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Suppressive Effects of Various Antioxidants on Melamine-induced Oxidative DNA Damage in Human Lymphocytes  

Park, Seul-Ki (Department of Medical Biotechnology, SoonChunHyang University)
Lee, Mi-Young (Department of Medical Biotechnology, SoonChunHyang University)
Publication Information
Molecular & Cellular Toxicology / v.5, no.3, 2009 , pp. 243-249 More about this Journal
Abstract
Melamine, which is used to produce melamine resin for various industrial applications, has a high nitrogen content by mass. For this reason, it has been illegally added to foods to increase their apparent protein content. In the present investigation, melamine-induced oxidative damage of human lymphocyte DNA was evaluated by Comet assay. The in vitro oxidative DNA damage caused by melamine increased in a dose-dependent manner. This DNA damage was significantly inhibited by treatment with ascorbate. Moreover, the traditional Korean medicinal herb, named Acanthopanax, red ginseng and green tea markedly reduced the DNA damage. Various edible plant extracts also inhibited melamine-induced oxidative DNA damage in vitro. Melamine enhanced intracellular ROS generation, and this effect was suppressed by treatment with various antioxidants.
Keywords
Melamine; Comet assay; Oxidative DNA damage; Reactive oxygen species (ROS); Antioxidant;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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