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Evaluation of the Genetic Toxicity of Synthetic Chemical (XVII) -In vitro Mouse Lymphoma Assay and In vitro Supravital Micronucleus Assay with 1, 2-Dichlorobenzene  

Kim, Youn-Jung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology)
Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology)
Publication Information
Molecular & Cellular Toxicology / v.3, no.2, 2007 , pp. 113-118 More about this Journal
Abstract
Chlorobenzenes due to their acute toxicity and the capability of bioaccumulating are of great health and environmental concern. Especially, 1, 2-dichlorobenzene (CAS No. 95-50-1) is used for organic synthesis, dye manufacture, as a solvent and for other applications in chemical industry. Adverse effects of 1, 2-dichlorobenzene includes increases in liver and kidney weights and hepatotoxicity. In this study, we evaluated the genetic toxicity of 1, 2-dichlorobenzene with more advanced methods, in vitro mouse lymphoma assay $tk^{+/-}$ gene assay (MLA) and in vitro mouse supravital micronucleus (MN) assay. 1, 2-Dichlorobenzene appeared the significantly positive results and the induction of large mutant colonies only in the presence of metabolic activation system with MLA. But in vitro testing of 1, 2-dichlorobenzene yielded negative results with supravital MN assay. These results suggest that 1, 2-dichlorobenzene may play a mutagen rather than clastogen in vitro mammalian system.
Keywords
1, 2-Dichlorobenzene; In vitro Mouse Lymphoma Assay; In vitro supravital micronucleus assay; Genotoxicity;
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  • Reference
1 US Department of Commerce. US exports, schedule E commodity by country (FT410/December 1980), Bureau of Census, Washington DC, pp. 2-74(1981)
2 Kumagai, S. & Matsunaga, I. Identification of urinary metabolites of human subjects exposed to o-dichlorobenzene. Int. Arch. Occup. Environ. Health 67:207- 209 (1995)
3 Kirkland, D. J. Genetic toxicology testing requirements: official and unofficial views from Europe. Environ. Mol. Mutagen. 2:8-14(1993).
4 Tennant, R. W. et al. Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays. Science 236:933-941 (1987)   DOI
5 Perocco, P., Bolognesi, S. & Alberghini, W. Toxic activity of seventeen industrial solvents and halogenated compounds on human lymphocytes cultured in vitro. Toxicol. Lett. 16:69-75 (1983)   DOI   ScienceOn
6 Reustle, J. A. & Scribner, H. E. o-Dichlorobenzene; Myelotoxicity and cytogenetic study in rats. Report of the Toxicology Department, Rohm and Haas Company, Spring House, Pennsylvania. EPA/OTS Doc. No. 878212182, 1-71 (1979)
7 Amaral, O. C., Otero, R. & Grimalt, J. O. Volatile and semi-volatile organochlorine compounds in tap and riverine waters in the area of influence of a chlorinated organic solvent factory. Water Res. 30(8): 1876-1884(1996)   DOI   ScienceOn
8 Applegate, M. L. et al. Molecular dissection of mutations at the heterozygous thymidine kinase locus in mouse lymphoma cells. Proc. Natl. Acad. Sci. USA 87:51-55 (1990)
9 Sofuni, T. Japanese guidelines for mutagenicity testing. Environ. Mol. Mutagen. 21:2-7 (1993)   DOI   ScienceOn
10 Myhr, B. C. & Caspary, W. J. Chemical mutagenesis at the thymidine kinase locus in L5178Y mouse lymphoma cells: results for 31 coded compounds in the National Toxicology Program. Environ. Mol. Mutagen. 18:51-83 (1991)   DOI   ScienceOn
11 Clements, J. Gene mutation assays in mammalian cells, In S. O'Hare and C. K. Atterwill (Ed.), Methods in Molecular Biology, Vol. 43: in vitro Toxicity Testing Protocols. Humana Press Inc. Totowa, NJ. 277- 286 (1990)
