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Effect of Treponema lecithinolyticum lipopolysaccharide on matrix metalloproteinase-9 expression  

Nam, Jeong-Ah (Dept of Oral Biology, College of Dentistry, Yonsei University)
Moon, Sun-Young (Dept of Oral Biology, College of Dentistry, Yonsei University)
Lee, Jin-Wook (Dept of Oral Biology, College of Dentistry, Yonsei University)
Cha, Jeong-Heon (Dept of Oral Biology, College of Dentistry, Yonsei University)
Choi, Bong-Kyu (Dept of Oromaxillofacial Infection and Immunity, and Dental Research Institute, College of Dentistry, Seoul National University)
Yoo, Yun-Jung (Dept of Oral Biology, College of Dentistry, Yonsei University)
Publication Information
Journal of Periodontal and Implant Science / v.35, no.3, 2005 , pp. 675-685 More about this Journal
Abstract
Bone resorption involves sequential stages of osteoclast precursor migration and differentiation of osteoclast precursors into multinucleated osteoclasts. Stromal cell derived factor (SDF)-1 is a chemotactic factor for osteoclast precursor migration. Matrix metalloproteinase (MMP)-9 is involved in migration of osteoclast precursors and activation of $interleukin(IL)-1{\beta}$. Alveolar bone destruction is a characteristic feature of periodontal disease. Treponema lecithinolyticum is a oral spirochete isolated from the periodontal lesions. The effect of lipopolysaccharide(LPS) from T. lecithinolyticum on expression of SDF-1 and MMP-9 was examined in cocultures of bone marrow cells and osteblasts derived from mouse calvariae. T. lecithinolyticum LPS increased expression of MMP-9 in the coculture. Polymyxin B, an inhibitor of LPS, abolished the increase of MMP-9 mRNA expression by LPS. LPS did not increase the expression of SDF-1, $IL-1{\beta}$ and tumor necrosis $factor(TNF)-{\alpha}$ mRNA in cocultures. Prostaglandin $E_2(PGE_2)$ up-regulated the expression of MMP-9 and NS398, an inhibitor of $PGE_2$ synthesis, down-regulated the induction of MMP-9 expression by T. lecitbinolyticm LPS. These results suggest that T. lecitbinolyticm LPS increases MMP-9 expression in bone cells via $PGE_2$ and that the induction of MMP-9 expression by T. lecitbinolyticm LPS is involved in alveolar bone destruction of periodontitis patients by the increase of osteoclast precursor migration and the activation of bone resorption-inducing cytokine.
Keywords
Treponema lecithinolyticum; lipopolysaccharide; MMP-9;
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