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Gene Expression Profiles Related with TCDD-Induced Hepatotoxicity  

Ryu, Yeon-Mi (Department of Biochemistry & Molecular biology, Korea University Medical School)
Kim, Ki-Nam (Department of Biochemistry & Molecular biology, Korea University Medical School)
Kim, Yu-Ri (Department of Biochemistry & Molecular biology, Korea University Medical School)
Sohn, Sung-Hwa (Department of Biochemistry & Molecular biology, Korea University Medical School)
Seo, Sang-Hui (Department of Biochemistry & Molecular biology, Korea University Medical School)
Lee, Seung-Ho (Department of Biochemistry & Molecular biology, Korea University Medical School)
Kim, Hye-Won (Department of Biochemistry & Molecular biology, Korea University Medical School)
Won, Nam-Hee (Department of Pathology, Korea University Medical School)
Kim, Meyoung-Kon (Department of Biochemistry & Molecular biology, Korea University Medical School)
Publication Information
Molecular & Cellular Toxicology / v.1, no.3, 2005 , pp. 164-171 More about this Journal
Abstract
Toxicological studies have an object of detecting adverse effects of a chemical on an organism based on observed toxicity marker (i.e., serum biochemical markers and chemical-specific gene expression) or phenotypic outcome. To date, most toxicogenomic studies concentrated on hepatic toxicity. cDNA microarray analysis enable discrimination of the responses in animals exposed to different classes of hepatotoxicants. In an effort to further characterize the mechanisms of 2, 3, 7, 8,-Tetrachlorodibenzo-p-dioxin (TCDD or dioxin)-mediated toxicity, comprehensive temporal-responsive microarray analyses were performed on hepatic tissue from Sprague-Dawley rats treated with TCDD. Hepatic gene expression profiles were monitored using custom DNA chip containing 490 cDNA clones related with toxicology. Gene expression analysis identified 26 features which exhibited a significant change. In this study, we observed that the genes related with oxidative stress in rats exposed to Dioxin, such as CYPIIA3 and glutathione S-transferase, were up-regulated at 24hr after exposure. In this study, we carried out to discover novel evidence for previously unknown gene expression patterns related to mechanism of hepatic toxicity in rats exposed to dioxin, and to elucidate the effects of dioxin on the gene expression after exposure to dioxin.
Keywords
2, 3, 7, 8,-Tetrachlorodibenzo-p-dioxin (TCDD or dioxin); hepatotoxicity; cDNA microarray; gene expression;
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1 Bulera, S.J. et al. RNA expression in the early characterization of hepatotoxicants in Wistar rats by high-density DNA microarrays. Hepatology 33(5), 1239-1258 (2001)   DOI   ScienceOn
2 Fisher, M.T., Nagarkatti, M. & Nagarkatti, P.S. Combined screening of thymocytes using apoptosisspecific cDNA array and promoter analysis yields novel gene targets mediating TCDD-induced toxicity. Toxicol. Sci. 78, 116-24 (2004)   DOI
3 Watanabe, H. et al. Comparative uterine gene expression analysis after dioxin and estradiol administration. J. Mol. Endocrinol. 33(3), 763-71 (2004)   DOI   ScienceOn
4 Davis, B.J., McCurdy, E.A., Miller, B.D., Lucier, G.W. & Tritscher, A.M. Ovarian tumors in rats induced by chronic 2, 3, 7, 8-tetrachlorodibenzo-pdioxin treatment. Cancer Res. 60, 5414-5419 (2000)
5 Tanaka, T.S. et al. Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. Proc. Natl. Acad. Sci. 97, 9127-9132 (2000)   DOI   ScienceOn
