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http://dx.doi.org/10.6116/kjh.2012.27.3.89

Effects of Salvia Miltriorrhiza Radix on Neuronal Apoptosis following Intracerebral Hemorrhage of Rats  

Lee, Hung-Gi (Department of Anatomy-Pointology, College of Oriental Medicine, Gachon University)
Kim, Youn-Sub (Department of Anatomy-Pointology, College of Oriental Medicine, Gachon University)
Publication Information
The Korea Journal of Herbology / v.27, no.3, 2012 , pp. 89-94 More about this Journal
Abstract
Objects : The purpose of this study was to observe the effect of Salviae Miltriorrhiza Radix(SMR) water-extract on intracerebral hemorrhage(ICH) and neuronal apoptosis in the injured areas. Method : ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in Sprague-Dawley rats. The rats were givened oral SMR treatment once a day for three days after the ICH treatment. TUNEL positive cells in the affected regions were performed by TUNEL assay, Bax and Bcl-2 positive cells by immunohistochemistry and the Bax expression by western blotting method. Results : The results are as follow; 1. SMR significantly reduced the number of TUNEL positive cells in the peri-hematoma reigions of ICH-induced rats. 2. SMR significantly reduced the number of Bax positive cells in the peri-hematoma regions of ICH-induced rats. 3. SMR did not affect the number of Bcl-2 positive cells in the peri-hematoma regions of ICH-induced rats. 4. SMR significantly reduced the Bax expressions compared with ICH group in hemorrhagic hemisphere of ICH-induced rats. Conclusion : These results suggest that SMR is effective in reducing neuronal apoptosis.
Keywords
Salvia Miltriorrhiza Radix(SMR); Collagenase; TUNEL; Bax; Bcl-2;
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