Browse > Article
http://dx.doi.org/10.5051/jpis.2016.46.6.362

Biocompatibility study of lithium disilicate and zirconium oxide ceramics for esthetic dental abutments  

Brunot-Gohin, Celine (Laboratory of Biomaterials and Bone Site Inflammation, University of Reims Champagne-Ardenne)
Duval, Jean-Luc (Laboratory of Biomechanics and Bioengineering, Research Center of Royallieu, University of Technology of Compiegne, Sorbonne Universities)
Verbeke, Sandra (University of Reims Champagne-Ardenne, Faculty of Odontology)
Belanger, Kayla (Laboratory of Biomechanics and Bioengineering, Research Center of Royallieu, University of Technology of Compiegne, Sorbonne Universities)
Pezron, Isabelle (Laboratory of Integrated Renewable Matter Transformations, Research Center of Royallieu, University of Technology of Compiegne, Sorbonne Universities)
Kugel, Gerard (Tufts University School of Dental Medicine)
Laurent-Maquin, Dominique (Laboratory of Biomaterials and Bone Site Inflammation, University of Reims Champagne-Ardenne)
Gangloff, Sophie (Laboratory of Biomaterials and Bone Site Inflammation, University of Reims Champagne-Ardenne)
Egles, Christophe (Laboratory of Biomechanics and Bioengineering, Research Center of Royallieu, University of Technology of Compiegne, Sorbonne Universities)
Publication Information
Journal of Periodontal and Implant Science / v.46, no.6, 2016 , pp. 362-371 More about this Journal
Abstract
Purpose: The increasing demand for esthetically pleasing results has contributed to the use of ceramics for dental implant abutments. The aim of this study was to compare the biological response of epithelial tissue cultivated on lithium disilicate ($LS_2$) and zirconium oxide ($ZrO_2$) ceramics. Understanding the relevant physicochemical and mechanical properties of these ceramics will help identify the optimal material for facilitating gingival wound closure. Methods: Both biomaterials were prepared with 2 different surface treatments: raw and polished. Their physicochemical characteristics were analyzed by contact angle measurements, scanning white-light interferometry, and scanning electron microscopy. An organotypic culture was then performed using a chicken epithelium model to simulate peri-implant soft tissue. We measured the contact angle, hydrophobicity, and roughness of the materials as well as the tissue behavior at their surfaces (cell migration and cell adhesion). Results: The best cell migration was observed on $ZrO_2$ ceramic. Cell adhesion was also drastically lower on the polished $ZrO_2$ ceramic than on both the raw and polished $LS_2$. Evaluating various surface topographies of $LS_2$ showed that increasing surface roughness improved cell adhesion, leading to an increase of up to 13%. Conclusions: Our results demonstrate that a biomaterial, here $LS_2$, can be modified using simple surface changes in order to finely modulate soft tissue adhesion. Strong adhesion at the abutment associated with weak migration assists in gingival wound healing. On the same material, polishing can reduce cell adhesion without drastically modifying cell migration. A comparison of $LS_2$ and $ZrO_2$ ceramic showed that $LS_2$ was more conducive to creating varying tissue reactions. Our results can help dental surgeons to choose, especially for esthetic implant abutments, the most appropriate biomaterial as well as the most appropriate surface treatment to use in accordance with specific clinical dental applications.
Keywords
Ceramics; Dental abutments; Dental esthetics; Embryo culture techniques;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bressan E, Paniz G, Lops D, Corazza B, Romeo E, Favero G. Influence of abutment material on the gingival color of implant-supported all-ceramic restorations: a prospective multicenter study. Clin Oral Implants Res 2011;22:631-7.   DOI
2 Rajaraman R, Rounds DE, Yen SP, Rembaum A. A scanning electron microscope study of cell adhesion and spreading in vitro. Exp Cell Res 1974;88:327-39.   DOI
3 Duval JL, Letort M, Sigot-Luizard MF. Comparative assessment of cell/substratum static adhesion using an in vitro organ culture method and computerized analysis system. Biomaterials 1988;9:155-61.   DOI
4 Sigot-Luizard MF, Lanfranchi M, Duval JL, Benslimane S, Sigot M, Guidoin RG, et al. The cytocompatibility of compound polyester-protein surfaces using an in vitro technique. In Vitro Cell Dev Biol 1986;22:234-40.   DOI
5 Abramoff MD, Magalhaes PJ, Ram SJ. Image processing with ImageJ. Biophotonics Int 2004;11:36-42.
6 Josset Y, Oum'Hamed Z, Zarrinpour A, Lorenzato M, Adnet JJ, Laurent-Maquin D. In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics. J Biomed Mater Res 1999;47:481-93.   DOI
7 Covacci V, Bruzzese N, Maccauro G, Andreassi C, Ricci GA, Piconi C, et al. In vitro evaluation of the mutagenic and carcinogenic power of high purity zirconia ceramic. Biomaterials 1999;20:371-6.   DOI
8 Warashina H, Sakano S, Kitamura S, Yamauchi KI, Yamaguchi J, Ishiguro N, et al. Biological reaction to alumina, zirconia, titanium and polyethylene particles implanted onto murine calvaria. Biomaterials 2003;24:3655-61.   DOI
9 van Brakel R, Meijer GJ, Verhoeven JW, Jansen J, de Putter C, Cune MS. Soft tissue response to zirconia and titanium implant abutments: an in vivo within-subject comparison. J Clin Periodontol 2012;39:995-1001.   DOI
10 De Sanctis M, Baldini N, Vignoletti F. Soft tissue management around teeth and implants. J Parodontol Implantol Oral 2010;29:245-69.
