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http://dx.doi.org/10.13066/kspm.2014.9.2.133

Comparison of Current Density on Iontophoretic Transdermal Delivery of Methylene Blue in Rat Skin  

Lee, Jae-Hyoung (Department of Physical Therapy, ElectroTherapy Research Laboratory for Tissue Growth and Repair, Wonkwang Health Science University)
Kim, Jin-Kyung (Department of Cosmetics, Wonkwang Health Science University)
Kwon, Pil-Seung (Department of Clinical Laboratory Science, Wonkwang Health Science University)
Jekal, Seung-Joo (Department of Clinical Laboratory Science, Wonkwang Health Science University)
Publication Information
Journal of the Korean Society of Physical Medicine / v.9, no.2, 2014 , pp. 133-140 More about this Journal
Abstract
PURPOSE: The purpose of this study was to compare the effect of current density on penetration depth, tissue concentration and transdermal transport of methylene blue(MB) by iontophoretic transdermal delivery. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into 1 mA($0.11mA/cm^2$), 2 mA($0.22mA/cm^2$), 4 mA($0.44mA/cm^2$), and 8 mA($0.89mA/cm^2$) groups. These rats were exposed to anodic iontophoresis of 1% MB using a direct current for 15 minutes. The penetration depth were measured using light microscopy from cryosections of skin tissue. The tissue concentration and transdermal transport were measured using biochemical analysis from target skin tissues. The data were analyzed with one-way analysis of variance. RESULTS: The significant differences in the penetration depth, tissue concentration and transdermal transport were detected among the groups(p<.001). Post hoc comparisons of the penetration depth, tissue concentration and transdermal transport of he 2 mA, 4 mA, and 8 mA iontophoresis groups were greater than in the 1 mA iontophoresis group(p<.05). There was no significant difference, however, among 2 mA, 4 mA, and 8 mA iontophoresis group. CONCLUSION: There was no difference in the efficiency of iontophoresis from 2 mA($0.22mA/cm^2$) to 8 mA($0.89mA/cm^2$). Higher current density can cause skin injury and discomfort sensation. In general, $0.5mA/cm^2$ is proposed to be the maximum iontophoretic current which should be used on human. The appropriate current amplitude should be selected by considering the safety current density and the depth of the target tissue.
Keywords
Iontophoresis; Penetration depth; Tissue concentration; Transdermal transport; Methylene blue;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Holbrook KA, Odland GF. Regional differences in the thickness (cell layers) of the human stratum corneum: an ultrastructural analysis. J Invest Dermatol. 1974;62(4): 415-22.   DOI   ScienceOn
2 Barry BW. Novel mechanisms and devices to enable successful transdermal drug delivery. Eur J Pharm Sci. 2001;14(2):101-14.   DOI   ScienceOn
3 Dixit N, Bali V, Baboota S, et al. Iontophoresis - an approach for controlled drug delivery: a review. Curr Drug Deliv. 2007;4(1):1-10.
4 Glass JM, Stephen RL, Jacobson SC. The quantity and distribution of radiolabeled dexamethasone delivered to tissue by iontophoresis. Int J Dermatol. 1980;19(9):519-25.   DOI
5 Kanikkannan N. Iontophoresis-based transdermal delivery systems. BioDrugs. 2002;16(5):339-47.   DOI   ScienceOn
6 Lane ME. Skin penetration enhancers. Int J Pharm. 201315; 447(1-2):12-21.   DOI   ScienceOn
7 Deshpande S, Lahoti S, Shah R, et al. Iontophoresis: A physical approach to transdermal drug delivery system. Research J Pharm Tech. 2012;5(2):175-80.
8 Cazares-Delgadillo J, Balaguer-Fernandez C, Calatayud-Pascual A, et al. Transdermal iontophoresis of dexamethasone sodium phosphate in vitro and in vivo: effect of experimental parameters and skin type on drug stability and transport kinetics. Eur J Pharm Biopharm. 2010;75(2):173-8.   DOI   ScienceOn
9 Chelly JE, Grass J, Houseman TW, et al. The safety and efficacy of a fentanyl patient-controlled transdermal system for acute postoperative analgesia: a multicenter, placebo-controlled trial. Anesth Analg. 2004;98(2):427-33.
