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The Regenerative effects of Platelet-Rich Plasma and Enamel Matrix Protein on Grade III Furcation defects in beagle dogs  

Kim, Young-Jun (Department of Periodontology, College of Dentistry, Dan-Kook University)
Lim, Sung-Bin (Department of Periodontology, College of Dentistry, Dan-Kook University)
Chung, Chin-Hyung (Department of Periodontology, College of Dentistry, Dan-Kook University)
Hong, Ki-Seok (Department of Periodontology, College of Dentistry, Dan-Kook University)
Publication Information
Journal of Periodontal and Implant Science / v.35, no.4, 2005 , pp. 823-837 More about this Journal
Abstract
The purpose of this study was to study the histopathological correlation between the use of platelet-rich plasma and enamel matrix protein used in conjunction with xenograft. compared to a control group with regards to bone regeneration at the grade III furcation area in beagle dogs. Control group was treated with bovine derived bone $powder(Biocera^{(R)})$, and experimental I group was treated with bovine derived bone powder and Platelet-rich plasma and experimental II group was treated with bovine derived bone powder and Enamel matrix $protein(Emdogain^{(R)})$. The regeneration rate of bone formation was observed and compared histopathologically at 2. 4, and 8 weeks after surgery. The results were as follows: 1. In control group and both experimental groups. inflammatory cells were observed but, new bone formation wasn't. 2. In control group, new cementum on the notch was found in 4 weeks, less mature periodontal ligament when compared to that of experimental group was found and cementum formation was great but, regeneration couldn't be seen in 8 weeks. 3. Experimental I group. new bone formation in the area adjacent to alveolar bone and graft material surrounded by more dense connective tissue were found in 4 weeks. New bone formation up to crown portion was found and periodontal ligament was aligned functionally and cementum more mature. 4. Experimental II group, new bone formation was found under the defect area in 4 weeks and new bone formation around graft material in 8 weeks, too, and there were a number of fibroblasts, blood vessels, acellular cementum, which was less mature when compared to that of experimental I group, and dense collagen fiber like which normal periodontal ligament has in periodontal ligament of experimental II group in 8 weeks. 5. As a result of histologic finding, bone formation rate were 18.0${\pm}$7.87%(control group), 34. 05${pm}$7.25%(experimental I group), 19.33 ${pm}$5.15%(experimental II group) in 4 weeks and 21.89${pm}$1.58%(control group), 38.82${pm}$3.2(experimental I group), 37.65${pm}$9.22%(experimental II group) in 8 weeks. 6. Statistically significant ratio of bone formation was observed in experimental I group in 4 weeks and in experimental II group in 8 weeks. When experimental I group was compared to experimental II group, the ratio of bone formation in experimental I group was higher than that in experimental II group in 4 weeks(p<0.05). This results suggest that platelet-rich plasma showed more new bone formation than enamel matrix protein within 4 weeks. And use of enamel matrix protein in the treatment of periodontal bone defects starts to enhance regeneration after 8 weeks in beagle dogs.
Keywords
platelet-rich plasma; enamel matrix protein; regeneration;
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1 Matsuda N, Lin WL, Kumar NM, Cho MI and Genco RJ.: Mitogenic chemotactic and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro. J Periodontol 1992; 63: 515-525   DOI   PUBMED
2 Subburaman Mohan: Bone growth Factors. Clinical Orthopaedics and related research 1991:263:30-48   PUBMED
3 Tayapongsak P, O'Brien DA, Monteiro CB, Arceo-Diaz LL,: Autologous fibrin adhesive in mandibular reconstruction with particulate cancellous bone and marrow. J Oral Maxillofac Surg 1994:52 :161-166   DOI   ScienceOn
4 Machtei. E. E., Cho, M. I., Dunford, R, Norderyd, J., Zambon, J. J., Genco, R. J. : Clinical, microbiological, and histological factors which influence the success of regenerative periodontal therapy. J Periodontol 1994:65:154-161   DOI   PUBMED   ScienceOn
5 Sculean A, Chiantella GC, Windisch P, Gera I, Reich E : Clinical evaluation of an enamel matrix protein derivative (Emdogain) combined with a bovinederived xenograft (Bio-Oss) for the treatment of intrabony periodontal defects in humans. Int J Periodontics Restorative Dent 2002:22:259-267   PUBMED
