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The effects of novel biodegradable amorphous Calcium Phosphate on bone regeneration in rat calvarial defects  

Choi, Jung-Yoo (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Chae, Gyung-Joon (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Kim, Chang-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Lee, Yong-Keun (Department of Periodontology, Oral Science Research Center, College of Dentistry, Yonsei University)
Cho, Kyoo-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Chai, Joong-Kyu (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Kim, Chong-Kwan (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University)
Publication Information
Journal of Periodontal and Implant Science / v.37, no.4, 2007 , pp. 871-879 More about this Journal
Abstract
Purpose: The purpose of this study was to evaluate the bone regeneration of novel biodegradable amorphous calcium phosphate. Materials and Method: An 8-mm, calvarial, critical-size osteotomy defect was created in each of 20 male Sprague-Dawley rats(weight $250{\sim}300g$). The animals were divided into two groups of 10 animals each and allowed to heal for 2 weeks(10 rats). The first group was the control group and the other group was the experimental group which received the novel biodegradable calcium phosphate. Results: The healing of the calvarium in the control group was uneventful. The histologic results showed little bone formation in the control group. The experimental group which received the novel biodegradable calcium phosphate showed a normal wound healing. There were a lot of new bone formation around the biomaterial in 2 weeks. The bone formation increased in 8 weeks when compared to 2 weeks and there was a significant bone increase as well(P<0.01). The nobel biodegradable calcium phosphate showed statistical significance when compared to the control group (P<0.05). The novel biodegradable calcium phosphate in 8 weeks showed a significant increase in bone formation when compared to 2 weeks $(40.4{\pm}1.6)$(%). The biodegradable calcium phosphate which is made from mixing calcium phosphate glass(CPG), NaCO and NaOH solution, is biocompatible, osteoconductive and has a high potency of bone formation. Conclusion: We can conclude that the novel biodegradable calcium phosphate can be used as an efficient bone graft material for its biodegradability and osteoconductivity.
Keywords
novel biodegradable calcium phosphate; bone; bone regeneration;
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1 Stahl SS. Froum SJ: 'Histologic evaluation of human intraosseous healing response to the placement of tricalcium phosphate ceramic implant.' J. Periodontology., 57:211-217. 1986   DOI
2 Zamet JS, Dabar UR, Particulate bioglass as a grafting material in treatment of periodontal infrabony defects. J. Clin. Periodontol 24: 410-418, 1997   DOI   ScienceOn
3 Garrett S. Periodontal regeneration around natural teeth. Ann Periodontol 1;621-666, 1996   DOI   PUBMED   ScienceOn
4 W. E. Brown, L.C. Chow. Cements Research Progress; 352, 1986
5 Y. -K. Lee, J. Biomed. Mater. Res., Vol. 69A; 188, 2004   DOI
6 Y. -K. Lee. Key Eng. Materl, Vol. 254-156; 185, 2004
7 Hong SJ, Kim CS, Jung UW, et al. Fibrin- Fibronectin Sealing System in Combination with-Tricalcium Phosphate as a Carrier for Recombinant Human Bone Morphogenetic Protein-2: Effects on Bone Formation in Rat Calvarial Defects Biomaterials 2005 accepted for publication
8 Illi OE, Feldmann CP. Stimulation of fracture healing by local application of humoral factors integrated in biodegradable implants. Eur J pediatr Surg, 8: 251-255, 1998   DOI   ScienceOn
9 Soldheim E. Growth factors in bone. lntl Orthop; 22: 410-416, 1998   DOI
10 Gross JM, Lickfield GC, Latour RA. Calcium phosphate reinforced bioabsorbable polymer composites. Prec 23rd meeting of biomaterials; 159, 1997
11 Hyun SJ, Choi SH, Chai JK et al. The effect of recombinant human bone morphogenetic protein-2, 4 and 7 on bone formation in rat calvarial defects. J Periodontol 2005;76:1-8   DOI   ScienceOn
12 Linkhart TA, Mohan S. Baylink DJ. Growth factors for bone growth and repair: IGF, TGF-B and BMP. Bone;19: 1-12, 1996   DOI   ScienceOn
13 Carranza FA Jr, Kenny EB, Lekovic V, Taltmante E, Valencia J and Dimitrijevic B: 'Histologic study of the healing of human periodontal defect after placement of porous hydroxyapatite implants.' J. Periodontol. 58:682-688, 1987   DOI   PUBMED
14 Kawazoe Y, Shiba T. Induction of calcification in MC3 T3-E1 cells by inorganic polyphosphate. J. Dent Res., 83: 613-618, 2004   DOI   ScienceOn
15 Schmitt JM, Hwang K, Winn SR, Hollinger JO. Bone morphogenetic proteins: an update on basic biology and clinical relevance. J Orthop Res; 17: 269-278, 1999   DOI   ScienceOn
16 Yukna RA, Clinical evaluation of HTR polymer bone replacement grafts in human mandibular class II molar furcation, J. Periodontol 65: 342-349, 1994   DOI   PUBMED   ScienceOn
17 Bostrom MPG, Asnis P. Transforming growth factor beta in fracture repair. Clin Orthop Rel Res; 355(Suppl.): 124-131, 1998
18 B.H. Lee, M.C. Kim et. al: Biodegradable Bone Cement Using Calcium Phosphate Glass, Key Enineering Materials, 2006; Vols. 309-311, 861-864   DOI
19 Hiatt MA, Schallhorn RG, Aaronian AJ: 'The induction of new bone and cementum formation : IV. Microscopic examination of the periodontium following human bone and marrow allograft, autograft and non-graft regenerative procedures.' J. Periodontol., 49:495-512, 1978   DOI   PUBMED
20 Leyhausen G, Lorenz B. Inorganic polyphosphate in human osteoblast-like cells, J. Bone Miner Res, 13: 803-812, 1998   DOI   ScienceOn
21 이진용, 류동목, 신제원, 무기인산염의 골재생 촉진효과에 관한 연구. 대한구강해부학회지1999;23:219-228
22 Yukna RA, Clinical evaluation of coralline carbonate as a bone replacement graft material in human periodontal osseous defects. J Periodontol 65; 177-185, 1994   DOI   PUBMED   ScienceOn
23 Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunion. Clin Orthop 1986; 205: 299-308
24 Brunsvold MA, Mellonig JT. Bone grafts and periodontal regeneration, Periodontal 2000. 1:80-91, 1983
25 Takagi K, Urist MR. The reaction of the dura to bone morphogenetic protein(BMP) in repair of skull defect. Ann Surg 1982; 196:100-109   DOI   PUBMED   ScienceOn
26 Latour RA. Fiber-reinforced composites biomaterials for orthopedic implant applications. Encyclopedia of Biomaterials and Bioengineering, 359-382, 1995
27 Han DK, Kim CS, Cho KS. Effect of a Fibrin-fibronectin sealing system as a carrier for recombinant sealing system as a carrier for recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects. J Periodontol 2005 accepted for publication
28 Andrino KP, Daniels AJ. Biocompatibility and mechanical properties of an totally absorbable composite material for orthopedic fixation devices. J Appl Biomat 3; 197-206, 1992   DOI   ScienceOn
29 Pang EK, Im SU, Kim CS, et al. Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in rat calvarial defects. J Periodontol 2004;75:1364-1370   DOI   ScienceOn