Browse > Article
http://dx.doi.org/10.5051/jpis.2011.41.1.30

Analysis of gene expression during mineralization of cultured human periodontal ligament cells  

Choi, Hee-Dong (Department of Periodontology, Kyungpook National University School of Dentistry)
Noh, Woo-Chang (Department of Periodontology, Kyungpook National University School of Dentistry)
Park, Jin-Woo (Department of Periodontology, Kyungpook National University School of Dentistry)
Lee, Jae-Mok (Department of Periodontology, Kyungpook National University School of Dentistry)
Suh, Jo-Young (Department of Periodontology, Kyungpook National University School of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.41, no.1, 2011 , pp. 30-43 More about this Journal
Abstract
Purpose: Under different culture conditions, periodontal ligament (PDL) stem cells are capable of differentiating into cementoblast-like cells, adipocytes, and collagen-forming cells. Several previous studies reported that because of the stem cells in the PDL, the PDL have a regenerative capacity which, when appropriately triggered, participates in restoring connective tissues and mineralized tissues. Therefore, this study analyzed the genes involved in mineralization during differentiation of human PDL (hPDL) cells, and searched for candidate genes possibly associated with the mineralization of hPDL cells. Methods: To analyze the gene expression pattern of hPDL cells during differentiation, the hPDL cells were cultured in two conditions, with or without osteogenic cocktails (${\beta}$-glycerophosphate, ascorbic acid and dexamethasone), and a DNA microarray analysis of the cells cultured on days 7 and 14 was performed. Reverse transcription-polymerase chain reaction was performed to validate the DNA microarray data. Results: The up-regulated genes on day 7 by hPDL cells cultured in osteogenic medium were thought to be associated with calcium/iron/metal ion binding or homeostasis (PDE1A, HFE and PCDH9) and cell viability (PCDH9), and the down-regulated genes were thought to be associated with proliferation (PHGDH and PSAT1). Also, the up-regulated genes on day 14 by hPDL cells cultured in osteogenic medium were thought to be associated with apoptosis, angiogenesis (ANGPTL4 and FOXO1A), and adipogenesis (ANGPTL4 and SEC14L2), and the down-regulated genes were thought to be associated with cell migration (SLC16A4). Conclusions: This study suggests that when appropriately triggered, the stem cells in the hPDL differentiate into osteoblasts/cementoblasts, and the genes related to calcium binding (PDE1A and PCDH9), which were strongly expressed at the stage of matrix maturation, may be associated with differentiation of the hPDL cells into osteoblasts/cementoblasts.
Keywords
Microarray analysis; Gene expression profiling; Periodontal ligament; Cell differentiation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Kobayashi M, Takiguchi T, Suzuki R, Yamaguchi A, Deguchi K, Shionome M, et al. Recombinant human bone morphogenetic protein-2 stimulates osteoblastic differentiation in cells isolated from human periodontal ligament. J Dent Res 1999;78:1624-33.   DOI   ScienceOn
2 Kitagawa M, Tahara H, Kitagawa S, Oka H, Kudo Y, Sato S, et al. Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo. Bone 2006;39:1035-42.   DOI   ScienceOn
3 Iwata T, Yamato M, Tsuchioka H, Takagi R, Mukobata S, Washio K, et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials 2009;30:2716-23.   DOI   ScienceOn
4 Kulterer B, Friedl G, Jandrositz A, Sanchez-Cabo F, Prokesch A, Paar C, et al. Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation. BMC Genomics 2007;8:70.   DOI   ScienceOn
5 Yamada S, Ozawa Y, Tomoeda M, Matoba R, Matsubara K, Murakami S. Regulation of PLAP-1 expression in periodontal ligament cells. J Dent Res 2006;85:447-51.   DOI   ScienceOn
6 Lallier TE, Spencer A. Use of microarrays to find novel regulators of periodontal ligament fibroblast differentiation. Cell Tissue Res 2007;327:93-109.
7 Shin JH, Park JW, Yeo SI, Noh WC, Kim MK, Kim JC, et al. Identification of matrix mineralization-related genes in human periodontal ligament cells using cDNA microarray. J Korean Acad Periodontol 2007;37(Suppl):447-63.
