Browse > Article

Study on development of DNA probe for identification of Prevotella intermedia G8-9-3  

Bak, Jong-Sung (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University)
Kim, Se-Hoon (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University)
kim, Dong-Ki (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University)
Seong, Jin-Hyo (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University)
Kim, Byung-Ock (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University)
Kim, Jung-Ki (Dept. of Oral Biochemistry, College of Dentistry, Chosun University)
Publication Information
Journal of Periodontal and Implant Science / v.32, no.2, 2002 , pp. 281-290 More about this Journal
Abstract
The purpose of this study is to develop species-specific DNA probe for detection and identification of Prevotella intermedia (P. intermedia) G8-9K-3. This study procedure includes (1) whole-genomic DNA extraction of P. intermedia G8-9K-3 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot hybridization, (4) confirmation of strain-specific DNA probe by Southern blot hybridization, (5) determination of nucleotide sequences of strain-specific DNA probe. Twenty-eight recombinant plasmids containing Hind III-digested DNA fragments of P. intermedia G8-9K-3 were obtained. Reverse Dot Hybridization and Southern blot analysis data showed that one of them, Pig3, could be P. intermedia G8-9K-3-specific DNA probe. This datum indicates that this Pig3 DNA probe could be useful in detection and identification of the P. intermedia G8-9K-3 strain.
Keywords
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dahlen GG, Johnson JR, Gmur R. Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes, and binding characteristics. FEMS Microbiol. Lett. 138:89-95, 1996   DOI
2 Loesche WJ, Syed SA, Laughon BE, Stoll J. The bacteriology of acute necrotizing ulcerative gingivitis. J. Periodontol. 53:223-230, 1982   DOI
3 Premaraj T, Kato N, Fukui K, Kato H, Watanabe K. Use of PCR and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Techniques for Differentiation of Prevetella intermdeia Sensu Stricto and Prevotella nigrescens. J. Clin. Microbiol. 37:1057-1061, 1999
4 Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual' Ed, 2nd. Cold Spring Harbor Laboratory Press. 1988
5 Shah HN, S E Gharbia. Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int. J. Syst. Bacteriol. 42:542-546, 1992   DOI   ScienceOn
6 Walter JL. DNA probe and enzyme analysis in periodontal diagnostics. J. Periodontol. 63(12 Suppl):1102-1109, 1992   DOI   PUBMED
7 Frandsen EVG, Poulsen K, Kilian M. Confirmation of the species Prevotella intermedia and Prevotella nigrescens. Int. J. Syst. Bacteriol. 45:429-435, 1995   DOI   ScienceOn
8 Riggio MP, Lennon A, Roy KM. Detection of Prevotella intermedia in subgingival plaque of adult periodontitis patients by polymerase chain reaction. J. Periodontal Res. 33:369-376, 1998   DOI   ScienceOn
9 Moore WE, Holdeman LV, Smibert RM, et.al. Bacteriology of severe periodontitis in young adult humans. Infect. Immun. 38:1137-1148, 1982   PUBMED
10 Slots J. Subgingival microflora and periodontal disease. J. Clin. Periodontol. 6:351-382, 1979   DOI
11 Strzempko MN, Simon SL, French CK, et al. A Cross-reactivity Study of Whole Genomic DNA Probes for Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis.: J Dent Res. 1987;66(10): 1543-1546   DOI   ScienceOn
12 Nakagawa I, Amano A, Kimura RK, et.al. Distribution and molecular charaterization of Porphyromonas gingivalis carrying a new type of fimA gene. J. Clin. Microbiol. 38:1909-1914, 2000   PUBMED
13 Genco RJ, Loos BG. The use of genomic DNA fingerprinting in studies of the epidemiology of bacteria in periodontitis. J. Clin. Periodontol. 18:396-405, 1991   DOI
14 Tenenbaum H, Elkaim R, Cuisinier F, et.al. Prevalence of six periodontal pathogens detected by DNA probe method in HIV vs non-HIV periodontitis. Oral Dis. 3:S153-S155, 1997   DOI   PUBMED   ScienceOn
15 Conrads G, Mutters R, Fischer J, et.al. PCR reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally health individuals. J. Periodontol. 67:994-1003, 1996   DOI   PUBMED
16 Kook JK, Han JJ, Kim MK, et.al.. Reverse Dot Hybridization Method; A New Method for Rapid Screening of Bacterial Species-Specific DNA probe. Exp. Mol. Med. 33(3, Suppl):114, 2001
17 Moncla BJ, Strockbine L, Braham P, Karlinsey J, Roberts MC. The use of whole-cell DNA probes for the identification of Bacteroides intermedius isolates in a Dot Blot Assay. J. Dent. Res. 67:1267-1270, 1988   DOI   ScienceOn
18 Savitt ED, Strzempko MN, Vaccaro K, Peros WJ, French CK. Comparison of culture methods and DNA probe analyses for the detection of Actinobacillus actinomycetemconitans, Bacteroides gingivalis and Bacteroides intermedium in subgingival plaque samples. J. Periodontol. 59:431-438, 1988   DOI
19 Johnson JL, Holdeman LV. Bacteroides intermedius comb. nov. and descriptions of Bacteroides corporis sp. nov. and Bacteroides levii sp. nov. Int. J. Syst. Bacteriol. 33:15-25, 1983   DOI
20 Savitt ED, Keville MW, Peros WJ. DNA probes in the diagnosis of periodontal microorganisms. Arch. Ora.l Biol. 35:153s-159s, 1990   DOI   ScienceOn
21 Konoonen E, Pigmented Prevetella species in the periodontally healthy oral cavity. FEMS Immunol. Med. Microbiol. 6:201-206, 1993   DOI
22 Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent Jr RL. Microbial complexes in subgingival plaque. J. Clin. Periodontol. 25:134-144, 1998   DOI   ScienceOn
23 Morita M, Yamamoto T, Watanabe T. Identification by Biotinylated DNA Probes of Capnocytophaga Species Isolated from Supragingival Calculus. J. Dent. Res. 70:1048-1051. 1991   DOI   PUBMED   ScienceOn
24 Moore WEC, Moore LH, Ranney RR, et.al. The microflora of periodontal sites showing active destructive progression. J. Clin. Perodontol 18:729-739, 1991   DOI
25 Ashimoto A, Chen C, Bakker I, Slots, J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol. Immunol. 11;266-273, 1996   DOI   ScienceOn
26 Cookson AL, Wray A, Handley PS, Jacob AE. An investigation into the use of SDS-PAGE of cell surface extracts and proteolytic activity to differentiate Prevotella nigrescens and Prevotella intermedia. FEMS Microbiol. Lett. 136:109-115, 1996
27 Guillot E, C Mouton. PCR-DNA probe assays for identification and detection of Prevotella intermedia sensu stricto and Prevotella nigrescens. J. Clin. Microbiol. 35:1876-1882, 1997
28 Maidak BL, Olsen GJ, Larsen N, et.al. The ribosomal databse project (RDP). 24:82-85, 1996
29 Soder P-O, Jin LJ, Soder B. DNA probe detection of periodontopathogens in advanced periodontitis. Scand. J. Dent. Res. 101:363-70, 1993
30 Albandar JM, Brown LJ, Loe H. Putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis. J. Periodontol. 68:973-981, 1997   DOI