Browse > Article

Gene Expression Analysis of Lung Injury in Rats Induced by Exposure to MMA-SS Welding Fume for 30 Days  

Oh, Jung-Hwa (Toxicogenomics Team, Korea Institute of Toxicology)
Park, Han-Jin (Toxicogenomics Team, Korea Institute of Toxicology)
Heo, Sun-Hee (Toxicogenomics Team, Korea Institute of Toxicology)
Yang, Mi-Jin (Inhalation toxicology Team, Korea Institute of Toxicology)
Yang, Young-Su (Inhalation toxicology Team, Korea Institute of Toxicology)
Song, Chang-Woo (Inhalation toxicology Team, Korea Institute of Toxicology)
Yoon, Seok-Joo (Toxicogenomics Team, Korea Institute of Toxicology)
Publication Information
Molecular & Cellular Toxicology / v.3, no.4, 2007 , pp. 306-313 More about this Journal
Abstract
The welding fume has been implicated as a causal agent in respiratory disease such as pneumoconiosis. The molecular mechanism by which welding fume induces toxicity in the lung is still unknown, but studies have focused on histological structure and indirect approach measuring the pulmonary damage markers. In the present study, gene expression profiles were analyzed in the lung of rats exposed by manual metal-arc stainless-steel (MMA-SS) welding fume for 30 days using Affymetrix GeneChip$^{(R)}$. Totally, 379 genes were identified as being either up- or down-regulated over 2-fold changes (P<0.01) in the lung of low- or high-dose group and were analyzed by using hierarchical clustering. We focused on genes involved in immune/inflammation responses were differentially regulated during lung injury induced by welding fume exposure. The information of these deregulated genes may contribute in elucidation of the inflammation mechanism during lung injury such as lung fibrosis.
Keywords
Welding fume; Long term exposure; Inflammation; Gene expression; Lung fibrosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
연도 인용수 순위
1 Yu, I. J. et al. Recovery from manual metal arc-stainless steel welding-fume exposure induced lung fibrosis in Sprague-Dawley rats. Toxicol Lett 143:247-259 (2003)   DOI   ScienceOn
2 Hedenstedt, A. et al. Mutagenicity of fume particles from stainless steel welding. Scand J Work Environ Health 3:203-211 (1977)   DOI   ScienceOn
3 Barnes, P. J., Chung, K. F. & Page, C. P. Inflammatory mediators of asthma: an update. Pharmacol 50: 515-596 (1998)
4 Kolb, M., Margetts, P. J., Sime, P. J. & Gauldie, J. Proteoglycans decorin and biglycan differentially modulate TGF-beta-mediated fibrotic responses in the lung. Am J Physiol Lung Cell Mol Physiol 280: L1327-L1334 (2001)
5 Baran, C. P. et al. Important roles for macrophage colony-stimulating factor, CC chemokine ligand 2, and mononuclear phagocytes in the pathogenesis of pulmonary fibrosis. Am J Respir Crit Care Med 176: 78-89 (2007)   DOI   ScienceOn
6 Cavanagh, L. L. et al. Proliferation in monocyte-derived dendritic cell cultures is caused by progenitor cells capable of myeloid differentiation. Blood 92:1598-1607 (1998)
7 Cavanagh, L. L., Saal, R. J., Grimmett, K. L. & Thomas, R. Idiopathic pulmonary fibrosis related to endothelial injury and antiendothelial cell antibodies. Hum Immunol 67:284-297 (2006)   DOI   ScienceOn
8 Powell, C. L. et al. Phenotypic anchoring of acetaminophen- induced oxidative stress with gene expression profiles in rat liver. Toxicol Sci 93:213-222 (2006)   DOI   ScienceOn
9 Chung, H. et al. Comprehensive analysis of differential gene expression profiles on carbon tetrachlorideinduced rat liver injury and regeneration. Toxicol Appl Pharmacol 206:27-42 (2004)
10 Oda, H. et al. Microarray analysis of the genes induced by tetracycline-regulated expression of NDRF/ NeuroD2 in P19 cells. Biochem Biophys Res Commun 335:458-468 (2005)   DOI   ScienceOn