6 Velasquez-Plata D, Scheyer ET, Mellonig JT. Clinical comparison of an enamel matrix derivative used alone or in combination with a bovine-derived xenograft for the treatment of periodontal osseous defects in humans. J Periodontol 2002:73:433-440   DOI   ScienceOn
7 MG. Newman, HH Takei, FA Carranza: Clinical Periodontology, ed 9. Philadelphia, W. B. Sauders Co, 2002
8 Marx RE. Carlson ER, Eichstaedt RH, Schimmele SR, Strauss JE. Georgeff KR: Platelet-rich plasma : Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol 1998:85:638-646   DOI   ScienceOn
9 Lynch SE, Williams RC, Polson AM, Howell TH, Reddy Ms. Zappa UE, Antoniades HN. : A combination of plateletderived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 1989: 16:545-548   DOI   PUBMED
10 Postlethwaite AE. Jorma KO, Moses HL. Kang AH. : Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor. J Exp Med 1987:65:251-256
11 Martu S, Burlui V, Mocanu C, Forna N : Periodontal regeneration with enamel derivate proteins (Emdogain)-clinical evaluation. Rev Med Chir Soc Med Nat lasi 2000: 104: 147-151
12 Meffert, R. M., Thomas, J. R : Hydroxylapatite as an alloplastic graft in the treatment of human periodontal osseous defects. J Periodontol 1985;56:63-73   PUBMED
13 Isidor, F., Karring, T. : New attachment formation on citric acid treated roots. J Periodont. Res. 1985; 20:421-430   DOI   PUBMED
14 Nilveus, R. egelberg, J. : The effect of topical citrical acid application of the healing of experimental furcation defects in dogs. The relative importance of coagulum support, flap design and systemic antibiotics. J Periodont. Res. 1980 ; 15: 551-560   DOI   PUBMED
15 Lynch SE. : Platelet-derived growth factor and insulin-like growth factor. I. Mediators of healing in soft tissue and bone wounds. Periodont Case Rep 1991: 13: 13-20
16 Rutherford, R. B., Hiekrash, C.E. : Platelet-derived growth factor and insulin-like growth factors stimulate regeneration of periodontal attachment in monkeys. J Periodont. Res. 1992:27:285 -290   DOI   PUBMED
17 Souyris. F. Coral, a new biomedicals materials. J. Max-fac. Surg 1985: 13:64
18 Terranova VP, Franzetti LC, Hick S, Wikesjö VME. : Biochemically mediated periodontal regeneration. J Periodont Res 1987;22:248-251   DOI   PUBMED
19 치주과학교수협의회: 치주과학(The text book of periodontology), 4판, 군자출판사 2004
20 Canalis E, Mc Carthy TL, Centrella M. : The role of growth factors in skeletal remodelling. Endocrimol Meta Clin North Am 1989;18:903-912
21 Venezia E. Goldstein M. Schwartz Z : The use of enamel matrix derivative in periodontal therapy. Refuat Hapeh Vehashinayim 2002: 19: 19-34, 88   PUBMED
22 James T. Mellonig : Enamel matrix derivative for periodontal reconstructive surgery: Technique and clinical and histologic case report. Int J Periodontics Restorative Dent 1999: 19: 9-19
23 Pontoriero, R, Nyman, S., Lindhe, J. : Guided tissue regeneration in surgically produced furcation defects. An experimental study in the beagle dogs. J Periodontol 1992: 19: 159-163   DOI   PUBMED
24 Howell TH, Fiorellini JP, Paquette DW. : A phase I/II clinica trial to evaluation a combination of recombinant human platelet derived growth factor-BB and recombinant human insulin-like growth factor-I in patients with periodontal disease. J Periodontol 1997: 68: 1186-1193   DOI   PUBMED   ScienceOn
25 Scheyer ET, Velasquez-Plata D, Brunsvold MA, Lasho DJ, Mellonig JT. A clinical comparison of a bovine-derived xenograft used alone and in combination with enamel matrix derivative for the treatment of periodontal osseous defects in humans. J Periodontol 2002:73:423-432   DOI   ScienceOn
26 Raymond A. Yukna and James T. Mellonig : Histologic evaluation of periodontal healing in humans following regenerative therapy with enamel matrix derivative. A 10-case series. J Periodontol 2000:71(5) :752-759   DOI   ScienceOn
27 Rasperini G, Ricci G, Silvestri M : Surgical technique for treatment of infrabony defects with enamel matrix derivative(Emdogain): 3 case reports. Int J Periodontics Restorative Dent 1999; 19: 578-587   PUBMED
28 Terranova VP, Hick S, Franzetti L, Lyall RM, Wikesso VME. : A biochemical approach to periodontaol regeneration, AFS CM, Assay for specific cell migration. J Periodontol 1987;58:247-259   DOI   PUBMED
29 임성빈, 이광수, 박영채, 유형근, 신형식. 성견 2급 이개부 병변 치료 시 이종골 이식 및 혈소판 농축 혈장의 골 재생에 관한 연구. 대한치주과학회지 2000;30:257-275
30 Keski-Oja J, Leof EB, Lyons RM. Coffey RJ, Jr. Moses HL.: Transforming growth factor and control of neoplastic cell growth. J Cell Biochem 1987: 33: 95-107   DOI   PUBMED
