Browse > Article

Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-$1{\beta}$ stimulation  

Lee, Sool-Heon (Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University)
Park, Ji-Il (Department of Dental hygiene, Gwangju Health College)
Kim, Young-Joon (Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University)
Publication Information
Journal of Periodontal and Implant Science / v.39, no.3, 2009 , pp. 359-365 More about this Journal
Abstract
Purpose: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell. Methods: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-$1{\beta}$ on MTA was evaluated by ELISA and RT-PCR. Results: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-$1{\beta}$ group and MTA+IL-$1{\beta}$ group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-$1{\beta}$ group and Glass+IL-$1{\beta}$ group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression. Conclusions: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.
Keywords
biocompatible materials; cytokines; ELISA; mineral trioxide aggregate;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Giannopoulou C, Kama JJ, Mombelli A. Effect of inflammation, smoking, and stress on gingival, crevicular fluid cytokine level. Journal of Clinical Periodontology 2003;30:145-153   DOI   ScienceOn
2 Lerner UH. Inflammation-induced bone remodeling in periodontal disease and the influence of post menopausal osteoporosis. J Dent Res 2006;85:596-607   DOI   PUBMED   ScienceOn
3 Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature 2003;423:337-342   DOI   ScienceOn
4 Dalrymple SA, Slattery R, Aud DM, Krishna M, Lucan LA, Murray R. Interleukin-6 is required for a protective immune response to systemic Escherichia coli infection. Infect Immun 1996;64:3231-3235   PUBMED
5 Zhu Q, Haglund R, Safavi KE, Spangberg LSW. Adhesion of human osteoblasts on root-end filling materials. J Endod 2000;26:404-406   DOI   ScienceOn
6 Koh ET, McDonald F, Pitt Ford TR, Torabinejad M. Cellular response to Mineral Trioxide Aggregate. J Endod 1998;24:543-547   DOI   ScienceOn
7 Yavuzyilmaz E, Yamalik N, Bulut S, Ozen S, Ersoy F, Saatci U. The gingival crevicular fluid interleukin-1beta and tumor necrosis factor-a levels in patients with rapidly progressive periodontitis. Aust Dent J 1995;40:46-49   DOI   ScienceOn
8 Tsai CC, Hou YP, Chen CC. Levels of interleukin-1beta and interleukin-8 in gingival crevicular fluid in adult periodontitis. J Periodontol 1995;66:852-859   DOI   PUBMED   ScienceOn
9 Cory AH, Barltrop JA, Cory JG. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun 1991;3:207-212   PUBMED
10 Ishihara Y, Kuroyagani T, Shirozi N, Yamagishi E, Ohguchi M, Koide M, Ueda N, Amano K, Noguchi T. Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites. J Periodont Res 1997;32:524-529   DOI   ScienceOn
11 Torabinejad M, Hong CU, Pitt Ford TR, Kettering JD. Cytotoxicity of four root-end filling materials. J Endod 1995;21:489-492   DOI   ScienceOn
12 Pelliccioni GA, Ciapetti G, Cenni E, Granchi D, Nanni M, Pagani S, Giunti A. Evaluation of osteoblast-like cell response to ProRoot MTA cement. J Mat Sci 2004;15:167-173
13 Robert HW, Toth JM, Berzins DW, Charlton DG. Mineral trioxide aggregate material use in endodontic treatment: a review of the literature. Dent Mater 2008;24:149-164   DOI   ScienceOn
14 Nakashima T, Kobayashi Y, Yamasaki S, Kawakami A, Eguchi K, Sasaki H, Sakai H. Protein expression and functional difference of membrane-bound and soluble receptor activator of NF-kappaB ligand : Modulation of the expression by osteotropic factors and cytokines. Biochem Biophys Res Commun 2000;275:768-775   DOI   ScienceOn
15 Barrieshi-Nusair K, Qudeimat MA. A prospective clinical study of mineral trioxide aggregate for partial pulpotomy in cariously exposed permanent teeth. J Endod 2006;32:731-735   DOI   ScienceOn
16 Ishihara K, Hirano T. IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytoline Growth Factor Rev 2002;13:357-368   DOI   ScienceOn
17 Torabinejad M, Hong CU, McDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod 1995;21:349-353   DOI   ScienceOn
18 Cochran DL. Inflammation and bone loss in periodontal disease. J Periodontol. 2008;79(Suppl):1569-1576   DOI   PUBMED   ScienceOn
19 Kobayashi N, Kadono Y, Naito A, Matsumoto K, Yamamoto T, Tanaka S, Inoue J. Segregation of TRAF6-mediated signaling pathways clarifies its role in osteoclastogenesis. EMBO J 2001;20:1271-1280   DOI   ScienceOn
20 Nakayama A, Ogiso B, Tanabe N, Takeichi O, Matsuzaka K, Inoue T. Behaviour of bone marrow osteoblasts-like cells on mineral trioxide aggregate: morphology and expression of type 1 collagen and bone-related protein mRNAs. Int Endodont J 2005;38:203-210   DOI   ScienceOn
21 Siggelkow H, Eidner T, Lehmann G, Viereck V, Raddatz D, Munzel U, Hein G, Hufner M. Cytokine, osteoprotegerin, and RANKL in vitro and histomorphometric indices of bone turnover in patients with different bone disease. J Bone Miner Res 2003;18:529-538   DOI   ScienceOn
22 Hofbaur LC, Heufelder AE. Role of receptor activator of nuclear factor-kB ligand and osteoprotegerin in bone cell biology. J Mol Med 2001;79:243-253   DOI   ScienceOn