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http://dx.doi.org/10.6116/kjh.2010.25.1.039

Anticoagulant Activity of Gleditsiae Spina Extract  

Yoo, Ji-Hyun (Dept. of Oriental Medicine Resources, Joongbu University)
Jung, Bung-Tea (Dept. of Oriental Medicine Resources, Joongbu University)
Kil, Gi-Jung (Dept. of Oriental Medicine Resources, Joongbu University)
Publication Information
The Korea Journal of Herbology / v.25, no.1, 2010 , pp. 39-43 More about this Journal
Abstract
Objectives : This research was investigated the anticoagulant effect of the Gleditsiae spina extract. Methods : We researched prothrombin time (PT) assay, activated partial thromboplastin time (APTT) assay in vitro and in vivo using arteriovenous (A-V) shunt rat model and shortening Rat tail bleeding time (BT). A-V shunt and BT were treated with extract of Gleditsiae spina (GS) 400 mg/kg for a week. Results : Bleeding time of Gleditsiae spina extract in vivo had a significant increase as about 1.2 times and thrombus weight of Gleditsiae spina extract had a significant reduction of thrombus weight as 26%. Gleditsiae spina extract represented an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, XI, XII in the coagulation system. Conclusions : Considering the above mentioned results, it is judged that a Gleditsiae Spina extract has a control effect of thrombus creation.
Keywords
Gleditsiae Spina; Anticoagulant; Thrombosis; Prothrombin time; Bleeding time;
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  • Reference
1 Butenas S, Ribarik N, Mann KG. Synthetic substrates for human factor VIIa and factor VII atissue factor. Biochemistry. 1993 ; 32 : 6531-8.   DOI   ScienceOn
2 Quick J. The prothrombin in haemophilia and in obstructive jaundice. Journal of Biological Chemistry. 1935.
3 Han YN, Baik SK, Kim TH, Han BH. Antithrombotic activities of saponins from Ilex pubescens. Archives of Pharmacal Research. 1987 ; 10 : 115-22.   DOI
4 Berry CN, GirardD, Lochot S, Lecoffre C. Antithrombotic actions of argatrobas in rat medels of venous, mixed and arterial thrombosis, and its effects on the tail transection bleeding time. Br J Pharmacol. 1994; 113 : 1209-14.   DOI   ScienceOn
5 Park EH, Shin MJ. Anti-inflammatory Activity of Aqueous Extract from Gleditsiae Spina. Yakhak Hoeji. 1993; 37(2) : 124-8.
6 Simon MF, Lamnt V, Lachachi H, Plantavid M, Mauco G, Chap H, Douste-blazy L. PAF Metabolism and signalling process inhibition by ginkolides. Gingkolides (Braguet P ed). JR Prous barcelona. 1988 ; 127(1).
7 Kim CH, Hah DS, Leu JD, Heo JH, Jung MH, Choe YT, Kim GS, Kim JS. Oral Toxicity Studies for 2 weeks of Gleditschia-saponin in Sprague Dawley Rats. Journal of toxicology and public health. 2002 ; 18(3) : 285-92.
8 Lee SK, Shin JI, Seo UK, Jeong JC. A Study on Activities of Antioxidant and Antimutagenecity of the Extracts from Gleditsia sinensis. Journal of Korean oriental internal medicine. 2001 ; 22(2) : 215-22.
9 Kang SY, Cho KW, Han JH, Cho NG. Effect of Gleditsiae Spina on Hep G2 cells cytotoxicity and Apoptosis and No. Journal of Korean oriental internal medicine. 1997 ; 18(1) : 48-61
10 Park YK, Kang BS, Kim JT, Park IS, Ahn SH. The Experimental Study on Antitumor effects of Gleditsia spina. Journal of Herbology. 1997 ; 12(1) : 53-66.
11 Koltai M, Tosaki A, Guillon JM, Hrosford D Braguet. PAF antogonists as potential therapeutic agents in cardiac anaphylaxia and myocardial ischaemia. Cardiovasc Drug Rev. 1989 ; 77(7).
12 Gwak HS, Chun IK. Performulation study of aspalatone, a new antithrombotic agent. Journal Appl Pharmaclo. 2000 ; 8 : 332-7.
13 Hsieh KH. Thrombin interaction with fibrin with polymerization sites. Thrombosis Research. 1997 ; 86 : 301-16.   DOI   ScienceOn
14 Oriental medical university herbal medicine the National History Compilation Committee. Clinical Traditional Herbalogy. Seoul : Younglimsa. 2006 : 305
15 Verstraeta M, Lijnen HR, Collen D. Thrombolytic agents in development. Drugs. 1995 ; 50 : 29-42.   DOI   ScienceOn
16 Akkerman JWN. Interaction between the PAF receptor and fibirinogen binding sites on human platelets. In : Gingkolide (Braquet P ed). JR Prous barcelona. 1998 ; 93(1).
17 Katira R, Chauhan A, More RS. Direct thrombin inhibitors: novel abtithrombotics on the horizon in the thrombo prophylactic management of atrial fibrillation. Postgraduate Medical Journal. 2005 ; 81 : 370-5.   DOI   ScienceOn
18 Cattaneo F, Trento F, Pescador R, Porta R, Furro L. Pharmacodynamics of the antociagulant activity (APTT) of an algal polysaccharide. Thrombosis Research. 2002 ; 105 : 455-7.   DOI   ScienceOn
19 Molina V, Arruzazabala ML, Mas CR. Synergistic effect of D-003 and aspirin on experimental thrombosis models. Prostaglandins, Leukotrienes and Essential Fatty Acids. 2003 ; 68 : 305-10.   DOI   ScienceOn