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http://dx.doi.org/10.6116/kjh.2011.26.4.009

The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice  

Do, Eun-Ju (Daegu Technopark Oriental Medicine Industry Support Center)
Hwang, Mee-Yul (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University)
Kim, Seung-Yeon (Daegu Technopark Oriental Medicine Industry Support Center)
Lee, Jin-Sang (Daegu Technopark Oriental Medicine Industry Support Center)
Yang, Dae-Seok (Yangnyeongsi People Co., Ltd.)
Yang, Chae-Ha (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University)
Kim, Mi-Ryeo (Daegu Technopark Oriental Medicine Industry Support Center)
Publication Information
The Korea Journal of Herbology / v.26, no.4, 2011 , pp. 9-14 More about this Journal
Abstract
Objective : Present study was carried out to investigate the effect of Gyungohkgo-gamibang extract on hair growth and protein expression in an alopecia model of C57BL/6 mice. Methods : Mice were divided into 3 experimental groups including normal (vehicle), Gyungohkgo-gamibang extract (YNS-10) and 5% minoxidil-treated group. The test materials were daily applied with 0.1 ml per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 5, 10, 15, 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by western blotting. Results : Application of minoxidil or YNS-10 stimulated the hair growth compared to vehicle treatment. Therefore hair density of minoxidil or YNS-10 application was increased about 200% and 210% more than in vehicle application on 14 day, respectively. And hair thickness of both minoxidil group and YNS-10 group was increased about 220% and 210 % more than in vehicle spreading on 14 day, respectively. Futhermore the protein expression of IGF-1 and VEGF were significantly up-regulated on 7 day in YNS-10 and minoxidil-spreaded group compared to vehicle-applied group. Conclusion : These data suggest that YNS-10 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hairrestore.
Keywords
hair growth; Gyungohkgo-gamibang; minoxidil; alopecia; IGF-1; VEGF;
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Times Cited By KSCI : 3  (Citation Analysis)
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