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MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus  

Kang, Min-Gu (Department of Periodontology, College of Dentistry, Kyungpook National University)
Cha, Hyun-Jeong (Department of Periodontology, College of Dentistry, Kyungpook National University)
Song, Sun-Hee (Department of Periodontology, College of Dentistry, Kyungpook National University)
Park, Jin-Woo (Department of Periodontology, College of Dentistry, Kyungpook National University)
Suh, Jo-Young (Department of Periodontology, College of Dentistry, Kyungpook National University)
Lee, Jae-Mok (Department of Periodontology, College of Dentistry, Kyungpook National University)
Publication Information
Journal of Periodontal and Implant Science / v.35, no.3, 2005 , pp. 661-674 More about this Journal
Abstract
The purpose of this study was to quantify and compare the level of MMP-2, MMP-8 in the healthy, inflammed gingival tissue and inflammed gingival tissue associated with type 2 DM. We investigate whether expression of MMP-2, MMP-8 is increased by chronic periodontitis associated with type 2 DM. Gingival tissue samples were obtained during periodontal surgery or tooth extraction. Based on patient's systemic condition & clinical criteria of gingiva, each gingival samples were divided into three groups. Group l(n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from 8 systemically healthy patients. Group 2(n=8) is inflammed gingiva from patients with chronic periodontitis. Group 3(n=8) is inflammed gingiva from type 2 diabetic patients with chronic periodontitis. Tissue samples were prepared and analyzed by Western blotting. The quantification of MMP-2, MMP-8 was performed using a densitometer and statistically analyzed by ANOVA. MMP-2, MMP-8 was expressed in all samples including healthy gingiva and increased in group 3 compared to group 1 and 2, and showed that significant variation was observed between group 1 & 3 in MMP-8 results. In conclusion, this study demonstrated that human gingival tissue with chronic periodontitis associated to type 2 diabetes showed slightly elevated MMP-2, MMP-8 levels compared to healthy gingiva and non-diabetic inflamed gingiva.
Keywords
Matrix metalloproteinase; MMP-2; MMP-8;
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