11 Waring, J. F. et al. Clustering of hepatotoxins based on mechanism of toxicity using gene expression profiles. Toxicol Appl Pharmacol 175:28-42 (2001)   DOI   ScienceOn
12 Antonini, J. M., Krishna, G. G. & Brain, J. D. Responses to welding fumes: lung injury, inflammation, and the release of tumor necrosis factor-alpha and interleukin-1 beta. Exp Lung Res 23:205-227 (1997)   DOI
13 Wetmore, B. A. & Merrick, B. A. Toxicoproteomics: proteomics applied to toxicology and pathology. Toxicol Pathol 32:619-642 (2004)   DOI   ScienceOn
14 Kalliomäki, P. L. et al. Kinetics of the metal components of intratracheally instilled stainless steel welding fume suspensions in rats. Br J Ind Med 43:112-119 (1986)
15 Furuie H. et al. Altered accessory cell function of alveolar macrophages: a possible mechanism for induction of Th2 secretory profile in idiopathic pulmonary fibrosis. Eur Respir J 10:787-794 (1997)
16 Massague, J. TGF-beta signal transduction. Annu Rev Biochem 67:753-791 (1998)   DOI   ScienceOn
17 Maxild, J. et al. Mutagenicity of fume particles from metal arc welding on stainless steel in the Salmonella /microsome test. Mutat Res 56:235-243 (1978)   DOI   ScienceOn
18 Furuie, H., Yamasaki, H., Suga, M. & Ando, M. Effect of short-term stainless steel welding fume inhalation exposure on lung inflammation, injury, and defense responses in rats. Toxicol Appl Pharmacol 223:234-245 (2007)   DOI   ScienceOn
19 Bringardner, B. D., Baran, C. P., Eubank, T. D. & Marsh, C. B. The Role of inflammation in the pathogenesis of idiopathic pulmonary fibrosis. Antioxid Redox Signal 10:1-15 (2007)
20 Taylor, M. D. et al. Effects of welding fumes of differing composition and solubility on free radical production and acute lung injury and inflammation in rats. Toxicol Sci 75:181-191 (2003)   DOI   ScienceOn
21 Waters, M. D. & Fostel, J. M. Toxicogenomics and systems toxicology: aims and prospects. Nat Rev Genet 5:936-948 (2004)   DOI   ScienceOn
22 Yu, I. J. et al. Lung fibrosis in Sprague-Dawley rats, induced by exposure to manual metal arc-stainless steel welding fumes. Toxicol Sci 63:99-106 (2001)   DOI   ScienceOn
23 Danielsen, T. E., Langard, S., Andersen, A. & Knudsen, O. Incidence of cancer among welders of mild steel and other shipyard workers. Br J Ind Med 50: 1097-1103 (1993)
24 Moulin, J. J. et al. A mortality study among mild steel and stainless steel welders. Br J Ind Med 50:234-243 (1993)
25 Oh, J. H. et al. Gene expression profiling of early renal toxicity induced by gentamicin in mice. Mol Cell Toxicol 2:185-192 (2006)   과학기술학회마을
26 Antonini, J. M., Lawryk, N. J., Murthy, G. G. & Brain, J. D. Effect of welding fume solubility on lung macrophage viability and function in vitro. J Toxicol Environ Health A 58:343-363 (1999)   DOI
27 Rim, K. T. et al. Gene-expression profiling of human mononuclear cells from welders using cDNA microarray. J Toxicol Environ Health A 70:1264-1277 (2007)   DOI   ScienceOn
28 Uemitsu, N. et al. Inhalation toxicity study of welding fumes: effect of single or repeated exposure on the lungs of rats. Toxicology 30:75-92 (1984)   DOI   ScienceOn
29 Yu, I. J. et al. Recovery from welding-fume-exposure-induced lung fibrosis and pulmonary function changes in sprague dawley rats. Toxicol Sci 82:608-613 (2004)   DOI   ScienceOn
30 Rim, K. T. et al. Gene-expression profiling using suppression-subtractive hybridization and cDNA microarray in rat mononuclear cells in response to welding-fume exposure. Toxicol Ind Health 20:77-88 (2004)   DOI
31 Tuschl, H., Weber, E. & Kovac, R. Investigations on immune parameters in welders. J Appl Toxicol 17: 377-383 (1997)   DOI   ScienceOn
32 Morgan, W. K. C. On welding, wheezing, and whimsy. Am Ind Hyg Assoc J 50:59-69 (1989)   DOI