Browse > Article
http://dx.doi.org/10.6116/kjh.2012.27.1.65

Nelumbinis Folium Methanol Extract Regulates Inducible Nitric Oxide Synthase Signaling Pathways in Mouse Peritoneal Macrophages  

Lee, Si-Eun (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Lee, Mi-Hwa (Department of Herbology, College of Oriental Medicine, KyungHee University)
Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, KyungHee University)
Sohn, Nak-Won (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Publication Information
The Korea Journal of Herbology / v.27, no.1, 2012 , pp. 65-71 More about this Journal
Abstract
Objective : Nelumbinis Folium (NF) is used for diarrhea, headache and dizziness in traditional medicine. In this paper, we examined the anti-inflammatory effects of the methanol extract of NF in mouse macrophages. Methods : Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with lipopolysaccharide(LPS) or LPS/interferon(IFN)-${\gamma}$ for viability assay, cytokine measurement and Western blotting. Results : NF methanol extract suppressed the levels of nitric oxide (NO) through reduction of inducible NO synthase in a concentration-dependent manner. The extract reduced LPS/IFN-${\gamma}$-stimulated STAT1 phosphorylation and LPS-induced $I{\kappa}B{\alpha}$ degradation through inhibition of $I{\kappa}B{\alpha}$ kinase activation. The extract also inhibited p38, JNK/SAPK and ERK1/2 activation. Conclusions : Our findings suggested that NF has anti-inflammatory activity, and have a potential for therapeutic application. Further research is required to investigate its anti-inflammatory active compounds.
Keywords
Nelumbinis Folium; inflammation; macrophages; nitric oxide synthase; signaling;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Huang CF, Chen YW, Yang CY, Lin HY, Way TD, Chiang W, Liu SH. Extract of lotus leaf(Nelumbo nucifera) and its active constituent catechin with insulin secretagogue activity. J Agric food chem. 2011;59(4):1087-94.   DOI   ScienceOn
2 Kim SM, Yun HJ, Yi HS, Won CW, Kim JE, Park SD. Nelumbo nucifera leaves inhibit HASMC proliferation and migration activated by TNF-$\alpha$. Kor J Herbology. 2009;24(4):77-86.
3 Yoshida H, Kisugi R. Mechanisms of LDL oxidation. Clinica Chimica Acta 2010;411:1875-1862.   DOI   ScienceOn
4 Jiangsu New Medical College, Chinese Materia Medica. 1th Jungdam. 1998;5957.
5 Zhang X, Mosser DM. Macrophage activation by endogenous danger signals. J of Pathology. 2008;214:161-78.   DOI   ScienceOn
6 Nathan C. Nitric oxide as a secretory product of mammalian cells. J. FASEB 1992;6:3051-64.   DOI
7 Abramson S, Amin A, Clancy R, Attur M. The role of nitric oxide in tissue destruction. Best Practice & Reasearch clinical rheumatology. 2001;15(5):831-45.   DOI   ScienceOn
8 Kleinert H, Pautz A, Linker K, Schwarz P. Regulation of the expression of inducible nitric oxide synthase. Eur J Pharmacol. 2004;500:255-66.   DOI   ScienceOn
9 Ihle J. STATs:signal transducers and activators of transcription. Cell. 1996;84:331-4.   DOI   ScienceOn
10 Simon A, Rai U, Fanburg B, Cochran B. Activation of the JAK-STAT pathway by reactive oxygen species. AJP Cell Physiology. 1998;275:1640-52.   DOI
11 Adcock I, Chung KF, Caramori G, Ito K. Kinase inhibitors and airway inflammation. Eur J Phamacol. 2006;533:118-32.   DOI   ScienceOn
