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Autotransplantation using the acellular dermal matrix seeded by periodontal ligament fibroblasts in minipig: histological evaluation as potential periodontal ligament substitutes  

Yu, Sang-Joun (Department of Periodontology, College of Dentistry, Chosun University)
Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute)
Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University, Oral Biology Research Institute)
Jang, Hyun-Seon (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute)
Publication Information
Journal of Periodontal and Implant Science / v.37, no.1, 2007 , pp. 53-64 More about this Journal
Abstract
The aim of this study was to examine the possibility of periodontal ligament regeneration when autotransplantation was used by the periodontal ligament fibroblasts cultured on the acellular dermal matrix in teeth without a periodontal ligament. One minipig was used in this study. The mandibular and maxillary permanent incisors were ex-tracted for the culture of the periodontal ligament cells. The roots of the unextracted teeth were classified into a positive control group, in which the normal periodontal ligament was preserved. The roots of the extracted teeth were divided into the following two groups: The negative control group, in which the periodontal ligament had been removed and the acellular dermal matrix was not applied; and an experimental group, in which the periodontal ligament had been removed and periodontal ligament fibroblast cultured on an acellular dermal matrix was applied. The prepared teeth were transplanted, and completely submerged using physical barrier membranes. The animal was sacrificed 4 weeks after the autotransplant. The transplanted teeth were examined histologically. In this study, the periodontal ligament was normal in the positive control group, and ankylosis was discovered on the denuded root surface in the negative control group. Periodontal ligament-like connective tissue was found adjacent to the denuded root and the new cementum-like layer of hard tissue was formed in the experimental group. These results suggest that the periodontal ligament fibroblasts cultured on the acellular dermal matrix may play a role in regenerating the periodontal ligament-like tissue with new cememtum-like tissue formation.
Keywords
autotransplantation; periodontal ligament; cell-seeding; acellular dermal matrix;
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1 Andreasen JO, Hjorting-Hansen E. Replantation of teeth. II. Hisgtological study of 22 replanted anterior teeth in humans. Acta Odont Scand 1966;24:287-306   DOI
2 Andersson L, Jonsson BG, Hammarstrom L. Evaluation of statistics and desirable experimental design of a histomorphometrical method for studies of root resorption. Endod Dent Traumatol 1987;3:288-295   DOI
3 Andreasen JO. External root resorption: its implication in dental traumatology, paedodontics, periodontics, orthodontics and endodontics. Int Endod J 1985;18:109-118   DOI
4 Andreasen JO. Tooth Reimplantation and transplantation, Paris: Masson; 1993: 130
5 Andreasen JO. Tooth Reimplantation and transplantation. Paris: Masson; 1993: 199
6 ElDeeb ME, Andreasen JO. Histometric study on the effect of occlusal alteration on periodontal tissue healing after surgical injury. Endod Dent Traumatol 1991:7:158-163   DOI
7 Wesselink PR, Beertsen W. Initiating factors in dental root resroption, In:Davidotitch Z, ed, Biological mechanisms of tooth eruption and root resorption. Birmingham: EB-SCO Media, 1988:329-334
8 Wesselink PR, Beertsen W. Repair processes in the periodontium following dento-alveolar ankylosis: the effect of masticaatory function. J Clin Periodontol 1994;21: 472-478   DOI   ScienceOn
9 Beertsen W, McCulloch CA, Sodek J. The periodontal ligament: a unique, multifunctional connective tissue. Periodontolgy 2000 1997; 13: 20-40   DOI   ScienceOn
10 Andreasen JO. Evaluation of different types of autotransplanted connective tissues as potential periodontal ligament substitutes. Int J Oral Surg 1981:10:189-201   DOI