8 Dahl LK. A simple and sensitive histochemical method for calcium. Proc Soc Exp Biol Med 1952;80:474-9.   DOI
9 Lekic P, Rojas J, Birek C, Tenenbaum H, McCulloch CA. Phenotypic comparison of periodontal ligament cells in vivo and in vitro. J Periodontal Res 2001;36:71-9.   DOI   ScienceOn
10 Ivanovski S, Haase HR, Bartold PM. Expression of bone matrix protein mRNAs by primary and cloned cultures of the regenerative phenotype of human periodontal fibroblasts. J Dent Res 2001;80:1665-71.   DOI   ScienceOn
11 Murakami Y, Kojima T, Nagasawa T, Kobayashi H, Ishikawa I. Novel isolation of alkaline phosphatase-positive subpopulation from periodontal ligament fibroblasts. J Periodontol 2003;74:780-6.   DOI   ScienceOn
12 Wu L, Wei X, Ling J, Liu L, Liu S, Li M, et al. Early osteogenic differential protein profile detected by proteomic analysis in human periodontal ligament cells. J Periodontal Res 2009;44:645-56.   DOI   ScienceOn
13 Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149-55.   DOI   ScienceOn
14 Flores MG, Yashiro R, Washio K, Yamato M, Okano T, Ishikawa I. Periodontal ligament cell sheet promotes periodontal regeneration in athymic rats. J Clin Periodontol 2008;35:1066-72.   DOI   ScienceOn
15 Michibata H, Yanaka N, Kanoh Y, Okumura K, Omori K. Human Ca2+/calmodulin-dependent phosphodiesterase $PDE_1A$: novel splice variants, their specific expression, genomic organization, and chromosomal localization. Biochim Biophys Acta 2001;1517:278-87.   DOI   ScienceOn
16 Gray MJ, Van Buren G, Dallas NA, Xia L, Wang X, Yang AD, et al. Therapeutic targeting of neuropilin-2 on colorectal carcinoma cells implanted in the murine liver. J Natl Cancer Inst 2008;100:109-20.   DOI   ScienceOn
17 Hopwood B, Tsykin A, Findlay DM, Fazzalari NL. Gene expression profile of the bone microenvironment in human fragility fracture bone. Bone 2009;44:87-101.   DOI   ScienceOn
18 Cho HM, Jun DY, Bae MA, Ahn JD, Kim YH. Nucleotide sequence and differential expression of the human 3-phosphoglycerate dehydrogenase gene. Gene 2000;245:193-201.   DOI   ScienceOn
19 Pollari S, Kakonen SM, Edgren H, Wolf M, Kohonen P, Sara H, et al. Enhanced serine production by bone metastatic breast cancer cells stimulates osteoclastogenesis. Breast Cancer Res Treat 2011;125:421-30.   DOI
20 Gallagher SM, Castorino JJ, Philp NJ. Interaction of monocarboxylate transporter 4 with beta1-integrin and its role in cell migration. Am J Physiol Cell Physiol 2009;296:C414-21.   DOI
21 Bartold PM, Shi S, Gronthos S. Stem cells and periodontal regeneration. Periodontol 2000 2006;40:164-72.   DOI   ScienceOn
22 Fidock M, Miller M, Lanfear J. Isolation and differential tissue distribution of two human cDNAs encoding $PDE_1A$ splice variants. Cell Signal 2002;14:53-60.   DOI   ScienceOn
23 Zayzafoon M, Fulzele K, McDonald JM. Calmodulin and calmodulin-dependent kinase IIalpha regulate osteoblast differentiation by controlling c-fos expression. J Biol Chem 2005;280:7049-59.   DOI
24 Etzrodt J, Krishna-K K, Redies C. Expression of classic cadherins and delta-protocadherins in the developing ferret retina. BMC Neurosci 2009;10:153.   DOI
25 Nagatomo K, Komaki M, Sekiya I, Sakaguchi Y, Noguchi K, Oda S, et al. Stem cell properties of human periodontal ligament cells. J Periodontal Res 2006;41:303-10.   DOI   ScienceOn