Browse > Article
http://dx.doi.org/10.6116/kjh.2012.27.2.61

Neuroprotective effect of the water extract of Angelicae Gigantis Radix Palva in ischemic stroke rats  

Park, Ki-Ho (Department of Herbology, College of Oriental Medicine, Dongguk University)
Oh, Tae-Woo (Department of Herbology, College of Oriental Medicine, Dongguk University)
Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
Publication Information
The Korea Journal of Herbology / v.27, no.2, 2012 , pp. 61-67 More about this Journal
Abstract
Objective : Angelica Gigas Nakai is a popular oriental medicine used for the treatment of vascular diseases. The aim of this study is to investigate neuroprotective effect of the water extract of Anelicae Gigantis Radix Palva (AG) in transient middle cerebral artery occlusion (tMCAO)-induced ischemic rats via the regulation of angiogenesis-related molecules. Methods : Sprague-Dawley rats were intraperitoneally administrated with AG water extract at doses of 10, 25, 50 mg/kg body weight after tMCAO (90 min occlusion). reperfusion for 24 hr infarction volumes were measured by 2,3,5-tetrazolium chloride (TTC) staining. Brain tissues were observed neuronal cell injuries by nissl staining, and also brain-blood barrier (BBB) permeability change by evans blue. Expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and Tie-2 receptor protein in brain tissues was determined by western blot. Results : AG water extract significantly reduced infarction volume in ischemic brains of rats, degradation of neuronal cell, BBB permeability and expression of VEGF protein dose-dependently. Ang-1 protein was increased dose-dependantly, not significantly. Conclusion : This study suggests that AG water extract shows neuroprotective effect by preventing BBB breakdown, with regulating angiogenesis factor VEGF and Ang-1.
Keywords
Angelica gigas; middle cerebral artery occlusion; brain-blood barrier; vascular endothelial growth factor; angiopoietin-1;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Valable S, Montaner J, Bellail A, Berezowski V, Brillault J, Cecchelli R, Divoux D, MacKenzie ET, Bernaudin M, Roussel S, Petit E. VEGF-induced BBB permeability is associated with an MMP-9 activity increase in cerebral ischemia: both effects decreased by Ang-1. J Cereb Blood Flow Metab, 2005;25:1491-504.   DOI   ScienceOn
2 Yancopoulos GD, Davis S, Gale NW, Rudge JS, Weigand SJ, Holash J. Vascular-specific growth factors and blood vessel formation. Nature 2000; 407: 242-8.   DOI   ScienceOn
3 Zhang Z, Chopp M. Vascular endothelial growth factor and angiopoietins in forcal cerebral ischemia, Trands Cardiovasc Med. 2002;12(2): 62-66.   DOI   ScienceOn
4 Oh HS, Kim HH, Ahn DK, Choi HY. Comparative studies on the angiogenic activity of water extract of Angelica Gigantis Radix, Angelica Sinensis Radix and Angelica Radix. Kor. J. Herbology 2001;16(2):19-27.
5 Song BK, Jeon YC, Kim SA, Shim AN, Seong KM, Lee EJ. The Effect of Intravenous Injection of the Water Extract of Angelica gigas Nakai on Gliosis in the Middle Cerebral Artery Occlusion Rats. J pharmacopuncture, 2011;14(3):5-17.
6 Ahn SD, Shin KH. Study on the Constituents of Angelica gigas root cultivated by short-time growing seeding. Korean J Plant Res 1996;9(2): 197-201.
7 Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible Middle cerebral artery occlusion without craniectomy in rats. Stroke 1989;20:84-91.   DOI   ScienceOn
8 The 9th Korean Pharmacopeia. Korea Food & Drug Administration. 2011:916-7.
9 Ahn MJ, Lee MK, Kim YC, Sung SH. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J. Pharm. Biomed. Anal. 2008;46:258-66.   DOI   ScienceOn
10 Jung MH, Lee SH, Ahn EM, Lee YM. Decursin and decursinol angelate inhibit VEGF-induced angiogenesis via suppression of the VEGFR-2-signaling pathway. Carcinogenesis 2009;30(4):655-61.   DOI   ScienceOn
11 Kim JH, Kim JH, Lee YM, Ahn EM, Kim KY, Yu YS. Decursin inhibits VEGF-mediated inner blood- retinal barrier breakdown by suppression of VEGFR-2 activation. J Cereb Blood Flow Metab 2009;29:1559-567.   DOI   ScienceOn
12 Kim SA, Oh HK, Kim JY, Hong JW, Cho SI. A Review of Pharmacological Effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their Bioactive Compounds. J Korean Oriental Med 2011;32(4):1-24.
