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Toxicoproteomic Analysis of Differentially Expressed Proteins in Rat Liver by DEHP  

Son, Bu-Soon (Department of Environmental Health Scinece, SoonChunHyang University)
Seong, Ah-Reum (Department of Biotechnology, SoonChunHyang University)
Park, Seul-Ki (Department of Biotechnology, SoonChunHyang University)
Kim, Wan-Jong (Department of Life Science, SoonChunHyang University)
Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology)
Lee, Mi-Young (Department of Biotechnology, SoonChunHyang University)
Publication Information
Molecular & Cellular Toxicology / v.3, no.4, 2007 , pp. 299-305 More about this Journal
Abstract
The endocrine disrupting chemical, di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in polyvinyl chloride products ubiquitous in our daily lives. DEHP has potentially adverse effects on the liver, kidney, lung, heart, reproductive organs and endocrine systems. Many toxicological data on the DEHP toxicity have been stated, but complete protein profiles have not yet been reported. In this study, DEHP-induced oxidative DNA damage in rat lymphocyte was evaluated by Comet assay (single-cell gel electrophoresis) for the first time. Moreover, DEHP-induced protein profile alterations were examined in rat liver by using toxicoproteomic tools. 34 protein spots in the liver were identified to be significantly deregulated by DEHP on the 2-dimensional gel. Among them, 20 spots were up-regulated and 14 spots down-regulated by DEHP.
Keywords
DEHP; Rat liver; Toxicoproteomics;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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