Browse > Article

Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide  

Kim, Jung-Eun (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Lee, Sung-Hoon (Oral Infection and Immunity, School of Dentistry, Seoul National University)
Choi, Bong-Kyu (Oral Infection and Immunity, School of Dentistry, Seoul National University)
Koo, Ki-Tae (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Kim, Tae-Il (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Lee, Yong-Moo (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Ku, Young (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Chung, Chong-Pyoung (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Rhyu, In-Chul (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
Publication Information
Journal of Periodontal and Implant Science / v.38, no.3, 2008 , pp. 543-550 More about this Journal
Abstract
Purpose: The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. Material and Methods: After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria. Result: The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-$1{\beta}$ was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs. Conclusion: The membrane protein of T. forsythia could be one of virulence factors.
Keywords
Tannerella forsythia; virulence factors; human gingival fibroblasts;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Loomer PM. Microbiological diagnostic testing in the treatment of periodontal diseases. Periodontol 2000 2004;34:49-56   DOI   ScienceOn
2 Yang HW, Huang YF, Chou MY. Occurrence of Porphyromonas gingivalis and Tannerella forsythensis in periodontally diseased and healthy subjects. J Periodontol 2004;75:1077-1083   DOI   ScienceOn
3 Onishi S, Honma K, Liang S, et al. Toll-like receptor 2-mediated interleukin-8 expression in gingival epithelial cells by the Tannerella forsythia leucine-rich repeat protein BspA. Infect Immun 2008;76:198-205   DOI   ScienceOn
4 Sharma A, Inagaki S, Sigurdson W, Kuramitsu HK. Synergy between Tannerella forsythia and Fusobacterium nucleatum in biofilm formation. Oral Microbiol Immunol 2005;20:39-42   DOI   ScienceOn
5 Ikegami A, Honma K, Sharma A, Kuramitsu HK. Multiple functions of the leucine-rich repeat protein LrrA of Treponema denticola. Infect Immun 2004;72:4619-4627   DOI   ScienceOn
6 Nishihara T, Koseki T. Microbial etiology of periodontitis. Periodontol 2000 2004;36:14-26
7 Tanner AC, Haffer C, Bratthall GT, Visconti RA, Socransky SS. A study of the bacteria associated with advancing periodontitis in man. J Clin Periodontol 1979;6: 278-307   DOI
8 Hasebe A, Yoshimura A, Into T, et al. Biological activities of Bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease. Infect Immun 2004;72:1318-1325   DOI
9 Socransky SS, Haffajee AD. Dental biofilms: difficult therapeutic targets. Periodontol 2000 2002;28:12-55   DOI   ScienceOn
10 Welin J, Wilkins JC, Beighton D, Svensater G. Protein expression by Streptococcus mutans during initial stage of biofilm formation. Appl Environ Microbiol 2004;70:3736-3741   DOI   ScienceOn
11 Takaishi Y, Morii H, Miki T. The benzoyl-DL arginine-naphthylamide (BANA) test and polymerase chain reaction measurement of pathogenic bacteria can assess the severity of periodontal disease. Int J Tissue React 2003;25: 19-24   PUBMED
12 Timmerman MF, van der Weijden GA. Risk factors for periodontitis. Int J Dent Hyg 2006;4:2-7   DOI   PUBMED   ScienceOn
13 Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL, Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25:134-144   DOI   ScienceOn
14 Kapatral V, Anderson I, Ivanova N, et al. Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586. J Bacteriol 2002;184: 2005-2018   DOI
15 Bodet C, Chandad F, Grenier D. Inflammatory responses of a macrophage/epithelial cell co-culture model to mono and mixed infections with Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. Microbes Infect 2006;8: 27-35   DOI   ScienceOn
16 Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontol 2000 2005;38:135-187   DOI   ScienceOn
17 Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet 2005;366:1809-1820   DOI   ScienceOn
18 Tanner A, Maiden MF, Macuch PJ, Murray LL, Kent RL, Jr. Microbiota of health, gingivitis, and initial periodontitis. J Clin Periodontol 1998;25:85-98   DOI   ScienceOn
19 Seshadri R, Myers GS, Tettelin H, et al. Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes. Proc Natl Acad Sci U S A 2004;101:5646-5651   DOI   ScienceOn
20 Nelson KE, Fleischmann RD, DeBoy RT, et al. Complete genome sequence of the oral pathogenic Bacterium Porphyromonas gingivalis strain W83. J Bacteriol 2003;185: 5591-5601   DOI
21 Hajishengallis G, Martin M, Sojar HT, et al. Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol 2002;9:403-411   PUBMED
22 Tanner AC, Socransky SS, Goodson JM. Microbiota of periodontal pockets losing crestal alveolar bone. J Periodontal Res 1984;19:279-291   DOI
23 Into T, Nodasaka Y, Hasebe A, et al. Mycoplasmal lipoproteins induce toll-like receptor 2- and caspases-mediated cell death in lymphocytes and monocytes. Microbiol Immunol 2002;46:265-276   DOI   PUBMED