12 BUA Report No. 53, VCH, September (1990)
13 Zapata-Gayon, C., Zapata-Gayon, N. & Gonzalez- Angulo, A. Clastogenic chromosomal aberrations in 26 individuals accidentally exposed to ortho dichlorobenzene vapors in the National Medical Center in Mexico City. Arch. Environ. Health 37:231-235 (1982)   DOI   ScienceOn
14 Health Protection Branch Genotoxicity Committee. The assessment of mutagenicity: health protection branch mutagenicity guidelines. Environ. Mol. Mutagen. 21:15-37 (1993)   DOI   ScienceOn
15 Matsui, S., Yamamoto, R. & Yamada, H. The Bacillus subtilis/microsome ret-assay for the detection of DNA damaging substances which may occur in chlorinated and ozonated waters. Wat. Sci. Tech. 21:875- 887 (1989)   DOI
16 Vogel, E. W. & Nivard, M. J. Performance of 181 chemicals in a Drosophila assay predominantly monitoring interchromosomal mitotic recombination. Mutagenesis 8:57-81 (1993)   DOI   ScienceOn
17 Martí, I., Lloret, R., Martín-Alonso, J. & Ventura, F. Determination of chlorinated toluenes in raw and treated water samples from the Llobregat river by closed loop stripping analysis and gas chromatography- mass spectrometry detection. J. Chromatogr. 1077(1):68-73 (2005)   DOI   ScienceOn
18 Huybrechts, T., Dewulf, J. & Van Langenhove, H. Priority volatile organic compounds in surface waters of the southern North Sea. Environ. Pollut. 133(2): 255-264(2005).   DOI   ScienceOn
19 ECETOC. 'Risk assessment in marine environments'. Technical Report No. 82, Brussels, pp. 141, ISSN- 0773-8072-82 (2001)
20 Auletta, A. E., Dearfield, K. L. & Cimino, M. C. Mutagenicity test schemes and guidelines: US EPA office of pollution prevention and office of pesticide programs. Environ. Mol. Mutagen. 21:38-45 (1993)   DOI   ScienceOn
21 Shelby, M. D., Erexson, G. L., Hook, G. J. & Tice, R. R. Evaluation of a three-exposure mouse bone marrow micronucleus protocol: results with 49 chemicals. Environ. Mol. Mutagen. 21:160-179 (1993)   DOI   ScienceOn
22 Kitchin, K. T., Brown, J. L. & Kulkarni, A. P. Predictive assay for rodent carcinogenicity using in vitro biochemical parameters: operational characteristics and complementarity. Mutation Res. 266:253-272 (1992)   DOI
23 Robinson, W. D. et al. Statistical evaluation of bacterial/ mammalian fluctuation tests, in Statistical Evaluation of Mutagenicity Test Data (Kirkland, D. J., ed.). Cambridge University Press, Cambridge, UK, 102-140 (1990)
24 Loveday, K. S., Anderson, B. E., Resnick, M. A. & Zeiger, E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. V: Results with 46 chemicals. Environ. Mol. Mutagen. 16:272-303 (1990)   DOI   ScienceOn
25 Ames, B. N., Durston, W. E., Yamasaki, E. & Lee, F. D. Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. USA 70: 2281-2285 (1973)
26 OECD. SIDS Initial Assessment Report For SIAM 13 1, 2-DICHLOROBENZENE Bern, Switzerland, 6-9 November. UNEP PUBLICATIONS (2001).
27 Heidrich, S. et al. Regionally contaminated aquiferstoxicological relevance and remediation options (Bitterfield case study). Toxicology 205(3):143-155 (2004)   DOI   ScienceOn
28 Hayashi, M. et al. Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe. Mutation Res. 307:245-251 (1994)   DOI
29 Hayashi, M. et al. The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides. Mutation Res. 245:245-249 (1990)   DOI   ScienceOn
30 Mohtashamipur, E., Triebel, R., Straeter, H. & Norpoth, K. The bone marrow clastogenicity of eight halogenated benzenes in male NMRI mice. Mutagenesis 2:111-113 (1987)   DOI   ScienceOn