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http://dx.doi.org/10.6116/kjh.2010.25.1.001

A Chromosome Aberration Test of HMC05 on Cultured Chinese Hamster Lung Cells  

Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
Publication Information
The Korea Journal of Herbology / v.25, no.1, 2010 , pp. 1-7 More about this Journal
Abstract
Objectives : We investigated genetic toxicity of HMCO5 in relation to chromosome aberration test on Cultured Chinese Hamster Lung (CHL) in the presence and absence of S-9 mix. Methods : Experimental groups were divided into two groups: with S-9mix (+S) or without S-9 mix (-S). -S group was also divided 2 series by treatment hours (6 hr: 6-S; or 24 hr; 24-S). Each group treated with vehicle only (complete culture medium), HMCO5 (1,250, 2,500, $5,000\;{\mu}g/ml$), and cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS), respectively. Results : HMC05 did not show any aberrant metaphase. However, there were significant (p < 0.01) aberrant metaphases with CPA in S+ and with EMS in S-. Conclusions : These results indicate that HMC05 formula does not show any toxicity in chromosome aberration test.
Keywords
Genetic toxicity; HMC05; Chinese Hamster Lung cells; chromosome aberration test;
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Times Cited By KSCI : 3  (Citation Analysis)
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