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http://dx.doi.org/10.5051/jpis.2011.41.5.218

The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects  

Park, Shin-Young (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Kim, Kyoung-Hwa (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Koo, Ki-Tae (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Lee, Kang-Woon (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Lee, Yong-Moo (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Chung, Chong-Pyoung (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Seol, Yang-Jo (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.41, no.5, 2011 , pp. 218-226 More about this Journal
Abstract
Purpose: Micro-computed tomography (micro-CT) has been widely used in the evaluation of regenerated bone tissue but the reliability of micro-CT has not yet been established. This study evaluated the correlation between histomorphometric analysis and micro-CT analysis in performing new bone formation measurement. Methods: Critical-size calvarial defects were created using a 8 mm trephine bur in a total of 24 Sprague-Dawley rats, and collagen gel mixed with autogenous rat bone marrow stromal cells (BMSCs) or autogenous rat BMSCs transduced by adenovirus containing bone morphogenic protein-2 (BMP-2) genes was loaded into the defect site. In the control group, collagen gel alone was loaded into the defect. After 2 and 4 weeks, the animals were euthanized and calvaria containing defects were harvested. Micro-CT analysis and histomorphometric analysis of each sample were accomplished and the statistical evaluation about the correlation between both analyses was performed. Results: New bone formation of the BMP-2 group was greater than that of the other groups at 2 and 4 weeks in both histomorphometric analysis and micro-CT analysis (P=0.026, P=0.034). Histomorphometric analysis of representative sections showed similar results to histomorphometric analysis with a mean value of 3 sections. Measurement of new bone formation was highly correlated between histomorphometric analysis and micro-CT analysis, especially at the low lower threshold level at 2 weeks (adjusted $r^2=0.907$, P<0.001). New bone formation of the BMP-2 group analyzed by micro-CT tended to decline sharply with an increasing lower threshold level, and it was statistically significant (P<0.001). Conclusions: Both histomorphometric analysis and micro-CT analysis were valid methods for measurement of the new bone in rat calvarial defects and the ability to detect the new bone in micro-CT analysis was highly influenced by the threshold level in the BMP-2 group at early stage.
Keywords
Bone morphogenic protein-2; Gene therapy; Histology; X-Ray microtomography;
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