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Comparative study on the Effects of Platelet-Rich Plasma and Enamel Matrix Protein on Supracrestal bone Regeneration of Dental Implant  

Eun, Hee-Jong (Department of Periodontology, College of Dentistry, Dan-Kook University)
Lim, Sung-Bin (Department of Periodontology, College of Dentistry, Dan-Kook University)
Chung, Chin-Hyung (Department of Periodontology, College of Dentistry, Dan-Kook University)
Hong, Ki-Seok (Department of Periodontology, College of Dentistry, Dan-Kook University)
Lee, Chong-Heon (Department of Pathology, College of Dentistry, Dan-Kook University)
Publication Information
Journal of Periodontal and Implant Science / v.35, no.1, 2005 , pp. 235-250 More about this Journal
Abstract
The current interest in periodontal tissue regeneration has lead to research in bone graft, root surface treatments, guided-tissue regeneration, administration of growth factors, and the use of enamel matrix protein as possible means of regenerating lost periodontal tissue. Several studies have shown that a strong correlation between platelet-rich plasma and the stimulation of remodeling and remineralization of grafted bone exits, resulting in a possible increase of 15-30% in the density of bone trabeculae. The purpose of this study was to study the histopathological results and differences between the use of platelet-rich plasma and the use of enamel matrix $protein(Emdogain^?)$ about bone regeneration at the implant. Implant fixtures were inserted and graft materials placed into the left femur in the experimental group, while the only implant fixtures placed in the control group. In the first experimental group, platelet-rich plasma and xenograft were placed at the supracrestally placed implant site, and in the second experimental group, $Emdogain^{(R)}$ and xenograft placed at the supracrestally placed fixture site. The degree of bone regeneration adjacent to the implant fixture was observed and compared histopathologically at 2, 4, and 8 weeks after implant fixture insertion. The results of the experiment are as follows: 1. The rate of osseointegration to the fixture threads was found to be greater in the experimental group compared to in the control group. 2. The histopathological findings showed that the bone regeneration, the partial osseointegration existed at 4 weeks, and that osseointegration and bone density increaced in the experimental groups at 8 weeks. 3. The results showed that new bone formation and bone remodeling increased in the area near to the fixture in the first and second experimental groups at 8 weeks than at 4 weeks. The results showed that in the area distant from the fixture, new bone formation did not increase and bone remodeling decreased in the first experimental group at 4, 8 weeks, and that new bone formation increased in the second experimental group. 4. The histopathological findings showed that AZ deposition in the first experimental group was remarkable at 2, 8 weeks, and in the second experimental group at 2, 4, 8 weeks in the area distant from the fixture threads.
Keywords
Platelet-Rich Plasma; Enamel Matrix Protein; bone regeneration;
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1 Wikesjo UME, Baker PJ, Christersson LA, Genco RJ, Lyall RM, Hic S, Diflorio RM, and Terranova VP : A biochemical approach to periodontal regeneration: Tetracycline treatment conditions dentin surfaces, J Periodontal Res 1986;21;322-329   DOI   PUBMED
2 Garret S, Criger M, and Egelberg J : Effect of citric acid on diseased root surface. J Periodontal Res 1978;13;155-163   DOI   PUBMED
