Subgingival microbiome in periodontitis and type 2 diabetes mellitus: an exploratory study using metagenomic sequencing |
Lu, Xianjun
(Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology)
Liu, Tingjun (Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology) Zhou, Jiani (Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology) Liu, Jia (Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology) Yuan, Zijian (Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology) Guo, Lihong (Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology) |
1 | Zhou M, Rong R, Munro D, Zhu C, Gao X, Zhang Q, et al. Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing. PLoS One 2013;8:e61516. |
2 | Kuntal BK, Chandrakar P, Sadhu S, Mande SS. 'NetShift': a methodology for understanding 'driver microbes' from healthy and disease microbiome datasets. ISME J 2019;13:442-54. DOI |
3 | Sakanaka A, Kuboniwa M, Hashino E, Bamba T, Fukusaki E, Amano A. Distinct signatures of dental plaque metabolic byproducts dictated by periodontal inflammatory status. Sci Rep 2017;7:42818. |
4 | Settem RP, El-Hassan AT, Honma K, Stafford GP, Sharma A. Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. Infect Immun 2012;80:2436-43. DOI |
5 | Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25:134-44. DOI |
6 | Preshaw PM, Alba AL, Herrera D, Jepsen S, Konstantinidis A, Makrilakis K, et al. Periodontitis and diabetes: a two-way relationship. Diabetologia 2012;55:21-31. DOI |
7 | Borrell LN, Kunzel C, Lamster I, Lalla E. Diabetes in the dental office: using NHANES III to estimate the probability of undiagnosed disease. J Periodontal Res 2007;42:559-65. DOI |
8 | Loos BG. Systemic markers of inflammation in periodontitis. J Periodontol 2005;76:2106-15. DOI |
9 | Rotter V, Nagaev I, Smith U. Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J Biol Chem 2003;278:45777-84. DOI |
10 | Salvi GE, Yalda B, Collins JG, Jones BH, Smith FW, Arnold RR, et al. Inflammatory mediator response as a potential risk marker for periodontal diseases in insulin-dependent diabetes mellitus patients. J Periodontol 1997;68:127-35. DOI |
11 | D'Aiuto F, Sabbah W, Netuveli G, Donos N, Hingorani AD, Deanfield J, et al. Association of the metabolic syndrome with severe periodontitis in a large U.S. population-based survey. J Clin Endocrinol Metab 2008;93:3989-94. DOI |
12 | Shi B, Lux R, Klokkevold P, Chang M, Barnard E, Haake S, et al. The subgingival microbiome associated with periodontitis in type 2 diabetes mellitus. ISME J 2020;14:519-30. DOI |
13 | Lamont RJ, Koo H, Hajishengallis G. The oral microbiota: dynamic communities and host interactions. Nat Rev Microbiol 2018;16:745-59. DOI |
14 | Holland IB. The extraordinary diversity of bacterial protein secretion mechanisms. Methods Mol Biol 2010;619:1-20. DOI |
15 | Bhavsar MV, Brahmbhatt NA, Sahayata V, Bhavsar NV. Gingival crevicular blood for screening of blood glucose level in patients with & without diabetes: a chair-side test. Int J Dent Hyg 2016;14:92-7. DOI |
16 | Pfannkuch L, Hurwitz R, Traulsen J, Sigulla J, Poeschke M, Matzner L, et al. ADP heptose, a novel pathogen-associated molecular pattern identified in Helicobacter pylori. FASEB J 2019;33:9087-99. DOI |
17 | Gray LR, Tompkins SC, Taylor EB. Regulation of pyruvate metabolism and human disease. Cell Mol Life Sci 2014;71:2577-604. DOI |
18 | Gao Z, Yin J, Zhang J, Ward RE, Martin RJ, Lefevre M, et al. Butyrate improves insulin sensitivity and increases energy expenditure in mice. Diabetes 2009;58:1509-17. DOI |
19 | Rathinam VA, Zhao Y, Shao F. Innate immunity to intracellular LPS. Nat Immunol 2019;20:527-33. DOI |
