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The Gene Expression Profile of LPS-stimulated Microglial Cells  

Sohn, Sung-Hwa (BK21 Oriental Medical Science Center, KyungHee University)
Ko, Eun-Jung (BK21 Oriental Medical Science Center, KyungHee University)
Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, KyungHee University)
Kim, Yang-Seok (BK21 Oriental Medical Science Center, KyungHee University)
Shin, Min-Kyu (BK21 Oriental Medical Science Center, KyungHee University)
Hong, Moo-Chang (BK21 Oriental Medical Science Center, KyungHee University)
Bae, Hyun-Su (BK21 Oriental Medical Science Center, KyungHee University)
Publication Information
Molecular & Cellular Toxicology / v.5, no.2, 2009 , pp. 147-152 More about this Journal
Abstract
This study was conducted to evaluate the inflammatory mechanisms of LPS-stimulated BV-2 microglial cells. The inflammation mechanism was evaluated in BV-2 cells with or without LPS treated using the Affymetrix microarray analysis system. The microarray analysis revealed that B cell receptor signaling pathway, cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, MAPK signaling pathway, Neuro-active ligand-receptor interaction, TLR signaling path-way, and T cell receptor signaling pathway-related genes were up-regulated in LPS stimulated BV-2 cells. Selected genes were validated using real time RTPCR. These results can help an effective therapeutic approach to alleviating the progression of neuro-in-flammatory diseases.
Keywords
Gene expression profile; Inflammatory effects; LPS; Microglial cell;
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