3 Ririe, CM, Crigger M, and Selving KA : Healing of periodontal connective tissues following surgical wounding and application of citric acid in dogs. J Periodontal Res 1980;15;314-327   DOI   PUBMED
4 Andreasen JO : Periodontal healing after replantation and autotransplantation of permanent incisors. Int J Oral Surg 1981;60;54-61
5 Gunnar H : Treatment of human periodontal infrabony defects with Emdogain, Case report : Clinical and radiographic examination after one year of treatment. Int J Periodontics Restorative Dent 2000;1;19-31
6 백승호, 손호현, 임성삼 : 성견에서 Super EVA, Ketac silver, MTA와 Emodgain을 이용한 치근분지부 천공치유에 관한 연구. 대한치과보존학회지 2000;25;2;159-165
7 Mellonig JI' : Enamel matrix derivative for periodontal reconstructive surgery: Technique, clinical and histologic case report. Int J Periodontics Restorative Dent 1999;19;8-19   PUBMED
8 Lynch SE, Williams RC, Polson AM, Howell TH, Reddy Ms, Zappa UE, and Antoniades HN : A combination of platelet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 1989;16;545-548   DOI   PUBMED
9 Postlethwaite AE, Keski-oja J, Moses ML, and Kang AH : Stimulation of the chemotatic migration of human fibroblasts by transforming frowth factor beta. J Exp Med 1987;165;251-256   DOI   ScienceOn
10 Canalis E : Effect of insulin-like growth factor I on DNA and protein synthesis in cultured rat calvaria. J Clin Invest 1980;66;709-719   DOI   ScienceOn
11 Wilson TG Jr, Schenk R, Buser D, and Cochran D : Implants placed in immediate extraction sites: a report of histologic and histometric analyses of human biopsies. Int J Oral Maxillofac Implants. 1998;13(3);333-341
12 Canalis E, Carthy TL, and Centrella M : The role of growth factors in skeletal remodelling. Endocrimol Meta Clin North Am 1989;18:903-912
13 Kassolis JD, Rosen PS, and Reynolds MA : Alveolar ridge and sinus augmentation utilizing platelet rich plasma in combination with freeze dried bone allograft : case series. J Periodontol 2000;71;1654-1661   DOI   ScienceOn
14 Cho MI, Garant PR : Radioautographic study of [3H]mannose utilization during cementoblast differentiation, formation of acellular cementum, and development of periodontal ligament principal fibers. Anat Rec. 1989;223(2);209-222.   DOI   ScienceOn
15 Franke SV, Johansson CB : Enamel matrix derivative and titanium implants. J Clin Periodontol. 2003;30(4) ;359-363   DOI   ScienceOn
16 Raymond A, Yukna : Histologic evaluation of periodontal healing in humans following regenerative therapy with enamel matrix derivative. J Periodontol 2000;71;752-759   DOI   ScienceOn
17 Karring J, Nymann S, Lindhe J, and Sirirat M : Healing following implantation of periodontitis-affected roots into bone tissue. J Clin Periodontol 1980;7;96-105   DOI   PUBMED
18 고호경, 채중규 : Oxidized cellulose membrane을 이용한 replamineform hydoxyapatite 및 calcium catbonete와 혼합사용시 성견 치주조직의 치유에 미치는 영향. 대한치주과학회지 1992;22;201
19 Anton S : The effect of postsurgical antibiotics on the healing of intrabony defect following treatment with enamel matrix protein. J Periodontol 2001;72;190-195   DOI   ScienceOn
20 최형호, 김정근, 임성빈, 정진형 : PDGF가 조골세포의 활성에 미치는 영향. 대한치주과학회지 1999;29;4;785-802
21 Roberts AB, Spron MB : Physioligical actions and clinical application of transforming growth factor- ${\beta}$. Growth factor 1993;8;1-9   DOI   ScienceOn
22 Sigurdsson TJ, Hardwick R, Bogle GC, and Wikesjo UME : Periodontal repair in dogs : Space provision by reinforced e-PTFE membranes enhance bone and cementum regeneration in large supra-alveolar defects. J Periodontol 1994;65;350
23 Wergesal JE, Mohan S, Lundy M, and Baylink DJ : Skeletal growth factor and other factors known to be present in bone matrix stimulate proliferation and protein synthesis in human bone cells. J Bone Miner Res 1990;5;179-186   DOI   PUBMED   ScienceOn
