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Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects  

Kwon, Suk-Hoon (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Lim, Hyun-Chang (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Choi, Kyung-Hee (Research Development Institute, Cowellmedi Co. LTD)
Kim, Min-Soo (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Lee, Ji-Hyun (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Jung, Ui-Won (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Yun, Jeong-Ho (Department of Dentistry, College of Medicine, Kwandong University, Myongji Hospital)
Kim, Chang-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Cho, Kyoo-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
Publication Information
Journal of Periodontal and Implant Science / v.39, no.1, 2009 , pp. 77-86 More about this Journal
Abstract
Purpose: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system. Materials and methods: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals. Results: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks. Conclusion: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.
Keywords
bone morphogenetic protein-2; Ecoli expression system; Absorbable collagen sponge; rat calvarial defect;
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