Browse > Article
http://dx.doi.org/10.5051/jpis.2010.40.3.111

Evaluation of vitrification for cryopreservation of teeth  

Dissanayake, Surangi C. (Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry)
Che, Zhong-Min (Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry)
Choi, Seong-Ho (Department of Periodontology, Yonsei University College of Dentistry)
Lee, Seung-Jong (Department of Conservative Dentistry, Yonsei University College of Dentistry)
Kim, Jin (Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.40, no.3, 2010 , pp. 111-118 More about this Journal
Abstract
Purpose: The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. Methods: In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified vitrification formulae named T1 (75% vitrification media + 25% F-media), T2 (50% vitrification media + 50% F-media) and T3 (25% vitrification media + 75% F-media) were used to store PDL cells for 2 weeks and 4 weeks in liquid nitrogen. MTT assay was performed to examine the viability of PDL cells. Results: Maximum cell viability was achieved in cells stored in 100% conventional cryopreservation media at $-196^{\circ}C$ (positive control group) in step 1. Compared to the positive control group, viability of the cells stored in 100% vitrification media was very low as 10% in all test conditions. In step 2, as the percentage of vitrification media decreased, the cell viability increased in cells stored for 2 weeks. In 4-week storage of cells in step 2, higher cell viability was observed in the T2 group than the other vitrification formulae while the positive control group had the highest viability. There was no statistically significant difference in the cell viability of 2-week and 4-week stored cells in the T2 group. Conclusions: These observations indicate 100% vitrification media is not successful in PDL cell cryopreservation. Conventional cryopreservation media is currently the most appropriate media type for this purpose while T2 media would be interesting to test for long-term storage of PDL cells.
Keywords
Cryopreservation; Periodontal ligament; Tissue banks;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Isachenko V, Lapidus I, Isachenko E, Krivokharchenko A, Kreienberg R, Woriedh M, et al. Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation. Reproduction 2009;138:319-27.   DOI   ScienceOn
2 Temmerman L, De Pauw GA, Beele H, Dermaut LR. Tooth transplantation and cryopreservation: state of the art. Am J Orthod Dentofacial Orthop 2006;129:691-5.   DOI   ScienceOn
3 Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990;89:91-7.   DOI   ScienceOn
4 Pegg DE. The history and principles of cryopreservation. Semin Reprod Med 2002;20:5-13.   DOI   ScienceOn
5 Rall WF, Fahy GM. Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification. Nature 1985;313:573-5.   DOI   ScienceOn
6 Oh YH, Che ZM, Hong JC, Lee EJ, Lee SJ, Kim J. Cryopreservation of human teeth for future organization of a tooth bank: a preliminary study. Cryobiology 2005;51:322-9.   DOI   ScienceOn
7 Temmerman L, Dermaut LR, De Mil M, Van Maele G, Beele H, De Pauw GA. Influence of cryopreservation on human periodontal ligament cells in vitro. Cell Tissue Bank 2008;9:11-8.   DOI   ScienceOn
8 Kasai M, Mukaida T. Cryopreservation of animal and human embryos by vitrification. Reprod Biomed Online 2004;9:164-70.   DOI   ScienceOn
9 Takahashi T, Hirsh A, Erbe EF, Bross JB, Steere RL, Williams RJ. Vitrification of human monocytes. Cryobiology 1986;23:103-15.   DOI   ScienceOn
10 Decherchi P, Lammari-Barreault N, Cochard P, Carin M, Rega P, Pio J, et al. CNS axonal regeneration with peripheral nerve grafts cryopreserved by vitrification: cytological and functional aspects. Cryobiology 1997;34:214-39.   DOI   ScienceOn
11 Song YC, Khirabadi BS, Lightfoot F, Brockbank KG, Taylor MJ. Vitreous cryopreservation maintains the function of vascular grafts. Nat Biotechnol 2000;18:296-9.   DOI   ScienceOn
12 Pegg DE. The role of vitrification techniques of cryopreservation in reproductive medicine. Hum Fertil (Camb) 2005;8:231-9.   DOI   ScienceOn
13 Fahy GM, MacFarlane DR, Angell CA, Meryman HT. Vitrification as an approach to cryopreservation. Cryobiology 1984;21:407-26.   DOI   ScienceOn
14 Makarevich AV, Chrenek P, Olexikova L, Popelkova M, Turanova Z, Ostro A, et al. Post-thaw survival, cell death and actin cytoskeleton in gene-microinjected rabbit embryos after vitrification. Theriogenology 2008;70:675-81.   DOI   ScienceOn
15 Armitage WJ. Survival of corneal endothelium following exposure to a vitrification solution. Cryobiology 1989;26:318-27.   DOI   ScienceOn
16 Bourne WM, Nelson LR. Human corneal studies with a vitrification solution containing dimethyl sulfoxide, formamide, and 1,2-propanediol. Cryobiology 1994;31:522-30.   DOI   ScienceOn
17 Armitage WJ, Hall SC, Routledge C. Recovery of endothelial function after vitrification of cornea at -110 degrees C. Invest Ophthalmol Vis Sci 2002;43:2160-4.
18 Schwartz O, Andreasen JO, Greve T. Cryopreservation before replantation of mature teeth in monkeys. (II). Effect of preincubation, different freezing and equilibration rates and endodontic treatment upon periodontal healing. Int J Oral Surg 1985;14:350-61.   DOI
19 Kristerson L, Johansson LA, Kisch J, Stadler LE. Autotransplantation of third molars as treatment in advanced periodontal disease. J Clin Periodontol 1991;18:521-8.   DOI