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Effect of Enamel Matrix Drivatives application on the expression of PDLs17, PDLs22 of cultured human periodontal ligament cells in vitro  

Han, Geun-A (Dept, of periodontology, college of dentistry, Chosun University)
Jang, Hyun-seon (Dept, of periodontology, college of dentistry, Chosun University)
Kok, Jung-Ki (Dept, of biochemistry, college of dentistry, Chosun University)
Park, Ju-Chol (Dept, of oral histology, college of dentistry, Chosun University)
Kim, Heoung-Jung (Dept, of anatomy, college of dentistry, Chosun University)
Kim, Jung-Gwan (Dept, of periodontology, college of dentistry, Yunsei University)
Kim, Byung-Ock (Dept, of periodontology, college of dentistry, Chosun University)
Publication Information
Journal of Periodontal and Implant Science / v.34, no.2, 2004 , pp. 333-344 More about this Journal
Abstract
The enamel matrix derivative (EMD) has been recently used in the periodontal regenerative techniques. The present study was established to investigate the influence of EMD on human periodontal ligament cells using expression of mRNA of periodontal ligament specific gene (PDLs)17, PDLs22, type I collagen when EMD applied to periodontal ligament cells. Periodontal ligament cells were obtained from a healthy periodontium and cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum and ${\beta}-glycerophosphate$ with ascorbic acid. Test groups were two; One adds EMD in culture media and another added EMD and Dexamethasone (DEX) in culture media. Positive control group added DEX in culture media, and negative control group adds niether of EMD nor DEX. $Emdogain^{(R)}$ (Biora, Sweden, 30 mg/ml) was diluted by 75 ${\mu}g/ml$ concentration to culture media. For reverse transcription-polymerase chain reaction (RT-PCR), total RNA isolated on days 0, 7, 14 and 21. mRNA of PDLs17 was expressed on days 14 and 21 in EMD or DEX group, and expressed on days 7, 14 and 21 in EMD plus DEX group, the other side, expressed on days 21 in negative control group. mRNA of PDLs22 expressed on days 7, 14 and 21 in EMD group, and expressed on days 14 and 21 in DEX group, and expressed on days 7, 14 and 21 in EMD plus DEX group. Negative control group expressed on days 14 and 21. Type I collagen was expressed on all days and all groups. These results indicate that EMD promotes differentiation of periodontal ligament cells, and this is considered to offer basis that can apply EMD to periodontal tissue regeneration technique.
Keywords
Enamel Matrix Drivate; PDLs17; PDLs 22; periodontal ligament;
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  • Reference
1 McCulloch, C.A.G.. Basic Considerations in periodontal wound healing to achive regeneration. Periodontology 2000., 1:16-25, 1993
2 Howes, R. Bowness, J.M. Grotendorst, G.M et at .. Platelet-derived growth factor enhances demineralized bone matrix-induced cartilage and bone formation. Calcif Tissue Int., 42: 34-38, 1998
3 Nyman, S. Lindhe, J. Karring, T et al.. New attachment following surgical treatment of human periodontal disease. Journal of Clinical periodontology., 9: 290-296, 1982
4 Torneck, C.D.. Dentin-Pulp complex. In : Oral histology: Development, structure and function. ed Ten Cate, A.R. The mosby company, St Louis., 1980, pp140-181
5 Terranova, V.P. Wikesjo, U.M.E.. Extracellular matrices and polypeptide growth factors as mediators of functions of cells of the periodontium. Journal of Periodontology ., 58: 371-380
6 Raymond, A. Yukna.. Histologic evaluation of periodontal healing in humans following regenerative therapy with enamel matrix derivative. Journal of Periodontololgy., 72: 752-759, 2000
7 Pontoriero, R et al., The use of barrier membranes and enamel matrix proteins in the treatment of angular bone defects. Journal of Clinical Periodontology., 26:833-840, 1999