31 Antosz ME, Bellows CG, Aubin JE.: Effects of TGF-$\beta$ and epidermal growth factor on celll proliferation and the formation of bone nodules in isolated fetal rat calvaria cells. J Cell Physiol 1989: 140: 386-393   DOI   PUBMED
32 Hammarstrom L. Enamel matrix, cementum development and regeneration. J Clin Periodontol 1997:24:658-668   DOI   ScienceOn
33 Lekovic, V, Kenney, E. B., Carranza, F. A., Martignoni, M. : The use of autogenous periosteal grafts as barriers of the treatment of class II furcation involvements in lower molars. J Periodontol 1991: 62: 775-780   DOI   PUBMED
34 Rutherford RB, Trilsmith MD, Ryan HE and Charette MF.: Synergistic effects of dexamethasone on platelet-derived growth factors mitogenesis in vitro. Arch Oral Biol 1992;37:139-145   DOI   ScienceOn
35 Antoniades HN, Scher CD, Stiles CD. : Purification of human platelet-derived growth factor. Cell Biol 1979;76:1809-1813
36 Giannobile WV, Finkelman RD, Lynch SE : Comparison of canine and non- human primate animal models for periodontal regenerative therapy: Results following a single administration of PDGF/ IGF-I. J Periodontol 1994; 65: 1158-1168   DOI   PUBMED   ScienceOn
37 Turner. D.W. and Melloonig.J.T : Antigenicity of freeze-dried bone allograft in periodontal osseous defects. J. periodontal Res 1981: 16:89-99   DOI   PUBMED
38 A.M. Hoang. R.J. Klebe. B. Steffensen, O.H. Ryu, J.P. Simmen, and D.L. Cochran : Amelogenin is a cell adhesion protein. J Dent Res 2002; 81 : 497-500   DOI   ScienceOn
39 Hideaki Hirooka. The biologic concept for the use of enamel matrix protein: True periodontal regeneration. Quintessence international 1998:29:621-630
40 Terranova VP, Franzetti LC, Hick S, Wikesiö VME. : Biochemically mediated periodontal regeneration. J Periodont Res 1987:22:248-251   DOI   PUBMED
41 Caffesse, R. G., Smith, B. A. : New attachment achieved by guided tissue regeneration in the beagle dogs. J Periodontol 1988:59:589-594   DOI   PUBMED
42 Kazuhiro Okuda, Manabu Momose. Akira Miyazaki, Masashi Murata, Shigeru Yokoyama, Yukari Yonezawa, Larry F. Wolff. Hiromasa Yoshie. Enamel Matrix Derivative in the treatment of human intrabony osseous defects. J Periodontol 2000:71:1821-1828   DOI   ScienceOn
43 Terranova VP, Hick S, Franzetti L, Lyall RM. Wikesjö VME. : A biochemical approach to periodontaol regeneration, Assay for specific cell migration. J Periodontol 1987; 58:247-259   DOI   PUBMED
44 Sporn ME, Roberts AB, Wakefield LM, de Crombrugghe B.: Some recent advances in the chemistry and biology of TGF-$\beta$. J Cell Biol 1987:105:1039-1045   DOI   ScienceOn
45 Oates TW, Rouse CA, Cochra DI : Mitogenic effects of growth factors on human periodontal ligament cells in vitro. J Periodontol 1993;64: 142-148   DOI   PUBMED   ScienceOn
46 Moon-II Cho, Wen-Lang Lin : Platelet-Derived Growth Factor-Modulated Guided Tissue Regenerative Therapy. J Periodontol 1995: 66: 522-530   DOI   ScienceOn
47 Pfeilschifter J, Oechsner M. Naumann A, Gronwald RGK, Minne HW, Ziegler R : Stimulation of bone matrix apposition in vitro by local growth factors : A comparison between insulin-like growth factor I, platelet-derived growth factor, and transforming growth factor. Endocrinology 1990: 127:69-75   DOI   ScienceOn
48 Lynch SE, Colvin RB, Antoniades HN: Growth factors in wound healing' Single and synergistic effects on partial thickness porcine skin wounds. J Clin Invest 1989:84:640-646   DOI
49 Brooks. D.B. Heiple.K.G. et al : Immunological factors in homogeneous bone transplantation. IV. The effect of various methods of preparation and irradiation on antigenicity. J. bone Joint Surg 1963 :45: 1617   DOI
50 Stahl, S. Frou, S. : Histologic and clinical responses to porous hydroxylapatite implants in human periodontal defects. J Periodontol 1987: 58: 689-695   DOI   PUBMED
51 Metzler, D. G., Seamons, B. C., Mellonig. J. T., Gher, M. E., Gray, J. L. : Clinical evaluation of guided tissue regeneration in the treatmnet of maxillary class II molar furcation invasions. J Periodontol 1993: 62: 353-360
52 Centrella M, McCarthy TL, Canalis E. : TGF-$\beta$ is a bifunctional regulator of recplication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone. J Bio Chem 1987:262: 2874-2896
53 Donos N, Sculean A. Glavind L, Reich E, Karring T : Wound healing of degree III fur cation involvements following guided tissue regeneration and/or Emdogain. A histologic study. J Clin Periodontol. 2003;30:1061-1068   DOI   ScienceOn