Browse > Article
http://dx.doi.org/10.13066/kspm.2014.9.2.193

The Effect of Treadmill Exercise on the Expression of IL-1β in the Spinal Cord in Osteoarthritis Rats  

Park, Soo-Jin (Department of Physical Therapy, College of Science, Kyungsung University)
Choi, Young-Chul (Neuroscience Laboratory, College of Rehabilitation Science, Daegu University)
Publication Information
Journal of the Korean Society of Physical Medicine / v.9, no.2, 2014 , pp. 193-200 More about this Journal
Abstract
PURPOSE: This study examined the effects of treadmill exercise of diverse intensities on the expression of IL-$1{\beta}$ (interleukine-$1{\beta}$) in the spinal cord in osteoarthritis rats. METHODS: The authors applied treadmill exercise of diverse intensity for 4 weeks to Sprague-Dawley rats to which intra-articular injection of monosodium iodoacetate(MIA, $3mg/50{\mu}l$, diluted in saline) was applied in the right knee joint to induce osteoarthritis. The four-week exercise was not applied to the control group(CG, n=15), while exercise of applicable intensity was applied to the low-intensity exercise group(LEG, n=15), moderate-intensity exercise group (MEG, n=15), and high-intensity exercise group(HEG, n=15) for four weeks. Observations were made of expression of IL-$1{\beta}$ in the spinal cord in osteoarthritis rats using western blot analysis. RESULTS: There were significant differences(p<.05) in the comparison of expression of IL-$1{\beta}$ in the spinal cord between the four groups involved. And the LEG and MEG had reduced expression of IL-$1{\beta}$ significantly than the CG(p<.05); in particular, the MEG showed the lowest expression. On the other hand, the HEG had more elevated expression of IL-$1{\beta}$ significantly than the CG(p<.05). CONCLUSION: As a result, factors that induce neuropathic pain such as IL-$1{\beta}$ are reduced; thus, the recovery of damaged neurons is improved and neuropathic pain is reduced. Further, when prescribing exercise to treat osteoarthritis patients, exercise of moderate intensity suitable for patients' physical conditions, rather than high intensity, maximizes the effects of this therapy.
Keywords
Osteoarthritis; Neuropathic pain; IL-$1{\beta}$; Treadmill exercise;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Reid MB, Li YP. Cytokines and oxidative signalling in skeletal muscle. Acta Physiol Scand. 2001;171(3):225-32.   DOI   ScienceOn
2 Oh SD. The effect of intensity exercise on the changes T-cell and cytokines following submaximal exercise. Exercise Science. 2003;12(4):693-701.
3 Park HS, Kim JS. Clinical article : The effects of low intensity of ultrasound on monosodium iodoacetate(MIA) induced athritis in rats. Korean J Sports Med. 2009;27(1):27-32.   과학기술학회마을
4 Paek NH, Kwak SS, Lee DS et al. Characterization of peroxiredoxins in the gray matter in the spinal cord after acute immobilization stress. J Trauma Inj. 2006;19(2):105-12.
5 Rodrigues B, Figueroa DM, Mostarda CT et al. Maximal exercise test is a useful method for physical capacity and oxygen consumption determinatio in streptozotocin-diabetic rats. Cardiovasc Diabeto. 2007;6(1):38-44.   DOI   ScienceOn
6 Schuelert N, McDougall JJ. Grading of monosodium iodoacetate-induced osteoarthritis reveals a concentrationdependent sensitization of nociceptors in the knee joint of the rat. Neurosci Lett. 2009;465(2):184-8.   DOI   ScienceOn
7 Waldburger JM, Firestein GS. Regulation of peripheral inflammation by the central nervous system. Curr Rheumatol Rep. 2010;12(5):370-8.   DOI   ScienceOn
8 Whiteheada KJ, Smitha CGS, Delaney SA et al. Dynamic regulation of spinal pro-inflammatory cytokine release in the rat in vivo following peripheral nerve injury. Brain Behav Immun. 2010;24(4):569-76.   DOI   ScienceOn
9 Choi JH, Lee CH, Yoo KY et al. Colocalization of calcitonin gene-related peptide and substance P in the purkinje cells in the colchicine-treated gerbil. Lab Anim Res. 2009;25(1):15-8.   과학기술학회마을
10 Nissalo S, Hukkanen M, Imai S et al. Neuropeptides in Experimental and Degenerative Arthritis. Ann N Y Acad Sci. 2002;966(1):384-99.   DOI   ScienceOn
11 Baek KY. The effect of different exercise intensity on the blood pressure and inflammatory markers change in pre-hypertension leveled male. Graduate School of Chonnam National University. Doctor's thesis. 2008.
