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Effect of the combined use of bone morphogenetic protein and platelet-derived growth factor on bone formation in nude mouse  

Lee, Seoung-Ho (Department of Periodontology, Graduate School of Clinical Dentistry, Ewha Woman's University)
Choi, Byung-Ho (Department of Dentistry, Wonju Christian Hospital, Yonsei University)
Zhu, Shi-Jiang (Department of Dentistry, Wonju Christian Hospital, Yonsei University)
Huh, Jin-Young (Department of Dentistry, Gangneung Asan Hospital, University of Ulsan)
Jung, Jae-Hyung (Department of Dentistry, Ilsan Hospital)
Kim, Byung-Yong (Mi plus dental clinic, Seoul)
Publication Information
Journal of Periodontal and Implant Science / v.35, no.2, 2005 , pp. 263-269 More about this Journal
Abstract
Bone morphogenetic protein(BMP) and platelet-derived growth factor(PDGF) have been demonstrated tostimulate bone formation when applied locally in vivo. To explore whether or not the combined use of BMP and PDGF could have promotive effect and synergic interaction on bone formation in vivo, bone marrow mesenchymal stem cells were treated with BMP-2, PDGF-BB, or BMP-2 plus PDGF-BB, and then these cells were injected into the subcutaneous space on the dorsum of nude mice. The bone formation was evaluated after 12 weeks. Histomorphometric analysis demonstrated that the subcutaneous nodules formed in nude mice contained 25.3% newly formed bone in the BMP-2 treated cells, 14.4% newly formed bone in the PDGF-BB treated cells, and 8.9% newly formed bone in the RMP-2 plus PDGF-BB treated cells. The results showed that the combination of BMP-2 and PDGF-BB had neither a promotive effect nor synergic interact on bone formation in vivo.
Keywords
BMP; PDGF; growth factor; bone formation;
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