11 Rimondini L, Cerroni L, Carrassi A, Torricelli P. Bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study. Int J Oral Maxillofac Implants 2002;17:793-8.
12 Brunot-Gohin C, Duval JL, Azogui EE, Jannetta R, Pezron I, Laurent-Maquin D, et al. Soft tissue adhesion of polished versus glazed lithium disilicate ceramic for dental applications. Dent Mater 2013;29:e205-12.   DOI
13 Muller C, Luders A, Hoth-Hannig W, Hannig M, Ziegler C. Initial bioadhesion on dental materials as a function of contact time, pH, surface wettability, and isoelectric point. Langmuir 2010;26:4136-41.   DOI
14 Brackett MG, Lockwood PE, Messer RL, Lewis JB, Bouillaguet S, Wataha JC. In vitro cytotoxic response to lithium disilicate dental ceramics. Dent Mater 2008;24:450-6.   DOI
15 Ekfeldt A, Furst B, Carlsson GE. Zirconia abutments for single-tooth implant restorations: a retrospective and clinical follow-up study. Clin Oral Implants Res 2011;22:1308-14.   DOI
16 Grunder U, Gracis S, Capelli M. Influence of the 3-D bone-to-implant relationship on esthetics. Int J Periodontics Restorative Dent 2005;25:113-9.
17 Bolortuya G, Ebihara A, Ichinose S, Watanabe S, Anjo T, Kokuzawa C, et al. Effects of dentin surface modifications treated with Er:YAG and Nd:YAG laser irradiation on fibroblast cell adhesion. Photomed Laser Surg 2012;30:63-70.   DOI
18 Duval JL, Dinis T, Vidal G, Vigneron P, Kaplan DL, Egles C. Organotypic culture to assess cell adhesion, growth and alignment of different organs on silk fibroin. J Tissue Eng Regen Med. Forthcoming 2014.
19 Messer RL, Lockwood PE, Wataha JC, Lewis JB, Norris S, Bouillaguet S. In vitro cytotoxicity of traditional versus contemporary dental ceramics. J Prosthet Dent 2003;90:452-8.   DOI
20 Scarano A, Piattelli M, Caputi S, Favero GA, Piattelli A. Bacterial adhesion on commercially pure titanium and zirconium oxide disks: an in vivo human study. J Periodontol 2004;75:292-6.   DOI
21 Rompen E, Raepsaet N, Domken O, Touati B, Van Dooren E. Soft tissue stability at the facial aspect of gingivally converging abutments in the esthetic zone: a pilot clinical study. J Prosthet Dent 2007;97:S119-25.   DOI
22 Grundke K, Bogumil T, Werner C, Janke A, Poschel K, Jacobasch HJ. Liquid-fluid contact angle measurements on hydrophilic cellulosic materials. Colloids Surf A Physicochem Eng Asp 1996;116:79-91.   DOI
23 Guess PC, Zavanelli RA, Silva NR, Bonfante EA, Coelho PG, Thompson VP. Monolithic CAD/CAM lithium disilicate versus veneered Y-TZP crowns: comparison of failure modes and reliability after fatigue. Int J Prosthodont 2010;23:434-42.
24 Van Dooren E, Calamita M, Calgaro M, Coachman C, Ferencz JL, Pinho C, et al. Mechanical, biological and clinical aspects of zirconia implants. Eur J Esthet Dent 2012;7:396-417.
25 Wittneben JG, Wright RF, Weber HP, Gallucci GO. A systematic review of the clinical performance of CAD/CAM single-tooth restorations. Int J Prosthodont 2009;22:466-71.
26 de Groot P, Deck L. Surface profiling by frequency-domain analysis of white light interferograms. Proc SPIE 1994;2248:101-4.
27 Valenti M, Valenti A. Retrospective survival analysis of 261 lithium disilicate crowns in a private general practice. Quintessence Int 2009;40:573-9.
28 Gehrt M, Wolfart S, Rafai N, Reich S, Edelhoff D. Clinical results of lithium-disilicate crowns after up to 9 years of service. Clin Oral Investig 2013;17:275-84.   DOI
29 Herrguth M, Wichmann M, Reich S. The aesthetics of all-ceramic veneered and monolithic CAD/CAM crowns. J Oral Rehabil 2005;32:747-52.   DOI
30 Bengazi F, Wennstrom JL, Lekholm U. Recession of the soft tissue margin at oral implants. A 2-year longitudinal prospective study. Clin Oral Implants Res 1996;7:303-10.   DOI