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http://dx.doi.org/10.5051/jpis.2010.40.3.132

Bone regeneration effects of human allogenous bone substitutes: a preliminary study  

Lee, Deok-Won (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Koo, Ki-Tae (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Seol, Yang-Jo (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Lee, Yong-Moo (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Ku, Young (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Rhyu, In-Chul (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Chung, Chong-Pyoung (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Kim, Tae-Il (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.40, no.3, 2010 , pp. 132-138 More about this Journal
Abstract
Purpose: The purpose of this study was to compare the bone regeneration effects of cortical, cancellous, and cortico-cancellous human bone substitutes on calvarial defects of rabbits. Methods: Four 8-mm diameter calvarial defects were created in each of nine New Zealand white rabbits. Freeze-dried cortical bone, freeze-dried cortico-cancellous bone, and demineralized bone matrix with freeze-dried cancellous bone were inserted into the defects, while the non-grafted defect was regarded as the control. After 4, 8, and 12 weeks of healing, the experimental animals were euthanized for specimen preparation. Micro-computed tomography (micro-CT) was performed to calculate the percent bone volume. After histological evaluation, histomorphometric analysis was performed to quantify new bone formation. Results: In micro-CT evaluation, freeze-dried cortico-cancellous human bone showed the highest percent bone volume value among the experimental groups at week 4. At week 8 and week 12, freeze-dried cortical human bone showed the highest percent bone volume value among the experimental groups. In histologic evaluation, at week 4, freeze-dried cortico-cancellous human bone showed more prominent osteoid tissue than any other group. New bone formation was increased in all of the experimental groups at week 8 and 12. Histomorphometric data showed that freeze-dried cortico-cancellous human bone showed a significantly higher new bone formation percentile value than any other experimental group at week 4. At week 8, freeze-dried cortical human bone showed the highest value, of which a significant difference existed between freeze-dried cortical human bone and demineralized bone matrix with freeze-dried cancellous human bone. At week 12, there were no significant differences among the experimental groups. Conclusions: Freeze-dried cortico-cancellous human bone showed swift new bone formation at the 4-week healing phase, whereas there was less difference in new bone formation among the experimental groups in the following healing phases.
Keywords
Bone Substitutes; Osteogenesis; Transplantation; X-Ray Microtomography;
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