8 Amar, S.. Implications of cellular and molecular biology advances in periodontal regeneration. Anatomical Record., 245:361-373, 1996
9 Iglhaut, J. Aukhil, I. Simpson, D.M. et al.. Progenitor cell kinetics during Guided tissue regeneration in experimental periodontal wounds. Journal of Periodontal Research., 23: 107-117,1988
10 Haae, H.R. Bartold, P.M. Enamel matrix derivative induces matrix synthesis by cultured human periodontal fibroblast cells. Journal of Periodontology., 72: 341-348, 2001
11 Tenenbaum, H.C. Heersche, J.N.M.. Dexamethasone stimulates osteogenesis in click periosteum in vitro. Endocrinology., 117: 2211-2217, 1985
12 Nohuteu, R.M. Somerman, M.J. McCauley, L.K.. Dexamethasone enhances the effects of parathyroid hormone(PTH) on human periodontal ligament cells in vitro. Calcif Tissue Int. , 56: 571-577, 1995
13 Bosshardt, D.D. Nand, A.. Immunolocalization of epithelial and mesenchymal matrix constituents in association with inner and outer epithelial cells. Journal of Histochem Cytochem. , 46: 135-142, 1998
14 Reddi, A.H. Cunningham, N.S.. Initiation and promotion of bone differentiation by bone morphogenic proteins. Journal of Bone Miner Res ,, 8(suppl 2): S499-502, 1993
15 Gastrelius, S. Andersson, C. Lidstrom, D. et al.. In vitro studies on periodontal ligament cells and enamel matrix derivative. Journal of Clinical Periodontology., 24: 685-692, 1997
16 Mukai, M. Yoshimine, Y. Akamine, A. et al.. Bone-like nodules formed in vitro by rat periodontal ligament cells. Cell Tissue Res., 271: 453-460, 1993
17 Karring, T. Nyman, S. Gottrow, J et al .. Development of the biological concept of guided tissue regeneration-animal and human stud-ies, Periodontology 2000., 1: 26-35, 1993
18 Egellberg, J.. Regeneration and repair of periodontal tissue. Journal of Periodontal Research., 22: 233-242, 1987
19 Araujo, M. Lindhe, J.. GTR treatment of degree III furcation defects following application of enamel matrix proteins An experimental study in dogs. Journal of Clinical Periodontology ., 25: 524-530, 1998
20 Mellonig, J.T. Enamel matrix derivative for periodontal reconstructive surgery: Technique, clinical and histologiccase report. Int J Periodontic Restorative Dent ., 19:8-19,1990
21 Caton, J. Nyman, S. Zander, H.. Histometric evaluation of periodontal surgery. II. Connective tissue attachment levels after four regenerative procedures. Journal of Clinical Periodontology., 7: 224-231, 1980
22 Oates, T.W. Rouse, C.A. Cochran, D.L.. Mitogenic effects of growth factors on human periodontal ligament cellsin vitro. Journal of Periodontology., 64: 142-148, 1993.   DOI   ScienceOn
23 Remarkrishnan, P.R. Lin, W.L. Sadek,J. et al.. Synthesis of noncollagenous extracellular matrix proteins during development of mineralized nodules by rat periodontal ligament cells in vitro. Calcif Tissue Int., 57: 52-59, 1995
24 Cho, M.I. Garant, P.R.. Development and general structure of the periodontium. Periodontology 2000., 24: 9-27, 2000
25 Giulio, R. Giano, R. Maurizio, S.. Enamel rnatrix derivative for periodontal reconstructive surgery, Int J Periodontics Restorative Dent., 1: 59-67, 2000
26 Sculean, A. Donos, N. Blaes, A. et al.. Enamel matrix proteins(Emdogain) and Guided tissue regeneration in the treatment of intrabony periodontal defects: A split-mouth clinical study. Journal of Dent Res., 77: 924-929, 1998
27 Marx, R.E. Carlson, E.R. Eichstedt, R.M. et al.. Platelet-rich plasm :Growth factor enhancement for bone grafts. Oral asurg Oral Med Oral Pathol., 85: 638-546, 1998
28 Brandsten, C. Lundmark, C. Christersson, C. et al., Expression of collagen $\alpha$ 1(I)mRNA variants during tooth and bone formation in the rat. Journal of Dental Research., 78(1): 11-19,1999
29 Lyngstadaas, S.p. Lundberg, E. Ekdahl, H. et al., Autocrine growth factors in human periodontal ligament cells cultured on enamel matrix derivative. Journal of Clinical Periodontology., 28: 181-188, 2001
