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http://dx.doi.org/10.5051/jpis.2012.42.4.136

Novel analysis model for implant osseointegration using ectopic bone formation via the recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system in rats: a proof-of-concept study  

Park, Jung-Chul (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
Lee, Jong-Bin (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
Daculsi, Guy (Research Center on Materials of Biological Interest, Dental faculty, Nantes University)
Oh, Sang-Yeop (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
Cho, Kyoo-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
Im, Gun-Il (Department of Orthopedics, Dongguk University Ilsan Hospital)
Kim, Byung-Soo (School of Chemical and Biological Engineering, Seoul National University)
Kim, Chang-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.42, no.4, 2012 , pp. 136-143 More about this Journal
Abstract
Purpose: The osseointegration around titanium mini-implants installed in macroporous biphasic calcium phosphate (MBCP) blocks was evaluated after incubation with recombinant human bone morphogenetic protein-2 (rhBMP-2) in an ectopic subcutaneous rat model. Methods: Mini-implants (${\varphi}1.8{\times}12$ mm) were installed in MBCP blocks (bMBCPs, $4{\times}5{\times}15$ mm) loaded with rhBMP-2 at 0.1 mg/mL, and then implanted for 8 weeks into subcutaneous pockets of male Sprague-Dawley rats (n=10). A histomorphometric analysis was performed, and the bone-to-implant contact (BIC) and bone density were evaluated. Results: Significant osteoinductive activity was induced in the rhBMP-2/bMBCP group. The percentage of BIC was $41.23{\pm}4.13%$ (mean${\pm}$standard deviation), while bone density was $33.47{\pm}5.73%$. In contrast, no bone formation was observed in the bMBCP only group. Conclusions: This model represents a more standardized tool for analyzing osseointegration and bone healing along the implant surface and in bMBCPs that excludes various healing factors derived from selected animals and defect models.
Keywords
Animals; Dental implants; Osseointegration;
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