12 Johnson G, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002;298:1911-2.   DOI   ScienceOn
13 Rawlings J, Rosler K, Harrison D. The JAK/STAT signaling pathway. J Cell Sci. 2004;117:1281-3.   DOI   ScienceOn
14 Ahn DG. Illustrated Book of Korean Medical Herbs. 7th ed. Kyohaksa. 1998:684.
15 Shin MG. Clinical Traditional Herbology. 4th ed. Younglimsa. 1994:358.
16 Kashiwada Y, Aoshima A, Ikeshiro Y, Hen YP, Furukawa H, Itoigawa M, Fujioka T, Mihashi K, Cosentino M, Natschke SM, Lee KS. Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids. Bioorg Med Chem. 2005;13(2):443-8.   DOI   ScienceOn
17 Agnihotri V, Elsohly H, Khan S, Jacob M, Joshi V, Smillie T, Khan I, Walker L. Constituents of Nelumbo nucifera leaves and their antimalarial and antifungal activity. Phytochemistry Lett. 2008;1(2):89-93.   DOI   ScienceOn
18 Kim KH, Chang SW, Ryu SY, Choi Su, Lee KR. Phytochemical constituents of Nelumbo nucifera. Natural Product Sciences. 2009;15(2):90-5.
19 Ohkoshi E, Miyazaki H, Shindo K, Watanabe H, Yoshida A, Yajima H. Constituents from the leaves of Nelumbo nucifera stimulate lipolysis in the white adipose tissue of mice. Planta Med. 2007;73(12):1255-9.   DOI   ScienceOn
20 Lin MC, Kao SH, Chung PJ, Chan KC, Yang MY, Wang CJ. Improvement for high fat diet-induced hepatic injuries and oxidative stress by flavonoid-enriched extract from Nelumbo nucifera leaf. J Agric Food Chem. 2009;57(13):5925-32.   DOI   ScienceOn
21 Ono Y, H Eri, Fukaya Y, Imai S, Ohizumi Y. Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J Ethnopharmacol. 2006;106(2):238-44.   DOI   ScienceOn
22 Khatami M. Inflammation, aging, and cancer: Tumoricidal versus tumorigenesis of immunity. Cell Biochem Biophysis. 2009;55:55-79.   DOI   ScienceOn
23 Du H, You JS, Zhao X, Park JY, Kim SH, Chang KJ. Antiobesity and hypolipidemic effects of lotus leaf hot water extract with taurine supplementation in rats fed a high fat diet. J Biomed Sci. 2010;17(1):S42.   DOI   ScienceOn
24 Jung HA, Jung YJ, Yoon NY, Jeong DM, Bae HJ, Kim DW, Na DH, Choi JS. Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation endproducts formation, and oxidative stress. Food Chem Toxicol. 2008;46(12):3818-26.   DOI   ScienceOn
25 Lawrence T, Willoughby D, Gilroy D. Anti-inflammatory lipid mediators and insights into the resolution of inflammation. Nat. Rev. Immunol. 2002;2:787-95.   DOI   ScienceOn
26 Forstermann U, Kleinert H. Nitric oxide synthase: expression and expressional control of the three isoforms. Naunyn-Schmiedeberg's Arch Pharmacol. 1995;352:351-64.
27 Julie B, Jaramillo M, Olivier M. Signalling events involved in interferon-$\gamma$-inducible macrophage nitric oxide generation. Immunology. 2003;108:513-22.   DOI   ScienceOn
28 Bosca L, Zeini M, Traves P, Hortelano S. Nitric oxide and cell viability in inflammatory cells:a role for NO in macrophage function and fate. Toxicology. 2005;208(2):249-58.   DOI   ScienceOn
29 Sankar G. Fulvio D, May M. Inhibition of Nuclear Factor Kappa B(NF-$\kappa$B):An emerging theme in anti-inflammatory therapies. Molecular interventions. 2002;2(1):22-35.   DOI   ScienceOn
30 Kaminska B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy-from molecular mechanisms to therapeutic benefits. Biochimica et Biophysica Acta. 2005;1754:253-62.   DOI   ScienceOn
31 Dirk B, Sorokin A, Dunn M. Multiple intracellular MAP kinase signaling cascades. Kidney International. 1996;49:1187-98.   DOI   ScienceOn