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http://dx.doi.org/10.6116/kjh.2012.27.1.65

Nelumbinis Folium Methanol Extract Regulates Inducible Nitric Oxide Synthase Signaling Pathways in Mouse Peritoneal Macrophages  

Lee, Si-Eun (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Lee, Mi-Hwa (Department of Herbology, College of Oriental Medicine, KyungHee University)
Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, KyungHee University)
Sohn, Nak-Won (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Publication Information
The Korea Journal of Herbology / v.27, no.1, 2012 , pp. 65-71 More about this Journal
Abstract
Objective : Nelumbinis Folium (NF) is used for diarrhea, headache and dizziness in traditional medicine. In this paper, we examined the anti-inflammatory effects of the methanol extract of NF in mouse macrophages. Methods : Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with lipopolysaccharide(LPS) or LPS/interferon(IFN)-${\gamma}$ for viability assay, cytokine measurement and Western blotting. Results : NF methanol extract suppressed the levels of nitric oxide (NO) through reduction of inducible NO synthase in a concentration-dependent manner. The extract reduced LPS/IFN-${\gamma}$-stimulated STAT1 phosphorylation and LPS-induced $I{\kappa}B{\alpha}$ degradation through inhibition of $I{\kappa}B{\alpha}$ kinase activation. The extract also inhibited p38, JNK/SAPK and ERK1/2 activation. Conclusions : Our findings suggested that NF has anti-inflammatory activity, and have a potential for therapeutic application. Further research is required to investigate its anti-inflammatory active compounds.
Keywords
Nelumbinis Folium; inflammation; macrophages; nitric oxide synthase; signaling;
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