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http://dx.doi.org/10.6116/kjh.2010.25.2.099

Anti-oxidative effects of fractionated Pulsatilla koreana $N_{AKAI}$ extracts  

Cho, Hyun-Jin (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.)
Yun, Hyun-Jeong (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.)
Yi, Hyo-Seung (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.)
Park, Sun-Dong (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.)
Publication Information
The Korea Journal of Herbology / v.25, no.2, 2010 , pp. 99-106 More about this Journal
Abstract
Objectives : This study was designed to investigate the effects of anti-oxidation of fractionated Pulsatilla koreana $N_{AKAI}$ (PK) extracts. And we examined to determine that a certain fractionated extract has the best anti-oxidative effects between the fractionated PK extracts. Methods : Anti-oxidative effects of fractionated PK extracts was measured by scavenging activities of DPPH, superoxide, nitric oxide (NO) and peroxynitrite radicals. And also scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cell was measured. After these examination, we determined a fraction that has best anti-oxidative effects. Results : Fractionated PK extracts inhibited radicals effectively. Also in RAW 264.7 cell, intracellular oxidation has inhibited by PK extracts. In these tests, ethyl acetate (EA) fraction has the best anti-oxidative effects among PK extracts. Conclusions : This results demonstrate that PK extracts exhibit anti-oxidative effects. And EA fraction has the best inhibition effects among the six fractions of PK.
Keywords
Pulsatilla koreana $N_{AKAI}$; anti-oxidation; DPPH; superoxide; nitric oxide; Peroxynitrite;
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Times Cited By KSCI : 10  (Citation Analysis)
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