival or death in renal tubular epithelium after ischemia-reperfusion injury. Kidney Int. 1999 ; 56 : 1299-304.   DOI   ScienceOn
8 Sen K, Lindenmeyer MT, Gaspert A, Eichinger F, Neusser MA, Kretzler M, Segerer S, Cohen CD. Periostin is induced in glomerular injury and expressed de novo in interstitial renal fibrosis. Am J Pathol. 2011 ; 179 : 1756-67.   DOI   ScienceOn
9 Couser WG, Nangaku M. Cellular and molecular biology of membranous nephropathy. J Nephrol. 2006 ; 19 : 699-705.
10 Busuioc M, Gusbeth-Tatomir P, Covic A. The management in chronic kidney disease (CKD). Rev Med Chir Soc Med Nat Iasi. 2008 ; 112 : 896-901.
11 Feng JX, Li HY, Liu ZQ, Zhou ZM, Tian JW, Liang M. Effect of the ethanol extract of Picrorhiza scrophulariiflora on the progression of chronic kidney disease in a rat remnant kidney model. Nan Fang Yi Ke Da Xue Xue Bao. 2010 ; 30 : 1505-8.
12 Zhu YF, Chen YP, Rui HL, Dong HR, Hu Z. Protective effects of Hirsutella sinensis on renal interstitial fibrosis : experiment with rat model of chronic aristolochic acid nephropathy. Zhonghua Yi Xue Za Zhi. 2007 ; 87 : 2667-71.
13 Kowaltowski AJ, Vercesi AE. Mitochondrial damage induced by conditions of oxidative stress. Free Radic Biol Med. 1999 ; 26 : 463-71.   DOI   ScienceOn
14 Kang YH, Kwon OS, Choi HS, Lee JH. Effect of the Oryungsan on the injured liver in CCL4-treated rats. The Journal of Dong Guk Oriental Medicine. 1996 ; 5 : 131-47.
15 Lee JJ, Lee TW, Chang HH, Kim HJ, Ihm CG, Kim MJ. Inhibitory effects of Siryungtang and Onbitang on oxidation of low density lipoprotein. The Kor J Nephrology. 2001 ; 20 : 478-85.
16 Lee YK, Kang SB. The research study on the symptoms of Wuling-san compared with other 5 herbal medicines-based on Kangpind-Shanghanlun. The Journal of East-West Medicine. 2011 ; 36 : 1-9.
17 Jung KJ, Kim J, Park YK, Yoon YR, Park KM. Wen-pi-tang-Hab-Wu-ling-san reduces ureteral obstructive renal fibrosis by the reduction of oxidative stress, inflammation, and TGF-beta/Smad2/3 signaling. Food Chem Toxicol. 2010 ; 48 : 522-9.   DOI   ScienceOn
18 Lee SI, Kim HJ, Baek MC, Park KM, Park Y, Yoon CH, Boo YC. Wen-pi-tang-Hab-Wu-ling-san, an oriental herbal prescription, attenuates epithelial-mesenchymal transdifferentiation stimulated by TGF-beta1 in kidney cells. Phytother Res. 2007 ; 21 : 548-53.   DOI   ScienceOn
19 Seok YM, Kim J, Choi KC, Yoon CH, Boo YC, Park Y, Park KM. Wen-pi-tang-Hab-Wu-ling-san attenuates kidney ischemia/reperfusion injury in mice. A role for antioxidant enzymes and heat-shock proteins. J Ethnopharmacol. 2007 ; 112 : 333-40.   DOI   ScienceOn
20 Seok YM, Kim J, Park MJ, Boo YC, Park YK, Park KM. Wen-pi-tang-Hab-Wu-ling-san attenuates kidney fibrosis induced by ischemia/reperfusion in mice. Phytother Res. 2008 ; 22 : 1057-63.   DOI   ScienceOn
21 Jung HW, Yoon CH, Kim YH, Boo YC, Park KM, Park YK. Wen-Pi-Tang-Hab-Wu- Ling-San extract inhibits the release of inflammatory mediators from LPS-stimulated mouse macrophages. J Ethnopharmacol. 2007 ; 114 : 439-45.   DOI   ScienceOn
22 Bae HS, Nam JS, Jung JK, Oh SY, Park YK. Anti-diabetic effect of Wen-pi-tang-Hab-Wu-ling-san extract in streptozotocin-induced diabetic rats. Kor J Herbology. 2008 ; 23(3) : 85-91.   과학기술학회마을
23 Blagg CR, Scribner BH. Diet, drugs and dialysis in the management of chronic renal failure. Prog Biochem Pharmacol. 1972 ; 7 : 452-97.
24 Paweletz N. Walther Flemming: pioneer of mitosis research. Nat Rev Mol Cell Biol. 2001 ; 2 : 72-5.   DOI   ScienceOn
25 Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007 ; 35 : 495-516.   DOI   ScienceOn
26 Kim SK. Chronic renal failure. The Korea National Institute of Health, Korea centers for disease control & prevention. 2007 ; 21 : 1-15.
27 Nam HS, Cho CS, Kim CJ. A study on the healing mechanism of herbal medicine, Oryoungsan. Daejeon university Institute of Oriental Medicine. 2001 ; 10 : 157-66.