Browse > Article
http://dx.doi.org/10.6116/kjh.2010.25.4.031

Neuroprotective Effects of Agrimoniae Herba against Intrastriatal Hemorrhage in Rats  

Choi, Young-Seuk (Department of Anatomy-Pointlogy College of Oriental Medicine, Kyungwon University)
Kim, Youn-Sub (Department of Anatomy-Pointlogy College of Oriental Medicine, Kyungwon University)
Publication Information
The Korea Journal of Herbology / v.25, no.4, 2010 , pp. 31-37 More about this Journal
Abstract
Objects : This study was performed in order to evaluate the effects of Agrimoniae herba (AH) ethanol extract on intrastriatal hemorrhage (ISH). Method : ISH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in Sprague-Dawley rats. AH was orally given once a day for 3 days after ISH. Hematoma volume and percentage edema were examined. Immunohistochemistry was processed for iNOS, c-Fos, MMP-9, and MMP-12 expressions in the brain sections and each immuno-labeling were calculated with image analysis. Results : results are as follows; 1. AH reduced the hematoma volume and percentage edema of the ISH-induced rat brain. 2. AH swollen apoptotic bodies and neurons in the peri-hematoma regions of the ISH-induced rat brain. 3. AH significantly reduced c-Fos, MMP-9 and MMP-12 positive cells in the peri-hematoma regions of the ISH-induced rat brain. 4. AH swollen iNOS expressions in the peri-hematoma regions of the ISH-induced rat brain. Conclusion : These results suggest that AH plays an anti-apoptotic neuroprotective effect through control of ISH, suppression of c-Fos, and down-regulation of MMP-9 and MMP-12 expressions in the brain tissues.
Keywords
Agrimoniae herba Hematoma; iNOS; c-Fos; MMP-9; MMP-12;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Unterberg AW, Stover J, Kress B, KieningKL. Edema and brain trauma. Neuroscience. 2004; 129 (4):1021-1029.
2 Xi G, Keep RF, Hoff JT. Pathophysiology of brain edema formation. Neurosurg Clin N Am. 2002;13:371-383.   DOI   ScienceOn
3 Ropper AH. Lateral displacement of the brain and level of consciousness in patients with an acute hemispheral mass. N Engl J Med. 1986;314 :953- 958.   DOI   ScienceOn
4 Onodera H, Kogure K, Ono Y, Igarashi K, Kiyota Y, Nagaoka A. Proto-oncogene c-fos is transiently induced in the rat cerebral cortex after forebrain ischemia. Neurosci Lett. 1989; 98:101-104.   DOI   ScienceOn
5 Heo JH, Han SW, Lee SK. Free radicals as triggers of brain edema formation after stroke. Free Radic Biol Med. 2005;39(1):51-70.   DOI   ScienceOn
6 Liu K, Mori S, Takahashi HK, Tomono Y, Wake H, Kanke T, Sato Y, Hiraga N, Adachi N, Yoshino T, Nishibori M. Anti-high mobility group box 1 monoclonal antibody ameliorates brain infarction induced by transient ischemia in rats. FA-SEB J. 2007;21(14):3904-3916.
7 Mun-Bryce S, Rosenberg GA. Matrix metalloproteinases in cerebrovascular disease. J Cereb Blood Flow Metab. 1998;18:1163-1172.   DOI
8 Yong VW, Power C, Forsyth P, Edwards DR. Metalloproteinases in biology and pathology of the nervous system. Nat Rev Neurosci. 2001;2:502-511.   DOI   ScienceOn
9 Ansari MA, Abdul HM, Joshi G, Opii WO, Butterfield DA. Epub 2008 Jul 7. Protective effect of quercetin in primary neurons against Abeta(1-42 ) : relevance to Alzheimer's disease. J Nutr Biochem. 2009 Apr;20 (4):269-75.   DOI   ScienceOn
10 Silva B, Oliveira PJ, Dias A, Malva JO. Quercetin, kaempferol and biapigenin from Hypericum perforatum are neuroprotective against excitotoxic insults. Neurotox Res. 2008 May-Jun;13 (3-4):265-79.   DOI   ScienceOn
11 Rosenberg GA, Mun-Bryce S, Wesley M, Kornfeld M. Collagenase induced intracerebral hemorrhage in rats. Stroke. 1990;21:801-807.   DOI   ScienceOn
12 Cho JY, Kim IS, Jang YH, Kim AR, Lee SR. Epub 2006 Jun 27. Protective effect of quercetin, a natural flavonoid against neuronal damage after transient global cerebral ischemia. Neurosci Lett. 2006 Sep 1;404(3):330-5.   DOI   ScienceOn
13 Jorgensen HS, Nakayama H, Raaschou HO, Olsen TS. Intracerebral hemorrhage versus infarction: stroke severity, risk factors, and prognosis. Ann Neurol. 1995;38:45-50.   DOI   ScienceOn
