Browse > Article
http://dx.doi.org/10.5051/jpis.2010.40.3.105

Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts  

Choi, Eun-Jeong (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Yim, Ju-Young (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Koo, Ki-Tae (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Seol, Yang-Jo (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Lee, Yong-Moo (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Ku, Young (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Rhyu, In-Chul (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Chung, Chong-Pyoung (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Kim, Tae-Il (Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.40, no.3, 2010 , pp. 105-110 More about this Journal
Abstract
Purpose: It has been reported that low-level semiconductor diode lasers could enhance the wound healing process. The periodontal ligament is crucial for maintaining the tooth and surrounding tissues in periodontal wound healing. While low-level semiconductor diode lasers have been used in low-level laser therapy, there have been few reports on their effects on periodontal ligament fibroblasts (PDLFs). We performed this study to investigate the biological effects of semiconductor diode lasers on human PDLFs. Methods: Human PDLFs were cultured and irradiated with a gallium-aluminum-arsenate (GaAlAs) semiconductor diode laser of which the wavelength was 810 nm. The power output was fixed at 500 mW in the continuous wave mode with various energy fluencies, which were 1.97, 3.94, and 5.91 $J/cm^2$. A culture of PDLFs without laser irradiation was regarded as a control. Then, cells were additionally incubated in 72 hours for MTS assay and an alkaline phosphatase (ALPase) activity test. At 48 hours post-laser irradiation, western blot analysis was performed to determine extracellular signal-regulated kinase (ERK) activity. ANOVA was used to assess the significance level of the differences among groups (P<0.05). Results: At all energy fluencies of laser irradiation, PDLFs proliferation gradually increased for 72 hours without any significant differences compared with the control over the entire period taken together. However, an increment of cell proliferation significantly greater than in the control occurred between 24 and 48 hours at laser irradiation settings of 1.97 and 3.94 $J/cm^2$ (P<0.05). The highest ALPase activity was found at 48 and 72 hours post-laser irradiation with 3.94 $J/cm^2$ energy fluency (P<0.05). The phosphorylated ERK level was more prominent at 3.94 $J/cm^2$ energy fluency than in the control. Conclusions: The present study demonstrated that the GaAlAs semiconductor diode laser promoted proliferation and differentiation of human PDLFs.
Keywords
Alkaline phosphatase; Extracellular signal-regulated kinases; Fibroblasts; Periodontal ligament; Semiconductor diode lasers;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kreisler M, Al Haj H, d'Hoedt B. Clinical efficacy of semi-conductor laser application as an adjunct to conventional scaling and root planing. Lasers Surg Med 2005;37:350-5.   DOI   ScienceOn
2 Herascu N, Velciu B, Calin M, Savastru D, Talianu C. Lowlevel laser therapy (LLLT) efficacy in post-operative wounds. Photomed Laser Surg 2005;23:70-3.   DOI   ScienceOn
3 Lopes-Martins RA, Albertini R, Martins PS, Bjordal JM, Faria Neto HC. Spontaneous effects of low-level laser therapy (650 nm) in acute inflammatory mouse pleurisy induced by carrageenan. Photomed Laser Surg 2005;23:377-81.   DOI   ScienceOn
4 Stein A, Benayahu D, Maltz L, Oron U. Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 2005;23:161-6.   DOI   ScienceOn
5 do Nascimento PM, Pinheiro AL, Salgado MA, Ramalho LM. A preliminary report on the effect of laser therapy on the healing of cutaneous surgical wounds as a consequence of an inversely proportional relationship between wave-length and intensity: histological study in rats. Photomed Laser Surg 2004;22:513-8.   DOI   ScienceOn
