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Identification of Immune Responsive Genes on Benzene, Toluene and o-Xylene in Jurkat Cells Using 35 k Human Oligomicroarray  

Sarma, Sailendra Nath (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology)
Kim, Youn-Jung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology)
Jeon, Hee-Kyung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology)
Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology)
Publication Information
Molecular & Cellular Toxicology / v.2, no.4, 2006 , pp. 229-235 More about this Journal
Abstract
Volatile organic compounds (VOCs) are a major component of urban air pollution. It is documented that low exposure levels of VOCs induce alterations in immune reactivity resulting in a subsequent higher risk for the development of allergic reactivity and asthma. Despite these facts, there are few reports on the affected primary target and the underlying effective causal mechanisms. So in this study, to better understand the risk of BTX (benzene, toluene and o-xylene) which are the major VOCs and to identify novel biomarkers on immune response to these VOCs exposure in human T lymphocytes, we performed the toxicogenomic study by analyzing of gene expression profiles using 35 k human oligo-microarray. BTX generated specific gene expression patterns in Jurkat cell line. By clustering analysis, we identified some genes as potential markers on immuno-modulating effects of BTX. Four genes of these, HLA-DOA, ITGB2, HMGA2 and 5TAT4 were the most significantly affected by BTX exposure. Thus, this study suggests that these differentially expressed immune genes may play an important role in the pathogenesis on BTX exposure and have significant potential as novel biomarkers of exposure, susceptibility and response to BTC.
Keywords
Benzene; Toluene; o-xylene; BTX; Volatile organic compounds; Immune responsive gene; 35 k human oligomicroarray; Jurkat cell;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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