6 Puga, A., Maier, A. & Medvedovic, M. The transcriptional signature of dioxin in human hepatoma HepG2 cells. Biochem. Pharmacol. 60, 1129-1142 (2000)   DOI   ScienceOn
7 Arpiainen, S., Raffalli-Mathieu, F., Lang, M.A., Pelkonen, O. & Hakkola, J. Regulation of the Cyp2a5 gene involves an aryl hydrocarbon receptor-dependent pathway. Mol. Pharmacol. 67(4), 1325-1333 (2005)   DOI   ScienceOn
8 Nishimura, N. et al. Induction of metallothionein in the livers of female Sprague-Dawley rats treated with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin. Life Sci. 69(11), 1291-1303 (2001)   DOI   ScienceOn
9 Matsumura, F. On the significance of the role of cellular stress response reactions in the toxic actions of dioxin. Biochem. Pharmacol. 66(4), 527-540 (2003)   DOI   ScienceOn
10 Hori, M., Kondo, H., Ariyoshi, N., Yamada, H. & Oguri K. Species-specific alteration of hepatic glucose 6-phosphate dehydrogenase activity with coplanar polychlorinated biphenyl: evidence for an Ah-receptorlinked mechanism. Chemosphere. 35(5), 951-958 (1996)   DOI   ScienceOn
11 Vrana, K.E., Freeman, W.M. & Aschner, M. Use of Microarray Technologies in Toxicology Research. Neurotoxicology 24(3), 321-332 (2003)   DOI   ScienceOn
12 Diez-Fernandez, C., Sanz, N. & Cascales, M. Changes in glucose-6-phosphate dehydrogenase and malic enzyme gene expression in acute hepatic injury induced by thioacetamide. Biochem. Pharmacol. 51(9), 1159-1163 (1996)   DOI   ScienceOn
13 Pons, N., Pipino, S. & De Matteis F. Interaction of polyhalogenated compounds of appropriate configuration with mammalian or bacterial CYP enzymes. Increased bilirubin and uroporphyrinogen oxidation in vitro. Biochem. Pharmacol. 66(3), 405-414 (2003)   DOI   ScienceOn
14 Minami, K. et al. Relationship between hepatic gene expression profiles and hepatotoxicity in five typical hepatotoxicant-administered rats. Toxicol. Sci. 87(1), 296-305 (2005)   DOI   ScienceOn
15 Couture, L.A., Abbott, B.D. & Birnbaum, L.S. A critical review of the developmental toxicity and teratogenicity of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin: recent advances toward understanding the mechanism. Teratology 42, 619-627 (1990)   DOI   ScienceOn
16 Volz, D.C., Bencic, D.C., Hinton, D.E., Law, J.M. & Kullman, S.W. 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes). Toxicol. Sci. 85, 572-584 (2005)   DOI   ScienceOn
17 McMillian, M. et al. A gene expression signature for oxidant stress/reactive metabolites in rat liver. Biochem. Pharmacol. 68(11), 2249-2261 (2004)   DOI   ScienceOn
18 Kletzien, R.F., Harris, P.K. & Foellmi, L.A. Glucose- 6-phosphate dehydrogenase: a “housekeeping” enzyme subject to tissue-specific regulation by hormones, nutrients, and oxidant stress. FASEB J. 8(2), 174-181 (1994)   DOI
19 Vawter, M.P. et al. Application of cDNA microarrays to examine gene expression differences in schizophrenia. Brain. Res. Bull. 55, 641-650 (2001)   DOI   ScienceOn
20 Ishizuka, M., Yonemoto, J., Zaha, H., Tohyama, C. & Sone, H. Perinatal exposure to low doses of 2, 3, 7, 8- tetrachlorodibenzo-p-dioxin alters sex-dependent expression of hepatic CYP2C11. J. Biochem. Mol. Toxicol. 17, 278-85 (2003)   DOI   ScienceOn
21 Vezina C.M., Walker N.J. & Olson J.R. Subchronic exposure to TCDD, PeCDF, PCB126, and PCB153: effect on hepatic gene expression. Environ. Health Perspect. 112, 1636-1644 (2004)   DOI   ScienceOn
22 Burczynski, M.E. et al. Toxicogenomics-based discrimination of toxic mechanism in HepG2 human hepatoma cells. Toxicol. Sci. 58(2), 399-415 (2000)   DOI
23 Tan Z., Huang M., Puga A. & Xia Y. A critical role for MAP kinases in the control of Ah receptor complex activity. Toxicol. Sci. 82, 80-7 (2004)   DOI