30 Nilda, A. John, J.S. Jennifer, M Et al., Human periodontal cells initiate mineral-like nodules in vitro. Journal of Periodontology., 62: 499-503, 1991
31 Melcher, A.H.. On the repair potential of periodontal tissue. Journal of periodontology., 47: 256-260, 1976
32 Araujo, M. Berglundh, T. Lindhe, J.. The Periodontal tissue in healed degree ill furcation defects. An experimental study in dogs. Journal of Clinical Periodontology., 23: 532-541, 1996
33 Anitua, E.. Plasma rich in growth factors:Preliminary results of use in the preparationof future sites for implants. Int J Oral Maxillofac Implants., 14: 529-535, 1994
34 Hammarstrom, L. Heijl, L. Gestrelius, S.. Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. Journal of Clinical Periodontology., 24: 669-677,1997
35 Park, J.C. Kim, H.J. Jang, H.S et al.. Isolation and characterization of cultured human periodontal ligament fibroblast-specific cDNAs. Biochem Biophys Res Commnun., 282(5): 1145-1153,2001
36 Stuart, J.F.. A comparative study utilizing open flap debridement with and without enamel matrix derivative in the treatment of periodontal intrabony defects. Journal of Periodontolology ., 72: 25-34, 2001
37 Hammarstrom, L.. Enamel matrix, cementum development and regeneration. Journal of Clinical Periodontology., 24: 658-668, 1997
38 Nyman, S. Gottlow, J. Karring, T et al., The regenerative potential of periodontal ligament. An experimental study in the monkey. Journal of Clinical Periodontology., 7:257-265, 1982.
39 Caffese, R.G. Quinones, C.R .. Polypeptide growth factors and attachment proteins in periodontal wound healing and regeneration. Periodontology 2000.,1: 69-79,1993
40 Jiang, D. DZiak, R. Lynch, S. E. et al., Modification of Bio-Oss with growth factors: Effects on osteobalstic cell proliferation. Journal of Periodontol., 69: 638-646, 1998
41 Anton, S.. The effect of postsurgical antibiotics on the healing of intrabony defect following treatment with enamel matrix protein. Journal of periodontololgy, , 72: 190-195,2001
42 Slavkin, H.C Bringas, P. Bessen, C. et al.. Hertwig's epithelial root sheath differentiation and initial cementum and bone formation during long term organ culture of mouse mandibular first molars using serumless, chemically-defined medium. Journal of Periodontal research ., 23:28-40, 1988
43 Dennison, D.K. Valline, D.R. Pinero, G.J et al.. Differential effect of TGF-$\beta$1 and PDGF on proliferation of periodontal ligament cells and gingival fibroblasts. Journal of Periodontolgy., 65: 641-648,1994
44 Monique T, V.D.P. Theo, V.D.B. Vincent, E. et al.. Enamel matrix derived protein stimulates attachment of periodontal ligament fibroblasts and enhances alkaline phosphatase activity and transforming growth factor $\beta$ release of periodontal ligament and gingival fibroblasts. Journal Periodontolgy., 71: 31-43, 2000
45 Serna, S.H. Janice, E,B. Martha,J.S. The effect of enamel matrix protein derivative on follicle cells in vitro. Journal of Periodontology., 72: 679-687, 2001
46 Arceo, N. Sauk, JJ. Moehring, J. et al., Human periodontal cells initiate mineral-like nodules in vitro. Journal of Periodontoiogy ., 62: 499-503, 1991
47 gunnar, H.. Treatment of human periodontal infrabony defects with Emdogain. Case report: Clinical and radiographic examination after one year of treatment. Int J Periodontics Restorative Dent., 1: 19-31,2000
48 Canalis, E. McCarthy, T. Centrella, M.. Effects of basic fibroblast growth factor on bone formation in vitro. Journal of Clinical Invest., 81: 1572-1577,1988
49 Hirooka, H.. The biological concept for the use of EMDOGAIN? True periodotnal regeneration. Quintessence International., 29: 624-630, 1998
50 Rahime, M.N. Laurie, K.M. Amy J.K. et al.. Expression of extracellular matrix proteins in human periodontal ligament cells during mineralization in vitro. Journal of Periodontology., 68: 320-327, 1997