11 Plagmann HC, Lange DE, Bernimoulin JP, Howe H. Experimentelle Studie uberdie Epitelneubildung bei heterotopischen Bindegewebstansplantaten. Dtsch, Zahnaertztl. Z. 1974:29:497-503
12 Jun Isaka, Atsuchi O, Makoto K. Participation of periodontal ligament cells with regeneration of alveolar bone. J Periodontol 2001;72:314-323   DOI   ScienceOn
13 Akizuki T, Oda S, Komaki M, Tsuchioka H, Kawakatsu N, Kikuchi A, Yamato M, Okano T, Ishikawa I, Application of periodontal ligament cell sheet for periodontal regeneration : a pilot study in beagle dogs. J Periodont Res 2005;40:245-251   DOI   ScienceOn
14 김성선, 김병옥, 박주철, 장현선, Scaffold상에 식립한 사람치주인대섬유모세포를 통한 치주조직공학. 대한치주과학회지 2006;36:757-766   과학기술학회마을
15 Arceo N, Sauuk JJ, Moehring J, Foster RA, Somerman MJ. Human periodontal cells initiate mineral-like nodules in vitro, J Periodontol 1991;62:499-503   DOI   PUBMED
16 Gottlow J, Nyman S, Karring T, Lindhe J. New attachment formation as the result of contolled tissue regeneration. J Clin Periodontol 1984;11:494-503   DOI
17 Mukai M, Yoshimine Y, Akamine A, Maeda K. Bone-like nodules formed in vitro by rat periodontal ligament cells. Cell Tissue Res 1993;271:453-460   DOI
18 Aukhil I, Pettersson E, Suggs C, Periodontal wound healing in the absence of periodontal ligament cells. J Periodontol 1987;58:71-77   DOI   PUBMED
19 Magnusson I, Nyman S, Karring T, Egelberg J. Connective tissue attachment formation following exclusion of gingival connective tissue and epithelium during healing. J Periodont Res 1985;20:201-208   DOI
20 Caffesse R, Smith B, Duff B, Mporrison EC, Becker W. Class II furcations treated by guided tissue regeneration in humans: Case reports. J Periodontol 1990;61:510-514   DOI   PUBMED
21 Mark Bartold P, Christopher A, McCulloch G, Sampath A Tissue engineerting: a new paradigm for periodontal regeneration based on molecular and cell biology. Periodontology 2000 2000;24:253-269
22 Ogiso B, Hughes JI, Melcher AH, McCulloch CAG. Fibroblasts inhibt mineralized bone nodule formation by rat bone marrow stro mal cells in vitro. J Cell Physiol 1991;146: 442-450   DOI
23 Lang H, Sch ler N, Arnhold S, Nolden R, Mertens T. Formation of differentiated tissues in vivo by periodontal cell populations cultured in vitro. J Dent Res 1995;74:1219-1225   DOI   ScienceOn
24 Lekic PC, Rahshankar D, Chen H, Tenenbaum H, McCulloch CAG. Transplantation of labeled periodontal ligament cells promotes regeneration of alveolar bone. Anat Rec 2001;262:193-202   DOI   ScienceOn
25 Gottlow J, Karring T, Nyman S. Guided tissue regeneration following treatment of recession-type defects in the monkey. J Periodontol 1990;61:680-685   DOI   PUBMED
26 Andreasen JO, Hjorting-Hansen E. Replantation of teeth I. Radiographic and clinical study of 110 human teeth replanted after accidental loss. Acta Odont Scand 1966;24:263-286   DOI
27 Akbay A, Baran C, Gunhan O, Ozmeric N, Balos K. Periodontal regenerative potential of autogenous periodontal ligament grafts in Class II furcation defects. J Periodontol 2005;76:595-604   DOI   ScienceOn
28 Donath K, Breuner G. A method for the study of undecalcified bones and teeth with attached soft tissues. The sawing and grinding technique. J Oral Pathol 1982;11: 318-326   DOI
29 Lang H, Sch ler N, Nolden R. Attachment formation following replantation of cultured cells into periodontal defects-a study in minipigs. J Dent Res 1998;77:393-405   DOI   ScienceOn
30 Lindskog S, Bloml f I, Hammarstr m L. Evidence for a role of odontogenic epithelium in maintaining the periodontal space. J Clin Periodontol 1988;15:371-373   DOI
31 Andreasen JO, Tooth Reimplantation and transplantation. Paris: Masson; 1993: 169
32 Bernimoulin JP, Nalbandian J, LoeH. Effect of dermal connective tissue on cemental healing in the dog. J Dent Res 1978: 57: Abstr, 99
33 Andreasen JO. Experimental dental traumatology: development of a model for external root resorption. Endod Dent Traumatol 1987;3:269-287   DOI
34 Andreasen JO. Relationship between cell damage in the periodontal ligament after replantation and subsequent development of root resorption. A time-related study in monkeys. Acta Odont Scand 1981:39:15-25   DOI   ScienceOn