24 Hammarstrom L, Heijl L, and Genstrelius S : Peridontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. J Clin Periodontol 1997;24;669-677   DOI   PUBMED   ScienceOn
25 Marx RE, Carlson ER, Eichstaedt RH, Schimmele SR, Strauss JE, and Georgeff KR : Platelet-rich plasma : Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol 1998;85;638-646   DOI   ScienceOn
26 Kassolis JD, Rosen PS, and Reynolds MA : Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freezedried bone allograft; case series. J Periodontol 2000;71;1654-1661   DOI   ScienceOn
27 Rummelhart JM, Mellonig JT, Gray JL, and Towle HJ : A comparision of freeze-dried bone allograft and demineralized freeze-dried bone allograft in human periodontal osseous defects. J Periodontol 1989;60;655-663   DOI   PUBMED
28 Deporter DA, Watson PA, and Pillar RM : A histological assessment of the initial healing response adjacent to porous-surface, titanium alloy dental implant system in dogs. J Dent Res. 1986;65; 1064-1070   DOI   PUBMED   ScienceOn
29 Miyazono K, Ten-Dijke P, Ichiyo H, and Heldin CH : Receptors for transforming growth factor- ${\beta}$ Immunol 1994;15:181-220
30 Rutherford RB, Trilsmith MD, Ryan HE, and Charette MF : Synergistic effects of dexamethasone on platelet -derived growth factors mitogenesis in vitro. Arch Oral Biol 1992;37;139-145   DOI   ScienceOn
31 Lozada JL, Caplanis N, Proussaefs P, Willardsen J, and Kammeyer G : Platelet-rich plasma application in sinus graft surgery; Part I-Background and processing techniques. J Oral Implantol. 2001 ;27;38-42   DOI   ScienceOn
32 Formicola AJ, Krampf JI, and Witte ET : Cementogenesis in developing rat molars. J Periodontol, 1971;42(12);766-773   DOI   PUBMED
33 Slavkin HC : Towards a cellular and molecular understanding of periodontics. Cementogenesis revisited. J Periodontol. 1976;47(5);249-255   DOI   PUBMED
34 Mellonig JT : Autogenous and allogenic bone grafts in periodontal therapy. Crit Rev Oral Biol 1992;3;333-352
35 Terranova VP, Odziemiec C, Tweden KS, and Spadone DP : Repopulation of dentin surfaces by periodontal ligament cells and endothelial cells. J Periodontol 1989;60;293-301   DOI   PUBMED
36 Turner DW, Melloonig JT : Antigenicity of freeze-dried bone allograft in periodontal osseous defects J. Periodontal Res 1981;16(1);89-99   DOI   PUBMED
37 Hammarstrom L : Enamel matrix cementum development and regeneration. J Clin Periodontol 1997;24;658-668   DOI   ScienceOn
38 Antonaides HN, Williams LT : Human plateletderived growth factor; structure and functions. Federation Proc 1983;42;2630-2634
39 Rosen PS, Reynolds MA : A retrospective case series comparing the use of demineralized freeze-dried bone allograft and freeze-dried bone allograft combined with enamel matrix derivative for the treatment of advanced osseous lesions. J Periodontol. 2002;73(8);942-949   DOI   ScienceOn
40 Lu HJ : Topographical characteristics of root trunk length related to guided tissue regeneration. J Periodontol 1992;63;215-219   DOI   PUBMED
41 Adell R, Lekholm U, Rocker B, and Branemark PI : A IS-year study of Osseointergrated implants in the treatment of the edentulous jaw. Int J Oral Surg, 1981;10;387-416   DOI   PUBMED
42 Marx RE, Saunders TR : Reconstuction and rehabilitation of cancer patients. Reconstuctive Prosthetic Oral and Maxillofacial surgery Philadelphia; Saunders 1986;347-427
43 Gottlow J, Nyman S : New attachment formation as the result of controlled tissue regeneration. J Clin Periodontol 1984;11;494-503   DOI   PUBMED
44 Stuart JF : A comparative study utilizing open flap debridement with and without enamel matrix derivative in the treatment of periodontal intrabony defect. J Periodontol 2001;72;25-34   DOI   ScienceOn