10 Costello CT, Jeske AH. Iontophoresis: applications in transdermal medication delivery. Phys Ther. 1995;75(6):554-63.   DOI
11 Barry BW. Drug delivery routes in skin: a novel approach. Adv Drug Deliv Rev. 2002;54 Suppl 1:S31-40.   DOI   ScienceOn
12 Benson HA. Transdermal drug delivery: penetration enhancement techniques. Curr Drug Deliv. 2005;2(1):23-33.   DOI   ScienceOn
13 Wang Y, Thakur R, Fan Q, et al. Transdermal iontophoresis: combination strategies to improve transdermal iontophoretic drug delivery. Eur J Pharm Biopharm. 2005;60(2):179-91.   DOI   ScienceOn
14 Xu Q, Ibrahim SA, Higuchi WI, et al. Ion-exchange membrane assisted transdermal iontophoretic delivery of salicylate and acyclovir. Int J Pharm. 2009;369(1-2):105-13.   DOI   ScienceOn
15 Zempsky WT, Sullivan J, Paulson DM, et al. Evaluation of a low-dose lidocaine iontophoresis system for topical anesthesia in adults and children: a randomized, controlled trial. Clin Ther. 2004;26(7):1110-9.   DOI   ScienceOn
16 Vemulapalli V, Banga AK, Friden PM. Optimization of iontophoretic parameters for the transdermal delivery of methotrexate. Drug Deliv. 2008;15(7):437-42.   DOI   ScienceOn
17 Singh P, Maibach HI. Iontophoresis in drug delivery: basic principles and applications. Crit Rev Ther Drug Carrier Syst. 1994;11(2-3):161-213.
18 Wallace MS, Ridgeway B, Jun E, et al. Topical delivery of lidocaine in healthy volunteers by electroporation, electroincorporation, or iontophoresis: an evaluation of skin anesthesia. Reg Anesth Pain Med. 2001;26(3):229-38.
19 Singh P, Roberts MS. Iontophoretic transdermal delivery of salicylic acid and lidocaine to local subcutaneous structures. J Pharm Sci. 1993;82(2):127-31.   DOI
20 Sintov AC, Brandys-Sitton R. Facilitated skin penetration of lidocaine: combination of a short-term iontophoresis and microemulsion formulation. Int J Pharm. 200619;316(1-2):58-67.   DOI   ScienceOn
21 Mathy FX, Lombry C, Verbeeck RK, et al. Study of the percutaneous penetration of flurbiprofen by cutaneous and subcutaneous microdialysis after iontophoretic delivery in rat. J Pharm Sci. 2005 Jan;94(1):144-52.   DOI   ScienceOn
22 Lee Jae-Hyoung, Choi Eun-Young. Iontophoresis enhances transdermal delivery of methylene blue in rat skin (I): Effect of current application duration. J Kor Soc Phys Ther 2011;23(6):77-84.   과학기술학회마을
23 Li GL, Van Steeg TJ, Putter H, et al. Cutaneous side-effects of transdermal iontophoresis with and without surfactant pretreatment: a single-blinded, randomized controlled trial. Br J Dermatol. 2005;153(2):404-12.   DOI   ScienceOn
24 Lilge L, O'Carroll C, Wilson BC. A solubilization technique for photosensitizer quantification in ex vivo tissue samples. J Photochem Photobiol B. 1997;39(3): 229-35.   DOI   ScienceOn
25 Prasad R, Anand S, Koul V. Biophysical assessment of DC iontophoresis and current density on transdermal permeation of methotrexate. Int J Pharm Investig. 2011;1(4):234-9.   DOI   ScienceOn
26 Riviere JE, Monteiro-Riviere NA, Inman AO. Determination of lidocaine concentrations in skin after transdermal iontophoresis: effects of vasoactive drugs. Pharm Res. 1992;9(2):211-4.   DOI   ScienceOn
27 Siddoju S, Sachdeva V, Friden PM, et al. Iontophoretic delivery of acyclovir: intradermal drug monitoring using microdialysis and quantification by skin extraction. PDA J Pharm Sci Technol. 2011 Sep-Oct;65(5): 432-44.   DOI   ScienceOn
28 Hage M, Akatani M, Kikuchi J, et al. Transdermal iontophoretic delivery of insulin using a photoetched microdevice. J Control Rel. 1997;43(2-3):139-49.   DOI   ScienceOn
29 Brown MB, Martin GP, Jones SA, et al. Dermal and transdermal drug delivery systems: current and future prospects. Drug Deliv. 2006;13(3):175-87.   DOI   ScienceOn