Browse > Article

Growth of Budding Yeasts under Optical Trap  

Im, Kang-Bin (Department of Physics, Hanyang University)
Kim, Hyun-Ik (Department of Physics, Hanyang University)
Kim, Soo-Ki (Department of Microbiology, Institute of Basic Medical Sciences, Yonsei University, Wonju College of Medicine)
Kim, Chul-Geun (Department of Life Science, Hanyang University)
Oh, Cha-Hwan (Department of Physics, Hanyang University)
Song, Seok-Ho (Department of Physics, Hanyang University)
Kim, Pill-Soo (Department of Physics, Hanyang University)
Publication Information
Molecular & Cellular Toxicology / v.3, no.1, 2007 , pp. 19-22 More about this Journal
Abstract
Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.
Keywords
Optical trap; Interferometric optical tweezers; Budding yeast cell;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Ashkin, A. Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime. Biophys. J. 61:569 (1992)
2 Wang, M. D. et al. Force and velocity measured for single molecules of RNA polymerase. Science 282: 902-907 (1998)   DOI   ScienceOn
3 Eriksen, R. L., Daria, V. R. & Glukstad, J. Fully dynamic multiple-beam optical tweezers. Opt. Express 10: 597-602 (2002)   DOI
4 Neuman, K. C. et al. Characterization of photodamage to Escherichia coli in optical traps. Biophys. J. 77: 2856-2863 (1999)   DOI   ScienceOn
5 Dai, J. & Sheetz, M. P. Mechanism properties of neuronal growth cone membrane studied by tether formation with laser optical tweezers. Biophys. J. 68:988 (1995)
6 Simmons, R. M., Finer, J. T., Chu, S. & Spudich, J. A. Quantitative measurements of force and displacement using an optical trap. Biophys. J. 70:1813 (1996)
7 Ashkin, A, Dziedzic, J. M., Bjorkholm, J. E. & Chu, S. Observation of a single-beam gradient force optical trap for dielectric particles. Opt. Lett. 11:288-290 (1986)   DOI
8 Burns, M. M., Fournier, J. M. & Golovchenko, J. A. Optical matter: Crystallization and binding in intense optical fields. Science 249:749-754 (1990)   DOI   ScienceOn
9 Tyska, M. J & Warshaw, D. M. The myosin power stroke. Cell. Motil. Cytoskeleton 51:1 (2002)
10 Korda, P., Spalding, G. C., Dufresne, E. R. & Grier, D.G. Nanofabrication of holographic optical tweezers. Rev. Sci. Instrum. 73:1956-1957 (2002)   DOI   ScienceOn
11 Pralle, A. et al. Three- dimensional high-resolution particle tracking for optical tweezers by forward scattered light. Micros. Res. Tech. 44:378 (1999)
12 Im, K. B. et al. Budding and growth of optically trapped yeasts. CLEO 2002, Lasers and Electro-Optics, Technical Digest 554-555 (2002)
13 Kuo, S. C. & Sheetz, M. P. Force of single kinesin molecules measured by optical tweezers. Science 260:232 (1993)
14 Macdonald, M. P. et al. Creation and manipulation of three dimensional optically trapped structures. Science 296:1101-1103 (2002)   DOI   ScienceOn
15 Dao, M., Lim, C. T. & Suresh, S. Mechanics of the human red blood cell deformed by optical tweezers. J. Mech. Phys. Solids 51:2259 (2003)   DOI   ScienceOn
16 Guo, H.L. et al. Mechanical properties of breast cancer cell membrane studied with optical tweezers. Chin. Phys. Lett. 21:2543 (2004)   DOI   ScienceOn