14 Begley DJ, Brightman MW. Structural and functional aspects of the blood-brain barrier. Prog Drug Res. 2003;61:39-78.
15 Wolburg H, Lippoldt A. Tight junctions of the blood-brain barrier: development, composition and regulation. Vascul Pharmacol. 2002; 38(6):323-337.   DOI   ScienceOn
16 Abbott NJ, Ronnback L, Hansson E. Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci. 2006;7(1):41-53.   DOI   ScienceOn
17 Feuerstein GZ, Wang X, Barone FC. The role of cytokines in the neuropathology of stroke and neurotrauma. Neuroimmunomodulation. 1998;5(3-4):143-159.   DOI   ScienceOn
18 Hsu MF, Young JH, Wang JP, Teng CM. Effect of hsien-ho-t'sao (Agrimonia pilosa) on experimental thrombosis in mice. Am J Chin Med. 1987;15(1-2):43-51.   DOI
19 Abbott NJ. Inflammatory mediators and modulation of blood-brain barrier permeability. Cell Mol Neurobiol. 2000;20:131-147.   DOI   ScienceOn
20 Vijayababu MR, Arunkumar A, Kanagaraj P, Venkataraman P, Krishnamoorthy G,Arunakaran J. Quercetin downregulates matrix metalloprotei nases 2 and 9 proteins expression in prostatic cansar cells(PC-3). Mol Cell Biochem. 2006 Jul; 287(1-2):109-16. Epub 2006 Apr 28.   DOI
21 Wang JP, Hsu MF, Teng CM. Antiplatelet effect of hsien-ho-t'sao (Agrimonia pilosa). Am J Chin Med. 1985;13(1-4):109-18.   DOI
22 Wang JP, Hsu MF, Teng CM. Antihemostatic effect of Hsien-Ho-T' sao (Agrimonia pilosa). Am J Chin Med. 1984 Summer;12(1-4):116-23.   DOI
23 Xu X, Qi X, Wang W, Chen G. Separation and determination of flavonoids in Agrimonia pilosa Ledeb. by capillary electrophoresis with electrochemical detection. J Sep Sci. 2005 May;28 (7) :647-52.   DOI   ScienceOn
24 Pan Y, Liu HX, Zhuang YL, Ding LQ, Chen LX, Qiu F. [Studies on isolation and identification of flavonoids in herbs of Agrimonia pilosa][Article in Chinese]. Zhongguo Zhong Yao Za Zhi. 2008 Dec;33 (24):2925-8.
25 Kumar A, Sehgal N, Kumar P, Padi SS, Naidu PS. Protective effect of quercetin against ICV colchicine-induced cognitive dysfunctions and oxidative damage in rats. Phytother Res. 2008 Dec;22(12):1563-9.   DOI   ScienceOn
26 Rezai-Zadeh K, Ehrhart J, Bai Y, Sanberg PR, Bickford P, Tan J, Shytle RD. Apigenin and lute- olin modulate microglial activation via inhibition of STAT1-induced CD40 expression. J Neuroinf -lammation. 2008 Sep;25;5:41.
27 Zhong Yao Cai. Neuroprotective effects of apigenin on acute transient focal cerebral ischemia-reperfusion injury in rats. 2008 Jun;31(6):870-3.
28 김영석. 중풍의 병인병리, 변증 및 치료. 한방성인병학회지. 1995;1:113-140.
29 Yao Y, Han DD, Zhang T, Yang Z. [Epub ahead of print] Quercetin improves cognitive deficits in rats with chronic cerebral ischemia and inhibits voltage-dependent sodium channels in hippocampal CA1 pyramidal neurons. Phyto -ther Res. 2009 Aug 17.
30 Ossola B, Kaariainen TM, Mannisto PT. The multiple faces of quercetin in neuroprotection. Expert Opin Drug Saf. 2009 Jul;8(4):397-409.   DOI   ScienceOn
31 Badjatia N, Rosand J. Intracerebral hemorr- hage. Neurologist. 2005;11 (6):311-324.   DOI   ScienceOn
32 Xi G, Keep RF, Hoff JT. Mechanisms of brain injury after intracerebral haemorrhage. Lancet Neurol. 2006;5(1):53-63.   DOI   ScienceOn
33 Grant JS, Elliott TR, Weaver M, Glandon GL, Raper JL, Giger JN. Social support, social problemsolving abilities, and adjustment of family caregivers of stroke survivors. Arch Phys Med Rehabil. 2006;87(3): 343-350.   DOI   ScienceOn
34 명호진, 이상복, 노재규, 윤병우, 이원용, 권오상. 최근 국내 뇌졸중의 역학적 동향에 대한 연구. 대한신 경과학회지. 1989;7:179-187.
35 Hawkins BT, Davis TP. The blood-brain barrier/neurovascular unit in health and disease. Pharmacol Rev. 2005;57(2):173-185.   DOI   ScienceOn
36 이병철, 유경호. 국내 뇌졸중의 역학. 대한의사협 회지. 2002;45:1415-1421.
37 강병수, 고운채, 김선희, 노승현, 송승준, 신민교, 안덕균, 이상인, 이영종, 이강희, 주영승. 본초학. 서울 : 영림사. 1991: 384-386.
38 Koshiura R, Miyamoto K, Ikeya Y, Taguchi H. Antitumor activity of methanol extract from roots of Agrimonia pilosa Ledeb. Jpn J Pharmacol. 1985 May;38(1):9-16.   DOI
39 Kumar A, Goyal R. Quercetin protects agai- nst acute immobilization stress-induced behavi- ors and biochemical alterations in mice. J Med Food. 2008 Sep;11(3):469-73.   DOI   ScienceOn