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http://dx.doi.org/10.6116/kjh.2010.25.4.121

Studies on the Inhibitory Effect of Berberidis Ramulus to the Liver Cancer  

Lee, Chung-Heon (Dept. of Herbology, College of Oriental Medicine, Kyungwon University)
Han, Hyo-Sang (Dept. of Herbology, College of Oriental Medicine, Kyungwon University)
Lee, Young-Jong (Dept. of Herbology, College of Oriental Medicine, Kyungwon University)
Publication Information
The Korea Journal of Herbology / v.25, no.4, 2010 , pp. 121-128 More about this Journal
Abstract
Objectives : This study purposed to research the inhibitory effect of Berberidis Ramulus on the liver cancer. Methods : A total extract of Berberidis Ramulus decoction were prepared. Through the measurement of the cell proliferation, apoptosis, morphology and cytokine level from the extracts, the influence on HepG2 cell were compared. Results : Berberidis Ramulus extract significantly inhibited the proliferation, increased the apoptosis, decreased the TGF-${\beta}$ gene expression and the K-RAS gene expression, significantly increased the level of TNF-${\alpha}$ secretion and increased the rates of IFN-${\gamma}$ secretory cells. Conclusion : It is suggested that Berberidis Ramulus extract turned out to have anti-cancer effects on HepG2 cell.
Keywords
Berberidis Ramulus; liver cancer; anti-cancer effect;
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1 김윤근, 박정순. 매자나무뿌리 알콜 추출물의 항진균 작용. 대한약리학회지. 1969;5(1):51-54.
2 조선희, 김정일. 매자나무성분이 마우스 수면 및 체온에 미치는 영향. 대한약리학회지. 1974;10(1,15):61-65.
3 이종화, 박영현, 조병헌, 김유재, 김종배, 김천숙, 차영덕, 김영석. Majarin 유도체인 majarol의 흰쥐에 있어서 혈압 및 심박동수에 대한 작용과 적출 개구리 심장에 대한 작용. 대한약리학회지. 1986;22(1,38):34-44.
4 김영균. 국내산 매자나무과 Berberis속 수목 성분 분석. 과학기술부 최종보고서. 1996:23.
5 Xu R, Dong Q, Yu Y, Zhao X, Gan X, Wu D, Lu Q, Xu X, Yu XF. Berbamine : a novel inhibitor of bcr/abl fusion gene with potent anti-leukemia activity. Leuk Res. 2006;30(1):17-23.   DOI   ScienceOn
6 Lans C, Turner N, Khan T, Brauer G, Boepple W. Ethnoveterinary medicines used for ruminants in British Columbia, Canada. J Ethnobiol Ethnomedicine. 2007;3:11.   DOI   ScienceOn
7 Tsai PL, Tsai TH. Hepatobiliary excretion of berberine. Drug Metab Dispos. 2004;32(4):405-12.   DOI   ScienceOn
8 Guseva NV, Taghiyev AF, Rokhilin OW, Cohen MB. Death receptor-induced cell death in prostate cancer. J Cell Biochem. 2004;91:70-99.   DOI   ScienceOn
9 윤정구. 종양에 대한 생체방어기전. 대한의학협회지. 1989;32(10):1073-1077.
10 Rodriguez GC, Berchuck A, Whitaker RS. Epidermal growth factor receptor expression in normal ovarian epithelium and ovarian cancer. II. Relationshop between receptor expression and response to epidermal growth factor. Am J Obstet Gynecol. 1991;164:745-750.   DOI   ScienceOn
11 Roit IM, Brostoff J, Male DK Livingstone C. Immunology. London:Gower Medical Publishing. 1989:9.6-9.13.
12 Kakimi K, Guidotti L, Koezuka Y, Chisari FV. Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J Exp Med. 2000;192:921-930.   DOI   ScienceOn
13 Gilman AG, Good LS, Gilman A. The pharmacological basis of therapeutics 6th ed. New York:Macmillan Co Inc. 1975:110-115.
14 Janbaz KH, Gilani AH. Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia. 2000;71(1):25-33.   DOI   ScienceOn
15 Shamsa F, Ahmadiani A, Khosrokhavar R. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. J Ethnopharmacol. 1999;64(2):161-6.   DOI   ScienceOn
16 金昌種. 病態生理學. 서울:癸丑文化社. 1988:72-74.
17 蘇頌 撰. 胡乃長 王致譜 輯注. 圖經本草. 九龍:龍源出版公社. 1988:315.
18 李時珍 著. 本草綱目(校點本). 北京:人民衛生出版社. 1982:1982.
19 Anis KV, Rajeshkumar NV, Kuttan R. Inhibition of chemical carcinogenesis by berberine in rats and mice. J Pharm Pharmacol. 2001;53(5):763-8.   DOI   ScienceOn
20 Fukuda K, Hibiya Y, Mutoh M, Koshiji M, Akao S, Fujiwara H. Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. J Ethnopharmacol. 1999;66(2):227-33.   DOI   ScienceOn
21 Evans VC. Multple pathways to apoptosis. Cell Biol Int. 1993;17(5):461-476.   DOI   ScienceOn
22 Pellegrion M, Ferrone S, Theofilopoulos A. Isolation of human T and B cells by rosette formation with 2-aminoethylisothiouronium bromide(AET)-treated sheep red blood cells and with monkey red blood cells. J Immunol Meth. 1976;11:273-277.   DOI   ScienceOn
23 Kishimoto T, AEG von dem Brorne et al. Leucocyte typing VI : White cell differentiation antigens. Garland publishing Inc. London. 1998.
24 Maiolini R, Masseyeff R. Asandwigh method of enzymoimmunoassay. Appication to rat and human alpha-foetoprotein. J Immunol Meth. 1975;8:223-234.   DOI   ScienceOn
25 Shi L, Nishioka WK, Th'ng J, Bradbury EM, Litchfield DW, Greenberg AH. Premature p34cdc2 activation required for apoptosis. Science. 1994;263(5150):1143-1145.   DOI
26 蘇敬 等撰. 尙志鈞 輯校. 新修本草. 安徽科學技術出版社. 1981:351.
27 國家中醫藥管理局 中華本草 編委會. 中華本草. 上海:上海科學技術出版社. 1999:(3)285-300.
28 조선민주주의 인민공화국 보건부 약전위원회. 조선민주주의 인민공화국 약전 제5판. 평양:의학과학출판사. 1996:175.
29 中華人民共和國衛生部藥典委員會 編. 中華人民共和國藥典 1977年版 1部. 北京:人民衛生出版社. 1978:23,24.
30 中華人民共和國衛生部藥典委員會 編. 中華人民共和國藥典 1990年版 1部. 北京:人民衛生出版社. 1990:부록21.
31 이창복. 대한식물도감. 서울:향문사. 1982:370.
32 이용주, 유승조, 이성규, 박동하. 매자나무과 식물의 Alkaloid 연구-왕매발톱나무의 Alkaloid 성분. 생약학회지. 1971;2(1):19-21.
33 Shin EC, Shin WC, Choi Y, Kim H, Park JH, Kim SJ. Effect of interferon-gamma on the susceptibility to Fas(CD95/APO-1)-mediated cell death in human hepatoma cells. Cancer Immunol Immunother. 2001;50:23-30.   DOI   ScienceOn
34 Mervyn J, Mary D, Gerald MC. Antitumor activity of 1-naphthol against L1210 leukemia in vivo and enrlich ascites tumor cells in vivo and in vitro. Cancer letters. 1986;33:347-354.   DOI   ScienceOn