6 Midda M. Lasers in periodontics. Newsl Int Acad Periodontol 1991;1:2-3.
7 Midda M. Lasers in periodontics. Periodontal Clin Investig 1992;14:14-20.
8 Midda M. The use of lasers in periodontology. Curr Opin Dent 1992;2:104-8.
9 Ando Y, Aoki A, Watanabe H, Ishikawa I. Bactericidal effect of erbium YAG laser on periodontopathic bacteria. Lasers Surg Med 1996;19:190-200.   DOI   ScienceOn
10 Aoki A, Sasaki KM, Watanabe H, Ishikawa I. Lasers in nonsurgical periodontal therapy. Periodontol 2000 2004;36:59-97.   DOI   ScienceOn
11 Shimizu N, Yamaguchi M, Goseki T, Shibata Y, Takiguchi H, Iwasawa T, et al. Inhibition of prostaglandin E2 and interleukin 1-beta production by low-power laser irradiation in stretched human periodontal ligament cells. J Dent Res 1995;74:1382-8.   DOI   ScienceOn
12 Folwaczny M, Mehl A, Aggstaller H, Hickel R. Antimicrobial effects of 2.94 microm Er:YAG laser radiation on root surfaces: an in vitro study. J Clin Periodontol 2002;29:73-8.   DOI   ScienceOn
13 Moritz A, Schoop U, Goharkhay K, Schauer P, Doertbudak O, Wernisch J, et al. Treatment of periodontal pockets with a diode laser. Lasers Surg Med 1998;22:302-11.   DOI   ScienceOn
14 Correa F, Lopes Martins RA, Correa JC, Iversen VV, Joenson J, Bjordal JM. Low-level laser therapy (GaAs lambda = 904 nm) reduces inflammatory cell migration in mice with lipopolysaccharide-induced peritonitis. Photomed Laser Surg 2007;25:245-9.   DOI   ScienceOn
15 Kreisler M, Christoffers AB, Al-Haj H, Willershausen B, d'Hoedt B. Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibro-blasts. Lasers Surg Med 2002;30:365-9.   DOI   ScienceOn
16 Pereira AN, Eduardo Cde P, Matson E, Marques MM. Effect of low-power laser irradiation on cell growth and pro-collagen synthesis of cultured fibroblasts. Lasers Surg Med 2002;31:263-7.   DOI   ScienceOn
17 Giannopoulou C, Cimasoni G. Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent Res 1996;75:895-902.   DOI   ScienceOn
18 Gumbiner BM. Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell 1996;84:345-57.   DOI   ScienceOn
19 Kim TI, Jang JH, Lee YM, Rhyu IC, Chung CP, Han SB, et al. Biomimetic approach on human periodontal ligament cells using synthetic oligopeptides. J Periodontol 2004;75:925-32.   DOI
20 Ozawa Y, Shimizu N, Kariya G, Abiko Y. Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 1998;22:347-54.   DOI   ScienceOn
21 Pinfildi CE, Liebano RE, Hochman BS, Ferreira LM. Helium-neon laser in viability of random skin flap in rats. Lasers Surg Med 2005;37:74-7.   DOI   ScienceOn
22 Conlan MJ, Rapley JW, Cobb CM. Biostimulation of wound healing by low-energy laser irradiation: a review. J Clin Periodontol 1996;23:492-6.   DOI   ScienceOn
23 Mester E, Spiry T, Szende B, Tota JG. Effect of laser rays on wound healing. Am J Surg 1971;122:532-5.   DOI   ScienceOn
24 Reddy GK, Stehno-Bittel L, Enwemeka CS. Laser photostimulation accelerates wound healing in diabetic rats. Wound Repair Regen 2001;9:248-55.   DOI   ScienceOn
25 Isaka J, Ohazama A, Kobayashi M, Nagashima C, Takiguchi T, Kawasaki H, et al. Participation of periodontal ligament cells with regeneration of alveolar bone. J Periodontol 2001;72:314-23.   DOI   ScienceOn
26 Lekic P, McCulloch CA. Periodontal ligament cell population: the central role of fibroblasts in creating a unique tissue. Anat Rec 1996;245:327-41.   DOI   ScienceOn
27 Kreisler M, Christoffers AB, Willershausen B, d'Hoedt B. Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study. J Clin Periodontol 2003;30:353-8.   DOI   ScienceOn
28 Kreisler M, Meyer C, Stender E, Daublander M, Willershausen-Zonnchen B, d'Hoedt B. Effect of diode laser irradiation on the attachment rate of periodontal ligament cells: an in vitro study. J Periodontol 2001;72:1312-7.   DOI