13 Cechetto DF, Experimental cerebral ischemic lesions and autonomic and cardiac effects in cats and rats. Stroke 1993;24:16-9
14 Sun FY, Gu X. Molecular and Cellular Mechanisms of Neuroprotection by Vascular Endothelial Growth Factor. J Neurosci Res 2005;79:180-4.   DOI   ScienceOn
15 Hansen TM, Moss AJ, Brindle NPJ. Vascular Endothelial Growth Factor and Angiopoietins in Neurovascular Regeneration and Protection Following Stroke. Curr Neurovasc Res. 2008;5: 236-45.   DOI   ScienceOn
16 Kim HH, Lee JH, Lee JH, Ann DK, Park SK. Vasodilation effect of the water extract of three angelica spicies and four-substance decoctions in rat thoracic aorta. Kor. J. Herbology. 2011;16( 2):29-34.
17 Lee YG, Kim JK. The study of the oriental medicine extract on the hair growth effect : I. The effect of the mixture extract of Polygoni multiflori Radix, Angelicae gigantis Radix and Lycii Fructus on the hair growth. Kor. J. Herbology 2004;19(2):83-90.
18 Jeon YI, Park CS, Park CG. An Experimental Study of Effect on Brain Damage and Neuroprotective Effect of Angelicae Gigantis Radix extract against Cerebral Ischemia in Rats. Kor. J. Herbology 2003;18(4):25-35.
19 Kang SY, Kim YC. Decursinol and decursin protect primary cultured rat cortical cells from glutamate-induced neurotoxicity. J Pharm Pharmacol. 2007;59(6):863-70.   DOI   ScienceOn
20 Kwon JM, Moon YG, Kim YS, Jung JY, Ha YL, Yang JK. Effects of Extraction Methods of Medicinal Plants on Human Growth of Neuroblastoma SK-N-SH Cells. J Life Sci 2011; 21(8):1190-8.   DOI   ScienceOn
21 2010 Causes of Death Statistics. 2011. Statistics Korea.
22 Kang SY, Lee KY, Sung SH, Kim YC. Four new neuroprotective dihydropyranocoumarins from Angelica gigas. J Nat Prod. 2005;68(1):56-9.   DOI   ScienceOn
23 Kang SY, Kim YC. Neuroprotective Coumarins from the Root of Angelica gigas: Structure-Activity Relationships. Arch Pharm Res. 2007;30(11):1368-73.   DOI   ScienceOn
24 Kim YO, Ha NN, Boo YM, Park SY, Park JY, Yu YB, Shin JS, An DK, Kim H. Neuroprotective effect of Angelica gigas Extracts on the brain ischemia induced by four-vessel occlusion in rats. Kor. J. Herbology 2002;17(2);151-7.
25 2010 National Health Insurance Statistical Yearbook. 2011. National Health Insurance Corporation, Health Insurance Review & Assessment Service.
26 Son HY, Park YK. Neuroprotective effect of modified Boyanghwano-Tang and the major medicinal plants, Astragali Radix and Salviae Miltiorrhizae Radix on ischemic stroke in rats. Kor. J. Herbology 2010;25(2):71-9.
27 Kim YS, Park DW, Lee JY. Treatment of Brain Disease I, Analysis about technology development of Treatment and Business strategies, 2005 Issue Analysis for Commercialization of promicing items. Korea Institute of Science and Technology Information. 2005:46-7.
28 Professors of herbology in colleges of oriental medicine. Herbology. Yeonglimsa:Seoul. 2004: 632-4.
29 Lee KY, Kim EH, Kim GH, Heo JH, Lee BI, Sunwoo IN. Effect of Brain Hypothermia on Ischemic Injury and Matrix Metalloproteinase Activity in Focal Cerebral Ischemia. J Korean Neurol Assoc 2003;21(4):378-86.
30 Harhaj NS, Felinski EA, Wolpert EB, Sundstrom JM, Gardner TW, Antonetti DA. VEGF Activation of Protein Kinase C Stimulates Occludin Phosphorylation and contributes to Endothelial Permeability. Invest Ophthalmol Vis Sci 2006; 47(11):5106-15.   DOI   ScienceOn