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Evaluation of the Genotoxicity of Decursin and Decursinol Angelate Produced by Angelica gigas Nakai  

Kim, Kang-Min (Department of Smart Foods and Drugs, Inje University)
Kim, Tae-Ho (Department of Smart Foods and Drugs, Inje University)
Park, Yun-Jung (Department of Smart Foods and Drugs, Inje University)
Kim, Ik-Hwan (College of Life Sciences and Biotechnology, Korea University)
Kang, Jae-Seon (Department of Pharmacy, Kyungsung University)
Publication Information
Molecular & Cellular Toxicology / v.5, no.1, 2009 , pp. 83-87 More about this Journal
Abstract
In this study, we assessed the stability and toxicological safety of Angelica gigas Nakai (A. gigas Nakai) extract, which is comprised of decursin and decursinol angelate (D/DA). D/DA was tested for mutagenicity using Ames Salmonella tester strains (TA102, TA1535, and TA1537) with or without metabolic activation (S9 mix). No increase in the number of revertants was observed in response to any of the doses tested (1.25, 12.5, 125, and $1,250{\mu}/mLg$). In addition, a chromosome aberration test was conducted in the Chinese hamster lung (CHL) cell line. To accomplish this, cells were treated with D/DA (3.28, 13.12, 52.46, and $209.84{\mu}g/mL$) or with Mitomycin C ($0.1{\mu}/mLg$) as a positive control in the case of no metabolic activation or benzo(a)pyrene ($20{\mu}g/mL$) in the case of metabolic activation. No significant increase in chromosome aberrations was observed in response to treatment with any of these concentrations, regardless of activation of the metabolic system. According to these results, we concluded that D/DA did not induce bacterial reverse mutation or clastogenicity in vitro in the range of concentrations evaluated in these experiments.
Keywords
Mutagenicity; Ames test; Chromosome aberration; Angelica gigas Nakai; Decursin; Decursinol angelate;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
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