24 Bartosiewicz, M., Penn, S. & Buckpitt, A. Applications of gene arrays in environmental toxicology: fingerprints of gene regulation associated with cadmium chloride, benzo(a)pyrene, and trichloroethylene. Environ. Health Perspect. 109(1), 71-74 (2001)   DOI   ScienceOn
25 Waring, J.F. et al. Clustering of hepatotoxins based on mechanism of toxicity using gene expression profiles. Toxicol. Appl. Pharmacol. 175(1), 28-42 (2001)   DOI   ScienceOn
26 Korkalainen, M., Tuomisto, J. & Pohjanvirta, R. Primary structure and inducibility by 2, 3, 7, 8-tetraclrodibenzo- p-dioxin (TCDD) of aryl hydrocarbon receptor repressor in a TCDD-sensitive and a TCDDresistant rat strain. Biochem. Biophys. Res. Commun. 315, 123-131 (2004)   DOI   ScienceOn
27 Bagchi, D. et al. Comparative effects of TCDD, endrin, naphthalene and chromium (VI) on oxidative stress and tissue damage in the liver and brain tissues of mice. Toxicology 14, 73-82 (2002)
28 Rivera, S.P., Saarikoski, S.T. & Hankinson, O. Identification of a novel dioxin-inducible cytochrome P450. Mol. Pharmacol. 61(2), 255-259 (2002)   DOI   ScienceOn
29 Slezak, B.P., Diliberto, J.J. & Birnbaum, L.S. 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin-mediated oxidative stress in CYP1A2 knockout (CYP1A2-/-) mice. Biochem. Biophys. Res. Commun. 264(2), 376-379 (1999)   DOI   ScienceOn
30 Mimura, J. & Fujii-Kuriyama, Y. Functional role of AhR in the expression of toxic effects by TCDD. Biochim. Biophys. Acta. 1619, 263-268 (2003)   DOI   ScienceOn
31 Eisen, M.B., Spellman, P.T., Brown, P.O. & Botstein, D. Cluster analysis and display of genome-wide expression patterns. Proc. Natl. Acad. Sci. 95, 14863- 14868 (1998)   DOI   ScienceOn
32 Bauman, J.W., Liu, J., Liu, Y.P. & Klaasse, C.D. Increase in metallothionein produced by chemicals that induce oxidative stress. Toxicol. Appl. Pharmacol. 110(2), 347-354 (1991)   DOI   ScienceOn
33 Fletcher, N. et al. 2, 3, 7, 8-Tetrachlorodibenzo-pdioxin (TCDD) alters the mRNA expression of critical genes associated with cholesterol metabolism, bile acid biosynthesis, and bile transport in rat liver: A microarray study. Toxicol Appl. Pharmacol. 22, 207(1):1-24 (2005)   DOI   ScienceOn
34 Boverhof, D.R. et al. Temporal and dose-dependent hepatic gene expression patterns in mice provide new insights into TCDD-Mediated hepatotoxicity. Toxicol. Sci. 85, 1048-1063 (2005)   DOI   ScienceOn
35 Boverhof, D.R. et al. Temporal and dose-dependent hepatic gene expression patterns in mice provide new insights into TCDD-Mediated hepatotoxicity. Toxicol. Sci. 85, 1048-1063 (2005)   DOI   ScienceOn
36 Schmidt, C.K. et al. 2, 3, 7, 8-tetrachlorodibenzo-pdioxin (TCDD) alters the endogenous metabolism of all-trans-retinoic acid in the rat. Arch. Toxicol. 77(7), 371-383 (2003)   DOI   ScienceOn
37 Barouki, R. & Morel, Y. Repression of cytochrome P450 1A1 gene expression by oxidative stress: mechanisms and biological implications. Biochem. Pharmacol. 61(5), 511-516 (2001)   DOI   ScienceOn
38 Adachi, J., Mori, Y., Matsui, S. & Matsuda, T. Comparison of gene expression patterns between 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and a natural arylhydrocarbon receptor ligand, indirubin. Toxicol. Sci. 80, 161-169 (2004)   DOI
39 Heijne, W.H. et al. Profiles of metabolites and gene expression in rats with chemically induced hepatic necrosis. Toxicol. Pathol. 33(4), 425-33 (2005)   DOI   ScienceOn
40 Mandal, P.K. Dioxin: a review of its environmental effects and its aryl hydrocarbon receptor biology. J. Comp. Physiol. 175, 221-230 (2005)   DOI