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http://dx.doi.org/10.6116/kjh.2011.26.1.103

Standardization of Eleutherococcus species and HPLC Method Validation for Quantitative Analysis  

Song, Mi-Kyung (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University)
Kim, Mi-Yeon (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University)
Kim, Ho-Cheol (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.26, no.1, 2011 , pp. 103-110 More about this Journal
Abstract
Objective : For the standardization and quality control of eleutheroside E in Eleutherococcus species, HPLC analysis was performed and eleutherosdie E content was compared in 23 kinds of Eleutherococcus species collected from Korea and China. Methods : The content of eleutheroside E in stem bark of Eleutherococcus species collected from Korea and China were analyzed by HPLC. 0.5% phosphoric acid and acetonitrile was used as mobile solvent. Validation of HPLC analysis method was confirmed by analyzing specificity, linearity, precision and accuracy following ICH guideline. Results : Content of eleutheroside E was determined to be 1.0-1.6% and 0.5-0.8% in Korean and Chinese E. senticosus, respectively. Content of eleutheroside E in E. sessiliflorus was 0.7-1.1% and 0.2-0.4% respectively in Korean and Chinese origin. All calibration curves showed good linear regression. The method showed good precision and accuracy with intra-day and inter-day variations of 0.880-3.442% (RSD) and 0.606-3.328% (RSD), respectively, and average recovery was of 0.141-1.363% (RSD), for the eleutheroside E analyzed. Conclusion : These results might be used to establish a criterion of eleutheroside E in Eleutherococcus species.
Keywords
Eleutherococcus senticosus; Method validation; Standardization; Eleutheroside E; HPLC;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Ma XQ, Shi Q, Duan JA, Dong TX, Tsim KK. Chemical Analysis of Radix Astragali (Huangqi) in China : a comparison with its adulterants and seasonal variations. J. Agric. Food Chem, 2002 ; 50 : 4861-4866.   DOI   ScienceOn
2 Tohda Chihiro, Ichimura Mahoko, Bai Yanjing, Tanaka Ken, Shu Zhu, Katsuko Komatsu. Inhibitory effects of Eleutherococcus senticosus extracts on amyloid beta(25-35)-induced neuritic atrophy and synaptic loss. J. Pharmacol. Sci. 2008 ; 107 : 329-339.   DOI   ScienceOn
3 Fujikawa T, Yamaguchi A, Morita I, Takeda H, Nishibe S. Protective effects of Acanthopanax senticosus Harms from Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol Pharm Bull. 1996 ; 19 : 1227-1230.   DOI   ScienceOn
4 Bu YM, Rho SJ, Kim JM, Kim MY, Lee DH. Neuroprotective effect of tyrosol on transient focal cerebral ischemia in rats. Neurosci. Lett. 2007 ; 414 : 218-221.   DOI   ScienceOn
5 Takasugi N, Moriguchi T, Fuwa T, Sanada S, Ida Y, Shoji J, and Saito H. Effect of Eleutherococcus senticosus and its rectal temperature, body and grip tones, mortor coordination, and exploratory and spontaneous movements in acute stressed mice. Shoyakugaku Zasshi, 1985 ; 39 ; 232-237.
6 Hibasami H, Shohji T, Shibuya I, Higo K, Kanda T. Induction of apoptosis by three types of procyanidin isolated from apple (Rosaceae Malus pumila) in human stomach cancer KATO III cells. Int. J. Mol. Med. 2004 ; 13 : 795-799.
7 Kim HC, Textbook of herbal pharmacology, 1st rev ed. Seoul : Jipmoondang. 2001 : 204-205.
8 Gaffney BT, Hugell HM, Rich PA. Rich. The chromatographic co-elution of dihydrodehydrodiconiferyl alcohol monopyranose with eleutheroside E in Eleutherococcus senticosu s : implications for eleutheroside E assays. Phytochem Anal. 2004 ; 15 : 231-234.   DOI   ScienceOn
9 Huang LZ, Huang BK, Ye Q, Qin LP. Bioactivity-guided fractionation for anti-fatigue property of Acanthopanax senticosus. J. Ethnopharmacol. 2010 ; Article in press.
10 Park SH, Lee SG, Kang SK, Chung SH. Acanthopanax senticosus reverses fatty liver disease and hyperglycemia in ob/ob mice. Arch Pharm Res. 2006 ; 29 : 768-776.   DOI
11 Ahn JK, Lee WY, Oh SJ, Park YH, Hur SD, Choi MS. The contents of chlorogenic acid and Eleutheroside E in Eleutherococcus senticosus (Rupr. et Maxim) Harms. Jour. Korean For. Soc. 2000 ; 89 : 216-222.
12 Ester RC, Ballerinib G, Sequeiraa AF, Velascoa GA, Zalazara MF. Chemical composition of essential oil from Tagetes minuta. leaves and flowers. Journal of the Argentine Chemical Society. 2008 ; 96 : 80-86.
13 Jia, L. and Y. Zhao. Current evaluation of the millennium phytomedicine-ginseng (I) : etymology, pharmacognosy, phytochemistry, market and regulations. Curr. Med. Chem. 2009 ; 16 : 2475-2484.   DOI   ScienceOn
14 Tolonen A, Joutsamo T, Mattlla S, Kamarainen T, Jalonen J. Identification of isomeric dicaffeoylquinic acids from Eleutherococcus senticosus using HPLC-ESI/TOF/MS and 1H-NMR methods. Phytochem Anal. 2002 ; 13 : 316-328.   DOI   ScienceOn
15 Patrick N, Yat, John T. Arnason and Denins V C. Awang. An improved extraction procedure for the rapid, quantitative High-Performance Liquid Chromatographic estimation of the main eleutherosides (B and E) in Eleutherococcus senticosus (Eleuthero). Phytochemical Analysis. 1998 ; 9 : 291-295.   DOI   ScienceOn
16 Davydov M, Krikorian AD. Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as an adaptogen : a closer look. J Ethnopharmacol. 2000 ; 72 : 345-393.   DOI   ScienceOn
17 Lee EH, Cha BC. Quantitative Analysis of Acanthopanacis cortex and Achyranthis Radix for standardization of GCSB-5 preparation. Kor. J. Pharmacogn. 2008 ; 39 : 316-323.
18 Jeong HN, Lim SH, Kim HY, Kim KD, Park YH, Ham HJ, Lee KJ, Kim KH, Ahn YS. Quality changes in Eleutherococcus senticosus cortex processed by different pretreatment and drying method. Korean J. Medicinal Crop Sci. 2010 ; 18 : 98-104