Browse > Article
http://dx.doi.org/10.5051/jpis.2010.40.5.232

Biological effects of a porcine-derived collagen membrane on intrabony defects  

Lee, Chang-Kyun (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Koo, Ki-Tae (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Kim, Tae-Il (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Seol, Yang-Jo (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Lee, Yong-Moo (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Rhyu, In-Chul (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Ku, Young (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Chung, Chong-Pyoung (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
Park, Yoon-Jeong (Department of Craniomaxillofacial Reconstructive Sciences, Seoul National University School of Dentistry)
Lee, Jue-Yeon (Research Center, Nano Intelligent Biomedical Engineering Corporation)
Publication Information
Journal of Periodontal and Implant Science / v.40, no.5, 2010 , pp. 232-238 More about this Journal
Abstract
Purpose: To prolong the degradation time of collagen membranes, various cross-linking techniques have been developed. For cross-linking, chemicals such as formaldehyde and glutaraldehyde are added to collagen membranes, but these chemicals could adversely affect surrounding tissues. The aim of this study is to evaluate the ability of porous non-chemical cross-linking porcine-derived collagen nanofibrous membrane to enhance bone and associated tissue regeneration in one-wall intrabony defects in beagle dogs. Methods: The second and third mandibular premolars and the first molars of 2 adult beagles were extracted bilaterally and the extraction sites were allowed to heal for 10 weeks. One-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Among eight defects, four defects were not covered with membrane as controls and the other four defects were covered with membrane as the experimental group. The animals were sacrificed 10 weeks after surgery. Results: Wound healing was generally uneventful. For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control. In new bone height (NBh), the experimental group exhibited a greater mean value than the control ($3.04{\pm}0.23\;mm/1.57{\pm}0.59$, P=0.003). Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, $34.48{\pm}10.21%$ vs. $5.09{\pm}5.76%$, P=0.014; and NBv, $28.04{\pm}12.96$ vs. $1.55{\pm}0.57$, P=0.041). On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups. Conclusions: Within the limitations of this study, this collagen membrane enhanced bone regeneration at one-wall intrabony defects. On the other hand, no influence of this membrane on periodontal tissue regeneration could be ascertained in this study.
Keywords
Absorbable implants; Bone regeneration; Collagen; Guided tissue regeneration;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kochi G, Sato S, Ebihara H, Hirano J, Arai Y, Ito K. A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium. J Oral Sci 2010;52:203-11.   DOI   ScienceOn
2 Yeo YJ, Jeon DW, Kim CS, Choi SH, Cho KS, Lee YK, et al. Effects of chitosan nonwoven membrane on periodontal healing of surgically created one-wall intrabony defects in beagle dogs. J Biomed Mater Res B Appl Biomater 2005;72: 86-93.
3 Stoller NH, Johnson LR, Garrett S. Periodontal regeneration of a class II furcation defect utilizing a bioabsorbable barrier in a human. A case study with histology. J Periodontol 2001;72:238-42.   DOI   ScienceOn
4 Sculean A, Donos N, Chiantella GC, Windisch P, Reich E, Brecx M. GTR with bioresorbable membranes in the treatment of intrabony defects: a clinical and histologic study. Int J Periodontics Restorative Dent 1999;19:501-9.
5 Sculean A, Donos N, Windisch P, Brecx M, Gera I, Reich E, et al. Healing of human intrabony defects following treatment with enamel matrix proteins or guided tissue regeneration. J Periodontal Res 1999;34:310-22.   DOI
6 Muller R, Hahn M, Vogel M, Delling G, Ruegsegger P. Morphometric analysis of noninvasively assessed bone biopsies: comparison of high-resolution computed tomography and histologic sections. Bone 1996;18:215-20.   DOI   ScienceOn
7 Butz F, Ogawa T, Chang TL, Nishimura I. Three-dimensional bone-implant integration profiling using microcomputed tomography. Int J Oral Maxillofac Implants 2006;21:687-95.
