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http://dx.doi.org/10.6116/kjh.2011.26.2.045

The Effects of Nelumbo nucifera on Utraviolet-B Irradiated human Keratinocytes  

Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Ko, Eun-Bit (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Lee, Ho-Jin (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kim, Ju-Sung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kim, Jin-Soo (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Jee, Sung-Won (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kim, Hyu-Young (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Yeom, Myeong-Hoon (Skin Research Institute, R&D Center, AmorePacific Corporation)
Kim, Duck-Hee (Skin Research Institute, R&D Center, AmorePacific Corporation)
Kim, Han-Kon (Skin Research Institute, R&D Center, AmorePacific Corporation)
Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.26, no.2, 2011 , pp. 45-49 More about this Journal
Abstract
Objectives : The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts. Methods : Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes. Results : The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at $20mJ/cm^2$ UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen. Conclusions : These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.
Keywords
Ultraviolet-B (UVB); HaCaT keratinocyte; Nelumbo nucifera;
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Times Cited By KSCI : 3  (Citation Analysis)
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