20 | Pandolfi F, Altamura S, Frosali S, Conti P. Key role of DAMP in inflammation, cancer, and tissue repair. Clin Ther 2016;38:1017-28. DOI |
21 | Green ER, Mecsas J. Bacterial secretion systems: an overview. Microbiol Spectr 2016;4:4.1.13. |
22 | Heinz E, Selkrig J, Belousoff MJ, Lithgow T. Evolution of the translocation and assembly module (TAM). Genome Biol Evol 2015;7:1628-43. DOI |
23 | Torresyap G, Haffajee AD, Uzel NG, Socransky SS. Relationship between periodontal pocket sulfide levels and subgingival species. J Clin Periodontol 2003;30:1003-10. DOI |
24 | Wang S, Heng BC, Qiu S, Deng J, Shun Pan Cheung G, Jin L, et al. Lipoteichoic acid of Enterococcus faecalis inhibits osteoclastogenesis via transcription factor RBP-J. Innate Immun 2019;25:13-21. DOI |
25 | Jung S, Park OJ, Kim AR, Ahn KB, Lee D, Kum KY, et al. Lipoteichoic acids of lactobacilli inhibit Enterococcus faecalis biofilm formation and disrupt the preformed biofilm. J Microbiol 2019;57:310-5. DOI |
26 | Lee JC, Stewart GC. Essential nature of the mreC determinant of Bacillus subtilis. J Bacteriol 2003;185:4490-8. DOI |
27 | Anderson GG, Yahr TL, Lovewell RR, O'Toole GA. The Pseudomonas aeruginosa magnesium transporter mgtE inhibits transcription of the type III secretion system. Infect Immun 2010;78:1239-49. DOI |
28 | Okamoto M, Maeda N, Kondo K, Leung KP. Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens. FEMS Microbiol Lett 1999;178:299-304. DOI |
29 | Leung K, Nesbitt WE, Okamoto M, Fukushima H. Identification of a fimbriae-associated haemagglutinin from Prevotella intermedia. Microb Pathog 1999;26:139-48. DOI |
30 | Kesavalu L, Holt SC, Ebersole JL. In vitro environmental regulation of Porphyromonas gingivalis growth and virulence. Oral Microbiol Immunol 2003;18:226-33. DOI |
31 | Coffey BM, Akhand SS, Anderson GG. MgtE is a dual-function protein in Pseudomonas aeruginosa. Microbiology (Reading) 2014;160:1200-13. DOI |
32 | Chi XP, Ouyang XY, Wang YX. Hydrogen sulfide synergistically upregulates Porphyromonas gingivalis lipopolysaccharide-induced expression of IL-6 and IL-8 via NF-κB signalling in periodontal fibroblasts. Arch Oral Biol 2014;59:954-61. DOI |
33 | Hajishengallis G, Darveau RP, Curtis MA. The keystone-pathogen hypothesis. Nat Rev Microbiol 2012;10:717-25. DOI |
34 | Reyes-Soffer G, Ginsberg HN, Ramakrishnan R. The metabolism of lipoprotein (a): an ever-evolving story. J Lipid Res 2017;58:1756-64. DOI |
35 | Shinagawa H, Shiba T, Iwasaki H, Makino K, Takahagi M, Nakata A. Properties of the Escherichia coli RuvA and RuvB proteins involved in DNA repair, recombination and mutagenesis. Biochimie 1991;73:505-7. DOI |
36 | Estevao S, Sluijter M, Hartwig NG, van Rossum AM, Vink C. Functional characterization of the RuvB homologs from Mycoplasma pneumoniae and Mycoplasma genitalium. J Bacteriol 2011;193:6425-35. DOI |
37 | Farina R, Severi M, Carrieri A, Miotto E, Sabbioni S, Trombelli L, et al. Whole metagenomic shotgun sequencing of the subgingival microbiome of diabetics and non-diabetics with different periodontal conditions. Arch Oral Biol 2019;104:13-23. DOI |
38 | Taylor JJ, Preshaw PM, Lalla E. A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes. J Clin Periodontol 2013;40 Suppl 14:S113-34. DOI |
39 | Sultana ST, Call DR, Beyenal H. Eradication of Pseudomonas aeruginosa biofilms and persister cells using an electrochemical scaffold and enhanced antibiotic susceptibility. NPJ Biofilms Microbiomes 2016;2:2. |
40 | Hsaine S, Fethi FZ, Charof R, Ounine K. Microbiological study of oral flora in diabetic patients with gingivitis. Int J Pharm Pharm Sci 2018;10:113. |
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