8 Cowan CM, Aghaloo T, Chou YF, Walder B, Zhang X, Soo C, et al. MicroCT evaluation of three-dimensional mineralization in response to BMP-2 doses in vitro and in critical sized rat calvarial defects. Tissue Eng 2007;13:501-12.   DOI   ScienceOn
9 Marechal M, Luyten F, Nijs J, Postnov A, Schepers E, van Steenberghe D. Histomorphometry and micro-computed tomography of bone augmentation under a titanium membrane. Clin Oral Implants Res 2005;16:708-14.   DOI   ScienceOn
10 Minabe M, Kodama T, Kogou T, Tamura T, Hori T, Watanabe Y, et al. Different cross-linked types of collagen implanted in rat palatal gingiva. J Periodontol 1989;60:35-43.   DOI
11 Speer DP, Chvapil M, Eskelson CD, Ulreich J. Biological effects of residual glutaraldehyde in glutaraldehyde-tanned collagen biomaterials. J Biomed Mater Res 1980;14:753-64.   DOI   ScienceOn
12 Kim CS, Choi SH, Chai JK, Cho KS, Moon IS, Wikesjo UM, et al. Periodontal repair in surgically created intrabony defects in dogs: influence of the number of bone walls on healing response. J Periodontol 2004;75:229-35.   DOI   ScienceOn
13 Wikesjo UM, Lim WH, Razi SS, Sigurdsson TJ, Lee MB, Tatakis DN, et al. Periodontal repair in dogs: a bioabsorbable calcium carbonate coral implant enhances space provision for alveolar bone regeneration in conjunction with guided tissue regeneration. J Periodontol 2003;74:957-64.   DOI   ScienceOn
14 Blumenthal NM, Alves ME, Al-Huwais S, Hofbauer AM, Koperski RD. Defect-determined regenerative options for treating periodontal intrabony defects in baboons. J Periodontol 2003;74:10-24.   DOI   ScienceOn
15 Schliephake H, Dard M, Planck H, Hierlemann H, Jakob A. Guided bone regeneration around endosseous implants using a resorbable membrane vs a PTFE membrane. Clin Oral Implants Res 2000;11:230-41.   DOI   ScienceOn
16 Locci P, Calvitti M, Belcastro S, Pugliese M, Guerra M, Marinucci L, et al. Phenotype expression of gingival fibroblasts cultured on membranes used in guided tissue regeneration. J Periodontol 1997;68:857-63.   DOI   ScienceOn
17 Polimeni G, Koo KT, Qahash M, Xiropaidis AV, Albandar JM, Wikesjo UM. Prognostic factors for alveolar regeneration: effect of a space-providing biomaterial on guided tissue regeneration. J Clin Periodontol 2004;31:725-9.   DOI   ScienceOn
18 Ling LJ, Hung SL, Lee CF, Chen YT, Wu KM. The influence of membrane exposure on the outcomes of guided tissue regeneration: clinical and microbiological aspects. J Periodontal Res 2003;38:57-63.   DOI   ScienceOn
19 Parrish LC, Miyamoto T, Fong N, Mattson JS, Cerutis DR. Non-bioabsorbable vs. bioabsorbable membrane: assessment of their clinical efficacy in guided tissue regeneration technique. A systematic review. J Oral Sci 2009;51:383-400.   DOI   ScienceOn
20 Quteish D, Singrao S, Dolby AE. Light and electron microscopic evaluation of biocompatibility, resorption and penetration characteristics of human collagen graft material. J Clin Periodontol 1991;18:305-11.   DOI
21 Garrett S. Periodontal regeneration around natural teeth. Ann Periodontol 1996;1:621-66.   DOI   ScienceOn
22 Caton J, Nyman S, Zander H. Histometric evaluation of periodontal surgery. II. Connective tissue attachment levels after four regenerative procedures. J Clin Periodontol 1980;7:224-31.   DOI
23 Bowers GM, Chadroff B, Carnevale R, Mellonig J, Corio R, Emerson J, et al. Histologic evaluation of new attachment apparatus formation in humans. Part I. J Periodontol 1989; 60:664-74.   DOI
24 Gottlow J, Nyman S, Karring T, Lindhe J. New attachment formation as the result of controlled tissue regeneration. J Clin Periodontol 1984;11:494-503.   DOI
25 Buser D, Dula K, Belser U, Hirt HP, Berthold H. Localized ridge augmentation using guided bone regeneration. 1. Surgical procedure in the maxilla. Int J Periodontics Restorative Dent 1993;13:29-45.
26 Dahlin C, Sennerby L, Lekholm U, Linde A, Nyman S. Generation of new bone around titanium implants using a membrane technique: an experimental study in rabbits. Int J Oral Maxillofac Implants 1989;4:19-25.
27 Simion M, Scarano A, Gionso L, Piattelli A. Guided bone regeneration using resorbable and nonresorbable mem branes: a comparative histologic study in humans. Int J Oral Maxillofac Implants 1996;11:735-42.
28 Becker W, Becker BE, Caffesse R, Kerry G, Ochsenbein C, Morrison E, et al. A longitudinal study comparing scaling, osseous surgery, and modified Widman procedures: results after 5 years. J Periodontol 2001;72:1675-84.   DOI   ScienceOn
29 Ramfjord SP, Knowles JW, Nissle RR, Burgett FG, Shick RA. Results following three modalities of periodontal therapy. J Periodontol 1975;46:522-6.   DOI
30 Kaldahl WB, Kalkwarf KL, Patil KD, Molvar MP, Dyer JK. Long-term evaluation of periodontal therapy: I. Response to 4 therapeutic modalities. J Periodontol 1996;67:93-102.   DOI
31 Caton J, Nyman S. Histometric evaluation of periodontal surgery. I. The modified Widman flap procedure. J Clin Periodontol 1980;7:212-23.   DOI
32 Song WS, Kim CS, Choi SH, Jhon GJ, Kim HY, Cho KS, et al. The effects of a bioabsorbable barrier membrane containing safflower seed extracts on periodontal healing of 1-wall intrabony defects in beagle dogs. J Periodontol 2005;76:22-33.   DOI   ScienceOn
33 Polimeni G, Koo KT, Pringle GA, Agelan A, Safadi FF, Wikesjo UM. Histopathological observations of a polylactic acid- based device intended for guided bone/tissue regeneration. Clin Implant Dent Relat Res 2008;10:99-105.   DOI   ScienceOn
34 Sculean A, Nikolidakis D, Schwarz F. Regeneration of periodontal tissues: combinations of barrier membranes and grafting materials - biological foundation and preclinical evidence: a systematic review. J Clin Periodontol 2008;35: 106-16.   DOI   ScienceOn