45 Babbush CA, Kent JN, and Misiekm DJ : Titanium plasma sprayed(TPS) screw implants for the reconstruction of the edentulous mandible. J Oral Maxillofac Surg, 1986;44;274-282   DOI   ScienceOn
46 Caton J, Greenstein G, and Zappa U : Synthetic bioabsorbable barrier for regeneration in human periodontal defects. J Periodontol 1994;65;1037-1045   DOI   PUBMED   ScienceOn
47 Stahh SS : Repair potential of the soft tissue root interface. J Periodontol 1977;48;545-552   DOI   PUBMED
48 Brooks DB, Heiple KG : Immunological factors in homogeneous bone transplantation. IV. The effect of various methods of preparation and irradiation on antigenicity. J. Bone Joint Surg 1963;45;16-17
49 Robert E, Marx, and Eric R : Platelet rich plasma growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Indod 1998;85;638-646   DOI   ScienceOn
50 Meffert RM, Thomas JR : Hydroxyapatite as an alloplastic grafts in the treatment of human periodontal osseous defects. J Periodontol 1985;56;63-73   PUBMED
51 박상일, 정진형, 임성빈, 김정근 : 혈소판 농축혈장이 조골세포의 초기부착과 증식 및 활성에 미치는 생물학적 영향. 대한치주과학회지 2001;31;3;513-529
52 Terranova VP, Franzetti LC, Hick S, and Wikesj VME : Biochemically mediated periodontal regeneration. J Periodontal Res 1987;22;248-251   DOI   PUBMED
53 Venezia E, Goldstein M, and Schwartz Z : The use of enamel matrix derivative in periodontal therapy. Refuat Hapeh Vehashinayim. 2002;19(3):19-34   PUBMED
54 Nyman S, Karing J, Lindhe J, and Platen S : Healing following implantation of periodontitis-affected roots into gigival connective tissue J Clin Periodontol 1980;7;394-401   DOI   PUBMED
55 Linch SE, Castillia GR, Williams RC, Kiritry CP, Howell TB, and Reddy MS : The effects of shortterm application of a combination of plateletderieved and insulin-like growth factors on periodontal wound healing. J Periodontol 1991;62;458-467   DOI   PUBMED
56 Izeng DY, Deuel TF, Hwang JS, and Baehner RL : Platelet-derived growth factor promotes human peripheral monocyte activation. Blood 1985;66;179-183   PUBMED
57 Keski-oja J, Leof EB, Lyons RM, Coffey RJ Jr, and Moses ML : Transforming growth factor and control of neoplastic cell growth. J cell Biochem 1987;33;95-107   DOI   PUBMED
58 Anitua E : Plasma rich in growth factors Prelimenary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14;529-535   PUBMED
59 Giannobile WV, Finkelman RD, and Lynch SE : Comparison of canine and non-human primate animals models for periodontal regenerative therapy : Results following a single administration of PDGF/IGF-I. J periodontol 1994;65;1158-1168   DOI   PUBMED   ScienceOn
60 Anton S, Giovanni C, Peter W, and Nicolaos D : Enamel matrix derivative(Emdogain) for human periodontal reconstructive surgery: clinical and histologic evaluation. Int J Periodontics Restorative Dent 2001;2;49-55
61 Sporn MB, Roberts AB, Wakefield LM, and de crombrugghe B. : Some recent advances in the chemistry and biology of transforming growth factor beta. J Cell Biol 1987;105;1039-1045   DOI   ScienceOn
62 Bauer EA, Cooper TW, Hwang JS, Altman J, and Deuel TF : Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor. Proc Natl Acad Sci USA 1985;82;4132-4136   DOI
63 Kohal RJ, Mellas P, Hurzeler MB, Trejo PM, Morrison E, and Caffesse RG : The effects of guided bone regeneration and grafting on implants placed into immediate extraction sockets. An experimental study in dogs. J Periodontol. 1998;69(8) ;927-937   DOI   PUBMED   ScienceOn
64 Caffesse RG, Smith BA : New attachment achieved by guided tissue regeneration in the beagle dogs. J Periodontol 1988;59;589-594   DOI   PUBMED
65 Lynch SE, Colvin RV, and Antoriades HN : Growth factors in wound healing : Single and synergistic effects on the partial thickness porcine skin wounds. J Cin Invest 1989;84;640-646   DOI
