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Effects of Cervi Parvum Cornu on Cell Cycle Regulation in Human Fetal Osteoblasts  

Yang, Dae-Seung (Department of Periodontology, School of Dentistry)
Kim, Hyun-A (Department of Periodontology, School of Dentistry)
Hyun, Ha-Na (Department of Periodontology, School of Dentistry)
You, Hyung-Keun (Department of Periodontology, School of Dentistry)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Shin, Hyung-Shik (Department of Periodontology, School of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.32, no.4, 2002 , pp. 811-825 More about this Journal
Abstract
Recently, many natural medicines, whose advantages are less side effects and possibility of long-term use, have been studied for their capacity, their anti-bacterial and anti-inflammatory effects and regenerative potential of periodontal tissues. Cervi Parvum Comu(CPC) have been traditionally study as an hale, growth. hematogenous, anti-aging, hack pain in Eastern medicine. The purpose of present study was to investigate the effects of CPC extract on cell cycle progression and its molecular mechanism in human fetal osteoblasts. CPC extracts (10 ${\mu}g/ml$) increased cell proliferation in the human fetal osteoblasts compared to non-supplemented control. There was no significant change in the G1 and S phase, hut a increase in the G2/M phase in 10 ${\mu}g/ml$ and 100 ${\mu}g/ml$ of CPC extracts group as compared to non-supplemented control. The protein expression of cyclin E, cdk 2, cycln D, cdk 4, and cdk 6 was higher than that of control group. The level of p21 was lower than that of control. But that of pRb and pl6 was not distinguished from control. These results indicate that the increase of cell proliferation by CPC extracts may be due t o the increased expression of cyclin E, cdk 2, cyclin D, cdk 4 and cdk 6, and the decreased expression of p21 in human fetal osteoblasts.
Keywords
Cervi Parvum Comu(CPC); Human fatal osteoblsts; cell cycle;
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