Browse > Article

Comparison of the Effects of Nano-silver Antibacterial Coatings and Silver Ions on Zebrafish Embryogenesis  

Yeo, Min-Kyeong (Department of Environmental Science and Engineering, KyungHee University)
Yoon, Jae-Won (Department of Environmental Science and Engineering, KyungHee University)
Publication Information
Molecular & Cellular Toxicology / v.5, no.1, 2009 , pp. 23-31 More about this Journal
Abstract
To compare the effects of nanometer-sized silver ions and support materials (nano-silver coating material, NM-silver) and silver ions, we exposed zebrafish embryos to both types of nano-silver ions and compared the acute responses during embryogenesis. The amount of silver in the NM-silver (17.16%) was greater than that in the silver ion (4.56%). Both of these materials have different atomic compositions. The silver ion-exposed groups (10 and 20 ppt) showed lower survival rates than the NM-silver-exposed groups (10 and 20 ppt). NM-silver penetrated the skin and blood tube of zebrafish larvae as aggregated particles, whereas, silver ions penetrated the organelles, nucleus and yolk in a spread-out pattern. Micro-array analysis of RNA from zebrafish larvae (72 hours post-fertilization) that were treated with either NM-silver or silver ions, showed alteration in expression of the BMP, activin, TGF-$\beta$, and $GSK3{\beta}$ genes pathway. Additionally, $GSK3{\beta}$ gene pathway for apoptosis that was related with left-right asymmetry. Gene expression changes in the NM-silver or silver ions-treated zebrafish embryo led to phenotypic changes in the hatched larvae, reflecting increased apoptosis and incomplete formation of an axis.
Keywords
Nano-silver antibacterial coatings; Silver ion; Biological toxicity; $GSK3{\beta}$; TGF-$\beta$; Zebrafish;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 10  (Related Records In Web of Science)
연도 인용수 순위
1 Harper, T. Nano Korea. http://www.nanotechweb. org (2003)
2 Sondi, I. & Salopek-Sondi, B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interf Sci 275: 177-182 (2004)   DOI   ScienceOn
3 Berger, T. J., Spadaro J. A., Chapin S. E. & Becker R. O. Electrically generated silver ions: quantitative effects on bacterial and mammalian cells. Antimicrob Agents Ch 9: 357-358 (1976)   DOI   ScienceOn
4 Woodgett, J. R. Judging a protein by more than its name: GSK-3. Sci Signal 12:1-11(2001)   DOI   ScienceOn
5 Möller, W., Hofer, T., Ziesenis, A., Karg, E. & Heyder, J. Ultrafine particles cause cytoskeletal dysfunctions in macrophages. Toxicol Appl Pharm 182:197-207 (2002)   DOI   ScienceOn
6 Jung, W. K. et al. Antifungal activity of the silver ion against contaminated fabric. Mycoses 50:265-269 (2007)   DOI   ScienceOn
7 Jia, S., Ren, Z., Li, X., Zheng, Y. & Meng, A. Smad2 and Smad3 are required for mesendoderm induction by transforming growth factor-/nodal signals in Zebrafish. J Biol Chem 283:2418-2426 (2008)   DOI   ScienceOn
8 Yeo, M. K. & Park, S. W. Exposing zebrafish to silver nanoparticles during caudal fin regeneration disrupts caudal fin growth and p53 signaling. Mol Cell Toxicol 4: 311-317(2008)   과학기술학회마을
9 Yeo, M. K & Kang, K. Effects of nanometer sized silver materials on biological toxicity during zebrafish embryogenesis. Bull Korean Chem Soc 29:1179-1184 (2008)   과학기술학회마을   DOI   ScienceOn
10 Brooker, R. J. & Slayman, C. W. Effects of Mg2+ ions on the plasma membrane [H+]-ATPase of Neurospora crassa. II. Kinetic studies. J Biol Chem 258:8833-8838 (1983)
11 Woodgett, J. R. cDNA cloning and properties of glycogen synthase kinase-3. Methods Enzymol 200:564-577 (1991)   DOI
12 Lee, H. J., Yeo, S. Y. & Jeong, S. H. Antibacterial effect of nanosized silver colloidal solution on textile fabrics. J Mater Sci 38:2199-2204 (2003)   DOI   ScienceOn
13 Black, C. B., Huang, H. W. & Cowan, J. A. Biological coordination chemistry of magnesium, sodium, and potassium ions. Protein and nucleotide binding sites. Coord Chem Rev 135:165-202 (1994)   DOI   ScienceOn
14 Radi, A. A. R. & Matkovics, B. Effects of metal ions on the antioxidant enzyme activities, protein contents and lipid peroxidation of carp tissues. Comp Biochem Physiol C 90:69-72 (1988)   DOI   ScienceOn
15 Roche, H. & Boge, G. Effects of Cu, Zn and Cr salts on antioxidant enzyme activities in vitro of red blood cells of a marine fish. Dicentrarchus labrax. Toxicol In Vitro 7:623-629 (1993)   DOI   ScienceOn
16 Hamouda, T. et al. A novel surfactant nanoemulsion with broad-spectrum sporicidal activity against Bacillus species. J Infect D 180:2096-2126 (1999)   DOI   ScienceOn
17 Lambert, A. L., Mangum, J. B., Delorme, M. P. & Everitt, J. I. Ultrafine carbon black particles enhance respiratory syncytial virus-induced airway reactivity, pulmonary inflammation, and chemokine expression. Soc Toxicol 72:339-346 (2003)   DOI   ScienceOn
18 Ramsdell, A. F. Left-right asymmetry and congenital cardiac defects: getting to the heart of the matter in vertebrate left right axis determination. Dev Biol 288:1-20 (2005)   DOI   ScienceOn
19 Rederstorff, M., Krol, A. & Lescure, A. Understanding the importance of selenium and selenoproteins in muscle function. Cell Mol Life Sci 63:52-59 (2006)   DOI
20 Lee, H. C. et al. Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebra-fish embryo. BMC Dev Biol 7:1-15 (2007)   DOI
21 Kimmel, W., Ballard, S., Ullman, B. K. & Schilling, T. Stages of embryonic development of the zebrafish. Dev Dynam 203: 253-310 (1995)   DOI   ScienceOn
22 Hossain, Z. & Huq, F. Studies on the interaction between Ag+ and DNA. J Inorg Biochem 91:398-404 (2002)   DOI   ScienceOn
23 Nagaso, H., Suzuki, A., Tada, M. & Ueno, N. Dual specificity of activin type II receptor ActRIIb in dorsoventral patterning during zebrafish embryogenesis. Develop Growth Differ 41:119-133 (1999)   DOI   ScienceOn
24 Renwick, L. C., Donaldson, K. & Clouter, A. Impairment of alveolar macrophage phagocytosis by ultrafine partic. Toxicol Appl Pharm 172:119-127 (2001)   DOI   ScienceOn