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Chlorella vulgaris Has Preventive Effect on Cadmium Induced Liver Damage in Rats  

Shim, Jae-Young (Department of Food and Nutrition, College of Human Ecology, Hanyang University)
Om, Ae-Son (Department of Food and Nutrition, College of Human Ecology, Hanyang University)
Publication Information
Molecular & Cellular Toxicology / v.4, no.2, 2008 , pp. 138-143 More about this Journal
Abstract
We investigated if Chlorella vulgaris (CV) has protective effects on cadmium (Cd) induced liver damage in male Sprague-Dawley (SD) rats. Forty rats, aged 5 weeks old and weighed 90-110g, were divided into a control (with Cd free water), 50 ppm of $CdCl_2$ in drinking water treated groups (Chlorella 0% diet group (Cd/CV0%), Chlorella 5% diet group (Cd/CV5%) or Chlorella 10% diet group (Cd/CV10%). All the rats had freely access to water and diet for 8 weeks. The results show that body weight gain and relative liver weight had significantly lower in Cd/CV0%-treated group than in Cd/CV-treated groups. Hepatic Cd contents showed significantly less by feeding CV (P<0.05). Cd/CV0%-treated rats had significantly (P<0.05) higher hepatic T-MTs, and Cd-MTs concentrations, compared to Cd/CV5% or Cd/CV10% treated rats. The MT I/II mRNA was expressed in the liver of all experimental rats. Its expression was more increased in Cd/CV5%- or Cd/CV10%-treated rats, compared to control and Cd-treated rats. Thus, this study suggested that CV would have a protective effect on Cd-treated liver injury by the reduction of Cd concentrations and stimulation of Cd-MT binds in the liver. However, more studies are needed to identify the proper mechanism of CV and liver toxicity.
Keywords
Chlorella vulgaris; Rats; Cadmium; Liver toxicity; Metallothionein;
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1 Kagi, J. H. & Kojima, Y. Chemistry and biochemistry of metallothionein. Experientia Suppl 52:25-61 (1987)
2 Morita, K., Matsueda, T., Lida, T. & Hasegawa, T. Chlorella accelerates dioxin excretion in rats. J Nutr 129:1731-1736 (1999)   DOI
3 Hendler, S. S., Fleming, T., Deutsch, M. & Wyble, C. Supplement monographs chlorella. In PDR for Nutritional Supplements. 1ed, pp. 86-88. Medical Economics Company, Inc., NJ. (2001)
4 Ryu, D. Y. et al. Dietary iron regulates intestinal cadmium absorption through iron transporters in rats. Toxicology Letter 152:19-25 (2004)   DOI   ScienceOn
5 Alvarez, S. M., Gomez, N. N. & Luis, S. Morphorogical changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium. Toxicology Letters 153:265-276 (2004)
6 Roels, H., Lauwerys, R., Buchet, J. P. & Bernard, A. Environmental exposure to cadmium and renal function of aged women in three areas of Belgium. Environ Res 24:117-130 (1981)   DOI   ScienceOn
7 Elinder, C. G., Kjellstrom, T., Linnman, L. & Pershagen, G. Urinary excretion of cadmium and zinc among persons from Sweden. Environ Res 15:473-484 (1978)   DOI   ScienceOn
8 Kim, M. J., Hong, J. H. & Rhee, S. J. Effects of vitamine E on cadmium accumulation and excretion in chronic cadmium poisoned rats. Kor J Nutr 36:691-698 (2003)
9 Schroeder, H. A., Nason, A. P., Prior, R .E., Reed, J. B. & Haessler, W. T. Influence of cadmium in renal ischemic hypertension in rats. Am J Physiol 214:469-474 (1968)
10 Cherian, M. G. et al. Role of metallothioneins in carcinogenesis. Toxicol Appl Pharmacol 126:1-5 (1994)   DOI   ScienceOn
11 Han, S. H., Shin, M. K. & Chung, Y. H. Effects of the omija extract on the metabolism and renal cadmium contents in cadmium administered rats. J Korean Soc Food Sci 31:1102-1106 (2002)   과학기술학회마을   DOI
