Browse > Article

Anti Inflammatory Effect of Low Level Laser Irradiation on the LPS-stimulated Murine Immunocytes  

Jin, Dan (Department of Microbiology, Institute of Basic Medical Science, Wonju Colleg eof Medicine, Yonsei University)
Lee, Jong-Young (Department of Radiation Oncology Wonju College of Medicine, Yonsei University)
Cho, Hyun-Chul (Department of Microbiology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
Kim, Soo-Ki (Department of Microbiology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
Publication Information
Molecular & Cellular Toxicology / v.1, no.2, 2005 , pp. 124-129 More about this Journal
Abstract
Pro-inflammatory cytokines, such as tumor necrosis factor $(TNF)-{\alpha}$, interleukin-12 (IL-12) and interleukin $(IL-1)-{\beta}$, play a key role in causing inflammatory diseases, which are rheumatoid arthritis, Crohn's disease and sepsis. Accumulating evidences suggest that low level laser irradiation (LLLI) may have an anti-inflammatory action. However, there are few data regarding down regulation of Th1 immune response by using the diod typed laser emitting device for human patients. As a fundamental step in order to address this issue, we investigated immunological impact of the low level laser irradiation (10 mw laser diode with a wavelength of 630 nm) on expression of pro-inflammatory cytokines in murine immunocytes (splenocytes and peritoneal macrophages) in vitro. The LLLI on lipopolysaccharide (LPS 100 ng/ml)-stimulated murine splenocytes and macrophages, clearly down regulated mRNA expression of $TNF-{\alpha}$ and IL-12 in dose-dependent manner. In addition, LLLI significantly inhibits the NO production in the LPS-stimulated murine macrophages. This data suggests that LLLI (wavelength of 630 nm) may exert an anti-inflammatory action via modulation of pro-inflammatory cytokine and NO production pathway.
Keywords
low level laser irradiation(LLLI); pro-inflammatory cytokine; nitric oxide (NO); RT-PCR;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Walsh, L.J. The current status of low level laser therapy in dentistry. Part 1. Soft tissue applications. Aust Dent. J. 42, 247-254 (1997)   DOI   PUBMED   ScienceOn
2 Karu, T.I., Pyatibrat, L.V., Kalendo, G.S. & Esenaliev, R.O. Effects of monochromatic low-intensity light and laser irradiation on adhesion of HeLa cells in vitro. Lasers Surg. Med. 18, 171-177 (1996)   DOI   ScienceOn
3 Hemvani N, C.D., & Bhagwanani NS. Effect of helium-neon laser on cultured human macrophages. Laser Therapy. 10, 159-164 (1998)   DOI   ScienceOn
4 Agaiby A, Ghali L. & M., D. Laser modulation of Tlymphocyte proliferation in vitro. Laser Therapy. 10, 153-158 (1998)   DOI   ScienceOn
5 Yu, H.S., Chang, K.L., Yu, C.L., Chen, J.W. & Chen, G.S. Low-energy helium-neon laser irradiation stimulates interleukin-1 alpha and interleukin-8 release from cultured human keratinocytes. J. Invest. Dermatol. 107, 593-596 (1996)   DOI   ScienceOn
6 Forstermann, U., Nakane, M., Tracey, W.R. & Pollock, J.S. Isoforms of nitric oxide synthase: functions in the cardiovascular system. Eur. Heart J. 14 Suppl I, 10- 15 (1993)
7 Karu, T., Andreichuk, T. & Ryabykh, T. Changes in oxidative metabolism of murine spleen following laser and superluminous diode (660-950 nm) irradiation: effects of cellular composition and radiation parameters. Lasers Surg. Med. 13, 453-462 (1993)   DOI   ScienceOn
8 Lubart, R., Friedmann, H., Sinyakov, M., Cohen, N. & Breitbart, H. Changes in calcium transport in mammalian sperm mitochondria and plasma membranes caused by 780 nm irradiation. Lasers Surg. Med. 21, 493-499 (1997)   DOI   ScienceOn
9 Hrnjak, M., Kuljic-Kapulica, N., Budisin, A. & Giser, A. Stimulatory effect of low-power density He-Ne laser radiation on human fibroblasts in vitro. Vojnosanit. Pregl. 52, 539-546 (1995)
10 Ohshima, H. & Bartsch, H. Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat. Res. 305, 253-264 (1994)   DOI   ScienceOn
11 Karu, T.I., Pyatibrat, L.V. & Ryabykh, T.P. Nonmonotonic behavior of the dose dependence of the radiation effect on cells in vitro exposed to pulsed laser radiation at lambda = 820 nm. Lasers Surg. Med. 21, 485-492 (1997)   DOI   ScienceOn
12 Hobbs, A.J., Higgs, A. & Moncada, S. Inhibition of nitric oxide synthase as a potential therapeutic target. Annu. Rev. Pharmacol. Toxicol. 39, 191-220 (1999)   DOI   ScienceOn
13 Funk, J.O., Kruse, A., Neustock, P. & Kirchner, H. Helium-neon laser irradiation induces effects on cytokine production at the protein and the mRNA level. Exp. Dermatol. 2, 75-83 (1993)   DOI   ScienceOn
14 Funk, J.O., Kruse, A. & Kirchner, H. Cytokine production after helium-neon laser irradiation in cultures of human peripheral blood mononuclear cells. J. Photochem. Photobiol. B 16, 347-355 (1992)   DOI   ScienceOn
15 Mordan, L.J., Burnett, T.S., Zhang, L.X., Tom, J. & Cooney, R.V. Inhibitors of endogenous nitrogen oxide formation block the promotion of neoplastic transformation in C3H 10T1/2 fibroblasts. Carcinogenesis 14, 1555-1559 (1993)   DOI   ScienceOn
16 Nomura, K., Yamaguchi, M. & Abiko, Y. Inhibition of interleukin-1beta production and gene expression in human gingival fibroblasts by low-energy laser irradiation. Lasers Med. Sci. 16, 218-223 (2001)   DOI   ScienceOn
17 Kroncke, K.D., Fehsel, K. & Kolb-Bachofen, V. Inducible nitric oxide synthase in human diseases. Clin Exp Immunol 113, 147-156 (1998)   DOI   PUBMED
18 Marletta, M.A., Hurshman, A.R. & Rusche, K.M. Catalysis by nitric oxide synthase. Curr. Opin. Chem. Biol. 2, 656-663 (1998)   DOI   ScienceOn