66 Giulio R, Giano R, and Maurizio S : Enamel matrix derivative for periodontal reconstructive surgery. Int J Periodontics Restorative Dent 2000;1;59-67
67 Mathys EC : Immediate implants: indications and clinical decision making : J Colo Dent Assoc. 1998 Autumn;77(4);20-25   PUBMED
68 Antoniades HN, Scher CD, and Stiles CD : Purification of human platelet-derived growth factor. Cell Biol 1979;76:1809-1813
69 Bosshardt DD, Schroeder HE : Initiation of acellular extrinsic fiber cementum on human teeth. A light- and electron-microscopic study. Cell Tissue Res. 1991;263(2);311-324   DOI   ScienceOn
70 Farjava H, Salonen J, Hakkinen L, and Narhi T : Effect of citric acid treatment on the migration of epithelium on root surface in vitro. J Periodontol 1988;59;95-101   DOI   PUBMED
71 Sculean A, Donos N, Blaes A, Reich E, and Brecx M : Enamel matrix proteins(Erndogain) and guided tissue regeneration in the treatment of intrabony periodontal defects: A split-mouth clinical study. J Dent Res 1998;77;924-929
72 Terranova VP, Hick S, Franzetti L, Lyall RM, and Wikesso VME : A biochemical approach to periodontaol regeneration, AFS CM, Assay for specific cell migration. J Periodontol 1987;58;247-259   DOI   PUBMED
73 Karring J, Nymann S, Lindhe J, and Sirirat M : Potentials for root resorption during periodontal wound healing. J Clin Periodontol 1984;11;41-52   DOI   PUBMED
74 Sculean A, Chiantella GC, Windisch P, Gera I, and Reich E : Clinical evaluation of an enamel matrix protein derivative (Erndogain) combined with a bovine-derived xenograft (Bio-Oss) for the treatment of intrabony periodontal defects in humans. Int J Periodontics Restorative Dent. 2002;22(3);259-267
75 Machtei EE, Cho MI, Dunford R, Norderyd J, Zambon JJ, and Genco RJ: Clinical, microbiological, and histological factors which influence the success of regenerative periodontal therapy. J Periodontol 1994;65;154-161   DOI   PUBMED   ScienceOn
76 Ignortz R, Massgue I : Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into extracellular matrix. J Biol Chem 1986;261;4337-4345   PUBMED
77 Heijl L, Heden G, svardstrom G, and Ostgren A: Enamel matrix derivative(Emdogain) in the treatment of infrabony periodontal defects. J Clin Periodontol 1997;24;705-714   DOI   PUBMED   ScienceOn
78 Howell TH, Fiorellini JP, and Paquette DW : A phase I/II clinical trial to evaluation a combination of recombinant human insulin-like growth factor-I in patients with periodontal disease. J Periodontol 1997;68;1186-1193   DOI   PUBMED   ScienceOn
79 Louise F : Histologic case reports of coralline hydroxyapatite grafts placed in human intraosseous lesions : Results 6 to 36 months postimplantation. Int J Periodont Rest Dent 1992;12(6);475-483
80 Hawiger J : Platelet secretory pathway : An overview, Method,Enzyme. 1989;169;191-195   DOI
81 Albrektsson T, Dahl E, and Enbom L : Osseointegrated oral implants. A Swedish multicenter study of 8139 consecutively inserted nobelpharma implants. J Periodontol. 1988;59;287-296   DOI   PUBMED
82 Bowen-Dope DF, Vogel A, and Ross R : Production of platelet derived growth factor like molecules and reduced expression of platelet derived growth factor receptors accompany transformation by a wide spectrum of agents. Proc Natl Acad Sci USA 1984;81;2396-2400   DOI
83 Polson AH : The root surface and regeneration : Present therapeutic limitations and future biologic potentials. J Clin Periodontol 1986;13;955-996
84 Casati MZ, Sallum EA, Nociti FH Jr, Caffesse RG, and Sallum AW : Enamel matrix derivative and bone healing after guided bone regeneration in dehiscence-type defects around implants. A histomorphometric study in dogs. J Periodontol. 2002;73(7);789-796   DOI   ScienceOn