35 Van Dijk LJ, Schakenraad JM, Van der Voort HM, Busscher HJ. Cell-seeding of periodontal ligament fibroblasts A novel technique to created new attachment A pilot study. J Clin Periodontol 1991;18:196-199   DOI
36 Levesey SA, Herndon DN, Hollyoak MA, Atkinson YR, Nag A Transplanted acellular allograft dermal matrix. Potential as a template for the reconstruction of viable dermis. Transplantation 1995;60:1-9   DOI
37 Selvig K, Nilveus RE, Fitzmorris L, Kersten B, Khorsandi SS. Scanning elecmicroscopic observations of cell population and bacterial contamination of membranes used for guided periodontal tissue regeneration in humans. J Periodontol 1990; 61:515-520   DOI   PUBMED
38 Nyman S, Gottlow J, Karring T, Lindhe J. The regenerative potential of the periodontal ligament. An experiomental study in the monkey. J Clin Periodontol 1982;9:157-265
39 Shulman J. Clinical evaluation of an acellular dermal allograft for increasing the zone of attached gingiva. Pract Periodontics Aesthet Dent 1996;8:201-208   PUBMED
40 Nyman S, Karring T, Lindhe J, Planten S. Healing following implantation of periodontitis-affected roots into gingival connective tissue. J Clin Periodontol 1980;7:394-401   DOI
41 Lang H, Schuler N, Nolden R. Attachment formation following replantation of cultured cells into periodontal defects - a study in minipigs, J Dent Res 1998;77: 393-405   DOI   ScienceOn
42 Villanueva AR. A bone stain for osteoid seams in fresh, unembedded, mineralized bone. Stain Technol 1974;49:1-8   DOI   PUBMED
43 Haney JM, Nilv us RE, McMillan PJ, Sikesj UME. Periodontal repair in dogs: Expanded polytetrafluoroethylene barrier membranes support wound stabilization and enhance bone regeneration. J Periodontol 1993; 64: 883-890   DOI   PUBMED   ScienceOn
44 Toriumi DM, East CA, Larrabee WF. Osteoinductive biomaterials for medical implantation. J Long Term Effects Med Implants 1991;1:53-77
45 Andreasen JO. The effect of extraalveolar period and storage media upon periodontal and pulpal healing after replantation of mature permanent incisors in monkeys. Int J Oral Surg 1981;10:43-53   DOI
46 Anderson L, Lindskog S, Blomlof L, Hedstrom KG. Effect of masticatory stimulation on dento-alveolar ankylosis after experimental tooth replantation. Endod Dent Traumatol 1985;1:13-16   DOI
47 Gottlow J, Nyman S, Lindge J, Kariing T, Wennstrom J. New attachment formation in the human periodontium by guided tissue regeneration. Case reports. J Clin Periodontol 1986;13:604-616   DOI
48 Caffesse R, Smith B, Castelli W, Nasjleti C. New attachment achieved by guided tissue regeneration in beagle dogs. J Periodontol 1988;59:589-594   DOI   PUBMED
49 Gogolewski S, Jovanovic M, Perren SM, Dillon JG, Hughes MK. Tissue response and in vivo degradation of selected polyhydroxyacids: polyactides (PLA) , poly (3-hydroxybutyrate) (PHB) and poly (3-hydroxybutyrate-co-3- hydroxyvalerate) (PHB/VA). J Biomed Mater Res 1993;27: 1135-1148   DOI   ScienceOn
50 Marmary Y, Brunette DM, Heersche JNM. Differences in vitro between cells derived from periodontal ligament and skin of Macaca irus, Arch Oral Biol 1976;21:709-716   DOI   ScienceOn
51 Phillippe C, Gault, Rita WC. Tooth auto-transplantation with double periodontal ligament stimulation to replace periodontally compromised teeth. J Periodontol 2002:73:575-583   DOI   ScienceOn
52 Andreasen, JO. The effect of pulp extirpation or root canal treatment upon periodontal healing after replantation of permanent incisors in monkeys, J Endodontol 1981:7:245-252   DOI   ScienceOn
53 Andreasen JO, Kristerson L. The effect of limited drying or removal of the periodontal ligament. Periodontal healing after replantation of mature permanent incisors in monkeys. Acta Odontol Scand 1981:39:1-13   DOI   PUBMED   ScienceOn
54 Loe H and Waerhaug J. Experimental replantation of teeth in dogs and monkeys. Arch Oral Biol 1961;3:176-184   DOI   ScienceOn
55 Andreasen JO. Relationship between surface and inflammatory resorption and changes in the pulp after replantation of permanent incisors in monkeys. J Endod 1981; 7: 294-301   DOI   ScienceOn