12 Choi JK. The Effects of high intensity exercise and DA-9601 administration on antioxidant enzyme activities and TNF-$\alpha$, IL-$1\beta$ mRNA expression in rats. Graduate School of Kyungpook National University. Master's thesis. 2010.
13 Constandil L, Hernandez A, Pelissier T et al. Effect of interleukin-$1\beta$ on spinal cord nociceptive transmission of normal and monoarthritic rats after disruption of glial function. Arthritis Res Ther. 2009;11(4):105-13.   DOI   ScienceOn
14 Fernihough J, Gentry G, Malcangio M et al. Pain related behaviour in two models of osteoarthritis in the rat knee. Pain. 2004;112(1-2):83-93.   DOI   ScienceOn
15 Fiorentino PM, Tallents RH, Miller JN et al. Spinal interleukin-$1\beta$ in a mouse model of arthritis and joint pain. Arthritis Rheum. 2008;58(10):3100-9.   DOI   ScienceOn
16 Im HJ, Kim JS, Li X et al. Alteration of sensory neurons and spinal response to an experimental osteoarthritis pain model. Arthritis Rheum. 2010;62(10):2995-3005.   DOI   ScienceOn
17 Han DY. mRNA expression on Neuropeptide(NPY) to exercise intensity and recovery time. Graduate School of Dankook University. Doctor's thesis. 2006.
18 Orita S, Ishikawa T, Miyagi M et al. Pain-related sensory innervation in monoiodoacetate-induced osteoarthritis in rat knees that gradually develops neuronal injury in addition to inflammatory pain. BMC Musculoskelet Disord. 2011;12(1):134-45.   DOI
19 Kim KY. The Changes of Cytokine Levels in The Central and Peripheral Nerves in Sciatic Nerve Injured Rats. Graduate School of Pusan National University. Doctor's thesis. 2005.
20 Inglis JJ, Nissim A, Lees DM et al. The differential contribution of tumour necrosis factor to thermal and mechanical hyperalgesia during chronic inflammation. Arthritis Res Ther. 2005;7(4):807-16.   DOI   ScienceOn
21 Kim DH, Hong SH. A proteomic approach for quantitative analysis of calcitonin gene-related peptides in the cerebrospinal fluid obtained from a rat model of chronic neuropathic pain. Korean J Pain. 2008;21(2):112-8.   과학기술학회마을   DOI   ScienceOn
22 Kim WH. The effects of treadmill exercise on blood glucose and expression of UCP-2 of brown adipose tissue and TNF-$\alpha$ of soleus muscle in obese zucker rats. Graduate School of Chonnam National University. Doctor's thesis. 2010.
23 Lee HL. Expression of cytokines and their receptors in rat dorsal root ganglia and spinal cord following chronic constriction injury of the scitic nerve. Graduate School of Kyungpook National University. Master's thesiss. 2004.
24 Helmak IC, Mikkelsen UR, Borglum J et al. Exercise increase interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial. Arthritis Res Ther. 2010;12(4):126-36.   DOI   ScienceOn
25 Lee JY. Expression of apoptosis-related proteins in muscle fiber types I&II of aged rats depending on the exercise intensity. Graduate School of Dankook University. Doctor's thesis. 2009.
26 McGaraughty S, Chu KL, Perner RJ et al. TRPA1 modulation of spontaneous and mechanically evoked firing of spinal neurons in uninjured, osteoarthritic, and inflamed rats. Mol Pain. 2010;6(1):14-24.   DOI   ScienceOn
27 Narita M, Shimamuraa M, Imaia S et al. Role of interleukin-$1\beta$ and tumor necrosis factor-$\alpha$-dependent expression of cyclooxygenase-2 mRNA in thermal hyperalgesia induced by chronic inflammation in mice. Neuroscience. 2008;152(2):477-86.   DOI   ScienceOn
28 Kim SY. Expression of JAK-STAT mRNAs in rat dorsal roots ganglia and spinal cord following peripheral nerve injuries. Graduate School of Kyungpook National University. Doctor's thesis. 2004.
29 Byun KS. Effect of exercise intensity on GLUT-4 expression of skeletal muscle and insulin resistance in type 1 diabetic rats. Graduate School of Hannam University. Doctor's thesis. 2010.