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http://dx.doi.org/10.6116/kjh.2012.27.1.59

Effect of Forsythiae Fructus ethanol extract on inflammatory cytokine production and cellular signaling pathways in mouse macrophages  

Nam, Jung-Bum (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Lee, Mi-Hwa (Department of Herbology, College of Oriental Medicine, KyungHee University)
Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, KyungHee University)
Sohn, Nak-Won (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
Publication Information
The Korea Journal of Herbology / v.27, no.1, 2012 , pp. 59-64 More about this Journal
Abstract
Objective : This study was performed to evaluate the effect of Forsythiae Fructus (FF) ethanol extract on inflammatory cytokine production and its underlying mechanisms in mouse macrophages. Methods : Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with lipopolysaccharide(LPS) or LPS/interferon(IFN)-${\gamma}$ for cytokine measurement and cellular signaling molecule analysis. Results : FF ethanol extract decreased the levels of secreted tumor necrosis factor(TNF)-${\alpha}$ and interleukin(IL)-6 in IFN-${\gamma}$/LPS-stimulated cells in a concentration-dependent manner. FF extract reduced IFN-${\gamma}$/LPS-induced STAT1 phosphorylation and LPS-induced p38 and JNK activation, but not ERK1/2 activity. The extract also inhibited LPS-induced $I{\kappa}B{\alpha}$ degradation through suppression of $I{\kappa}B{\alpha}$ kinase. Conclusions : These results suggest that FF ethanol extract affects the production of TNF-${\alpha}$ and IL-6 through inhibition of activation of STAT-1, $I{\kappa}B{\alpha}$, p38, and JNK.
Keywords
Forsythiae Fructus; inflammation; macrophages; cytokine; signaling;
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1 Arjumand W, Seth A, Sultana S. Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NF$\kappa$B, TNF-$\alpha$ and caspase-3 expression in wistar rats. Food Chem Toxicol. 2011;49(9):2013-21.   DOI   ScienceOn
2 Hausmann M, Obermeier F, Paper DH, Balan K, Dunger N, Menzel K, Falk W, Schoelmerich J, Herfarth H, Rogler G. In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis. Clin Exp Immunol 2007;148(2):373-81.   DOI   ScienceOn
3 D'Acquisto F, May MJ, Ghosh S. Inhibition of Nuclear Factor Kappa B(NF-$\kappa$B):An Emerging Theme in Anti-Inflammatory Therapies. Mol Interv. 2002;2(1):22-35.   DOI   ScienceOn
4 Kono H, Rock KL. How dying cells alert the immune system to danger. Nature Rev. Immunol. 2008;8:279-289.   DOI   ScienceOn
5 hen CJ, Kono H, Goldenbock D, Reed G, Akira S, Rock KL. Identification of a key pathway required for the sterile inflammatory response triggerrered by dying cells. Nature Med 2007;13:851-856.   DOI   ScienceOn
6 Gordon S. The macrophage:past, present and future. Eur J Immunol 2007;37:S9-S17.   DOI   ScienceOn
7 Ramadori G, Damme JV, Rieder H, Buschenfelde KH. Interleukin 6, the third mediator of acute phase reaction, modulates hepatic protein synthesis in human and mouse. Comparison with interleukin $1\beta$ and tumor necrosis factor $-\alpha$. Eur J Immunol 1988;18:1259-1264.   DOI   ScienceOn
8 Aeberli D, Oertle S, Mauron H, Reichenbach S, Jordi B, Villiger PM. Inhibition of the TNF pathway:use of infliximab and etanercept as remission-inducing agents in cases of therapy=resistant chronic inflammatory disorders. Swiss Med Wkly 2002;132:414-422.
9 Kaminska B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy - from molecular mechanisms th therapeutic benefits. Biochimica et Biophysica Acta 2005;1754:253-262.   DOI   ScienceOn
10 Ershler WB, Keller ET. Age-associated increased interleukin-6 gene expression, late-life diseases, and frailty. Annu Rev Med. 200;51:245-270.   DOI   ScienceOn
11 Kou X, Qi S, Dai W, Luo D, Yin Z. Arctigenin inhibits lipopolysaccharide-induced iNOS expression in RAW264.7 cells through suppressing JAK-STAT signal pathway. Int Immunopharmacol. 2011;11(8):1095-1102.   DOI   ScienceOn
12 Jung HW, Mahesh R, Lee JG, Lee SH, Kim YS, Park YK. Pinoresinol from the fruits of Forsythia koreana inhibits inflammatory responses in LPS-activated microglia. Neurosci Lett. 2010;480(3):215-20.   DOI   ScienceOn
13 Herbology Association of Korea's Colleges of Oriental Medicine. Textbook of Herbology. Younglimsa. 1st ed. 1991;199.
14 Amber IJ, Hibbs JB Jr, Parker CJ, Johnson BB, Taintor RR, Vavrin Z. Activated macrophages conditioned medium:identification of the soluble factors inducing cytotoxicity and the L-arginine dependent effector mechanism J Leukoc Biol 1991;49:610-620.   DOI
15 Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation. Nat Rev Immunol 2008;8:958-969.   DOI   ScienceOn
16 Gasetel M, Kotlyarov A, Kracht M. Targeting innate immunity protein kinase signalling in inflammation. Nat Rev Drug Discuv 2009;8:480-499.   DOI   ScienceOn
17 Kim SY, Park YK. Ethanol extract of Forsythiae Fructus inhibits the production of inflammtory mediators in LPS-stimulated BV-2 microglia cells. Kor J Herbology 2008;23(3):93-102.
18 Rawlings JS, Rosler KM, Harrison DA. The JAK/STAT signaling pathway. J Cell Sci 2004;117:1281-1283.   DOI   ScienceOn