12 Konishi, F., Tanaka, K., Himeno, K., Taniguchi, K. & Nomoto, K. Antitumor effect induced by a hot water extract of Chlorella vulgaris (CE): resistance to Meth -A tumor growth mediated by CE-induced polymorphonuclear leukocytes. Cancer Immunol Immunother 19:73-78 (1985)
13 Peters, J. M., Thomas, D., Falk, H., Oberdorster, G. & Smith, T. J. Contribution of metals to respiratory cancer. Environ Health Perspect 70:71-83 (1986)   DOI
14 Friberg, L., Piscator, M., Nordberg, G. F. & Kjellstrom, T. Cadmium in the environment, 2nd ed., CRC Press, Cleveland (1974)
15 Hasegawa, T. et al. Hot water extracts of Chlorella vulgaris reduce opportunistic infection with Listeria monocytogenes in C57BL/6 mice infected with LPBM5 murine leukemia viruses. Int J Immunopharmacol 17:505-512 (1995)   DOI   ScienceOn
16 Om, A. S., Chung, K. W. & Chung, H. S. Effect of cadmium accumulation on renal tissues in broilers. Bull Environ Contam Toxicol 68:297-301 (2002)   DOI
17 Hwang, Y. K., Choi, H. J., Nam, M., Yoo, J. D. & Kim, Y. H. Effects of chlorella on metallothionein synthesis and binding capacity of cadmium in cadmium poisoned rat liver and kidney. J Exp Biomed Sci 12:23-27 (2006)
18 Goering, P. L. & Klaassen, C. D. Tolerance to cadmium- induced toxicity depends on presynthesized metallothionein in liver. J Toxicol Environ Health 14:803-812 (1984)   DOI   ScienceOn
19 Lauwerys, R. R., Buchet, J. P., Roels, H. A., Brouwers, J. & Stanescu, D. Epidemiological survey of workers exposed to cadmium. Arch Environ Health 28:145-148 (1974)   DOI   ScienceOn
20 Mitsumori, K. et al. Relationship between the development of hepato-renal toxicity and cadmium accumulation in rats given minimum to large amounts of cadmium chloride in the long-term: preliminary study. Arch Toxicol 72:545-552 (1998)   DOI
21 Waalkes, M. P., Anver, M. R. & Diwan, B. A. Chronic toxic and carcinogenic effects of oral cadmium in the Noble (NBL/Cr) rat: induction of neoplastic and proliferative lesions of the adrenal, kidney, prostate, and testes. J Toxicol Environ Health 29:199-214 (1999)
22 Probst, G. S., Bousqest, W. F. & Miya, T. S. Kinetics of cadmium-induced hepatic and renal metallothionein synthesis in the mouse. Toxicol Appl Pharmacol 39:51-60 (1977)   DOI   ScienceOn
23 Ellis, K. J., Cohn, S. H. & Smith, T. H. Cadmium inhalation exposure estimates: Their significance with respect to kidney and liver cadmium burden. J Toxicol Environ Health 15:173-187 (1985)   DOI   ScienceOn
24 Miranda, M. S., Sato, S. & Mancini-Filho, J. Antixoidant activity of the microalage Chlorella vulgaris cultured on special conditions. Boll Chim Farm 140: 165-168 (2001)
25 Reeves, P. G., Chaney, R. L., Simmons, R. W. & Cherian, M. G. Metallothionein induction is not involved in cadmium accumulation in the duodenum of mice and rats fed diets containing high-cadmium rice or sunflower kernels and a marginal supply of zinc, iron, and calcium. J Nutr 135:99-108 (2005)
26 Oteiza, P. L., Olin, K. L., Fraga, C. G. & Keen, C. L. Oxidant defense systems in testes from zinc-deficient rats. Proc Soc Exp Biol Med 213:85-91 (1996)
27 Kaplan, D., Christiaen, D. & Arad, S. Chelating properties of extracellular polysaccharides from Chlorella spp. App and Environ Micro 53:2953-2956 (1987)
28 Yoshiki, S., Yanagisawa, T. & Kimura, M. Bone and kidney leisons in experimetal cadmium intoxification. Arch Environ Health 30:559-562 (1975)   DOI   ScienceOn
29 WHO. Environmental Health Criteria 134, Cadmium. IPCS, Gerneva (1992)